_|Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria|_

[Giuseppe]

(1.5): Epidemiol Infect. 2008 Jul 17:1-8. [Epub ahead of print]

Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria.

Fasina FO, Bisschop SP, Joannis TM, Lombin LH, Abolnik C. - National Veterinary Research Institute, Vom, Nigeria.

SUMMARY
Avian influenza caused infection and spread throughout Nigeria in 2006.
Carcass samples (lung, liver, spleen, heart, trachea and intestine) from the different regions of Nigeria were processed for virus isolation.
Infective allantoic fluids were tested for avian influenza viruses (AIV) and Newcastle disease virus using monospecific antisera.
Thirty-five isolates were generated and characterized molecularly using the haemagglutinin gene.
The molecular analysis indicated that different sublineages of the highly pathogenic avian influenza (HPAI) H5N1 viruses spread throughout Nigeria.
We compared the Nigerian isolates with others from Africa and results indicated close similarities between isolates from West Africa and Sudan.
Some of the analysed viruses showed genetic drift, and the implications of these for future epidemiology and ecology of avian influenza in Africa require further evaluation.
The spread of primary outbreaks was strongly linked to trade (legal and illegal), live bird markets, inappropriate disposal, and poorly implemented control measures.
No strong correlation existed between wild birds and HPAI H5N1 in Nigeria.
PMID: 18631423 [PubMed - as supplied by publisher]
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http://www.flutrackers.com/forum/showpost.php?p=169825&postcount=1
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[HenryN]

Re: _|Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria|_

(1.5): Epidemiol Infect. 2008 Jul 17:1-8. [Epub ahead of print]

Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria.

Fasina FO, Bisschop SP, Joannis TM, Lombin LH, Abolnik C. - National Veterinary Research Institute, Vom, Nigeria.

SUMMARY
Avian influenza caused infection and spread throughout Nigeria in 2006.
Carcass samples (lung, liver, spleen, heart, trachea and intestine) from the different regions of Nigeria were processed for virus isolation.
Infective allantoic fluids were tested for avian influenza viruses (AIV) and Newcastle disease virus using monospecific antisera.
Thirty-five isolates were generated and characterized molecularly using the haemagglutinin gene.
The molecular analysis indicated that different sublineages of the highly pathogenic avian influenza (HPAI) H5N1 viruses spread throughout Nigeria.
We compared the Nigerian isolates with others from Africa and results indicated close similarities between isolates from West Africa and Sudan.
Some of the analysed viruses showed genetic drift, and the implications of these for future epidemiology and ecology of avian influenza in Africa require further evaluation.
The spread of primary outbreaks was strongly linked to trade (legal and illegal), live bird markets, inappropriate disposal, and poorly implemented control measures.
No strong correlation existed between wild birds and HPAI H5N1 in Nigeria.
PMID: 18631423 [PubMed - as supplied by publisher]
-
http://www.flutrackers.com/forum/showpost.php?p=169825&postcount=1
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All H5N1 in Nigeria is clade 2.2 (which is transported and transmitted by wild birds). Different subclades were are farms 50 miles apart indicating independent introductions (wild birds).

 

[HenryN]

Re: _|Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria|_

Table 1.​

​

List of Nigerian HPAI H5N1 virus isolates included in the study​

Name Species affected Location, state
Date of report/
collection
1 A/chicken/Nigeria/VRD35/2006 Commercial layer chicken ? 2 Feb. 2006
2 A/chicken/Nigeria/VRD42/2006 Commercial layer chicken Katako Area, Plateau 9 Feb. 2006
3 A/chicken/Nigeria/VRD44/2006 Commercial layer chicken Rikkos, Plateau 9 Feb. 2006
4 A/chicken/Nigeria/VRD49/2006 Commercial layer chicken Dahol Giring (Forest),
Plateau
14 Feb. 2006
5 A/chicken/Nigeria/VRD83/2006 Chicken Naraguta, Plateau 17 Feb. 2006
6 A/chicken/Nigeria/VRD91/2006 Commercial layer chicken University Quarters 18 Feb. 2006
7 A/chicken/Nigeria/VRD130/2006 Commercial layer chicken Apollo Cresent, Plateau 24 Feb. 2006
8 A/chicken/Nigeria/VRD130b/2006 Commercial layer chicken Apollo Cresent, Plateau 24 Feb. 2006
9 A/chicken/Nigeria/VRD145/2006 Pullet chicken Sabon Barki, Plateau 26 Feb. 2006
10 A/chicken/Nigeria/VRD146/2006 Commercial layer chicken Katako Market, Plateau 26 Feb. 2006
11 A/chicken/Nigeria/VRD111/2006 Commercial layer chicken Katako Market, Plateau 27 Feb. 2006
12 A/chicken/Nigeria/VRD157/2006 Commercial layer chicken Zaria Road, Plateau 27 Feb. 2006
13 A/chicken/Nigeria/VRD165/2006 Commercial layer chicken Chwelnyap (Congo), Plateau 28 Feb. 2006
14 A/chicken/Nigeria/VRD184/2006 Vulture Vom, Plateau 4 Mar. 2006
15 A/chicken/Nigeria/VRD193/2006 Commercial layer chicken Mando, Kaduna 8 Mar. 2006
16 A/chicken/Nigeria/VRD200/2006 Pullet chicken Jos, Plateau 7 Mar. 2006
17 A/chicken/Nigeria/VRD203/2006 Commercial layer chicken Kaduna, Kaduna 8 Mar. 2006
18 A/chicken/Nigeria/VRD216a/2006 Commercial layer chicken Cooperative Farm,
Agege, Lagos
10 Mar. 2006
19 A/chicken/Nigeria/VRD218/2006 Commercial layer chicken Rikkos, Plateau 11 Mar. 2006
20 A/chicken/Nigeria/VRD219/2006 Commercial layer chicken Old Airport Junction,
Plateau
13 Mar. 2006
21 A/chicken/Nigeria/VRD244/2006 Commercial layer chicken Bukuru, Plateau 30 Mar. 2006
22 A/JWP/Nigeria/VRD252/2006 Wild species Jos Wildlife Park 3 Apr. 2006
23 A/turkey/Nigeria/VRD262/2006 Turkey Ungwan Dosa, Kaduna 6 Apr. 2006
24 A/chicken/Nigeria/VRD284/2006 Commercial layer chicken Apata, Plateau 13 Apr. 2006
25 A/chicken/Nigeria/VRD286/2006 Commercial layer chicken Jos, Plateau 16 Apr. 2006
26 A/chicken/Nigeria/VRD311/2006 Commercial layer chicken Bukuru, Plateau 26 Apr. 2006
27 A/chicken/Nigeria/VRD340/2006 Commercial layer chicken Zaria Road, Plateau 5 May 2006
28 A/turkey/Nigeria/VRD345/2006 Turkey Rukuba, Plateau 8 May 2006
29 A/chicken/Nigeria/VRD368/2006 Commercial layer chicken Dogon Dutse, Plateau 20 May 2006
30 A/pigeon/Nigeria/VRD370/2006 Pigeon Vom, Plateau 20 May 2006
31 A/chicken/Nigeria/VRD403/2006 Commercial layer chicken Molete Market, Oyo 6 June 2006
32 A/duck/Nigeria/VRD418/2006 Duck Dogon Karfe, Plateau 8 June 2006
33 A/chicken/Nigeria/VRD419/2006 Local and commercial
layer chicken
Dogon Karfe, Plateau 8 June 2006
34 A/chicken/Nigeria/VRD457/2006 Commercial layer chicken Ijegun, Lagos 28 June 2006​

35 A/guinea fowl/Nigeria/VRD005/2007 Guinea fowl Kebbe, Sokoto 5 Jan. 2007

 

[HenryN]

Re: _|Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria|_

Gene sequences of selected viruses were deposited
in the Genbank under the accession numbers​

EF631164?EF631187.