Giuseppe
Emeritus
(1.3): J Virol Methods. 2008 Jul 21. [Epub ahead of print]
An improved embryonated chicken egg model for the evaluation of antiviral drugs against influenza A virus.
Wang JX, Zhou JY, Yang QW, Chen Y, Li X, Piao YA, Li HY. - Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, China.
Influenza is a serious global public health problem and an economic burden.
With the continual emergence of new influenza A virus strains, new antiviral drugs are needed urgently.
In this study, an improved embryonated chicken egg model for evaluating antiviral activity against Influenza A virus was developed.
In the model, the influenza A virus was injected into the allantoic cavity and ribavirin was injected into the albumen of the egg.
The levels of influenza A virus in the allantoic fluid was titrated by the hemagglutination test after incubation for 72h at 35.5 degrees C and 12h at 4 degrees C.
Ribavirin treatment at a dose of 25mg/kg to 100mg/kg decreased significantly the hemagglutination titers both of Influenza virus A/FM1, H1N1 (IVA1) (p<0.01) and influenza virus A/Wuhan/359/95, H3N2 (IVA3) (p<0.01).
In a time-dependent drug addition assay, significant efficacy of ribavirin against both IVA1 and IVA3 was observed when the drug was administered before and shortly after viral inoculation (p<0.01 or p<0.05).
In conclusion, ribavirin treatment showed significant antiviral activities against IVA1 and IVA3 in this model, suggesting that the improved model would be useful for evaluating the anti-influenza virus activity of potential inhibitors.
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PMID: 18652846 [PubMed - as supplied by publisher
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An improved embryonated chicken egg model for the evaluation of antiviral drugs against influenza A virus.
Wang JX, Zhou JY, Yang QW, Chen Y, Li X, Piao YA, Li HY. - Department of Epidemiology, Public Health College, Harbin Medical University, Harbin, China.
Influenza is a serious global public health problem and an economic burden.
With the continual emergence of new influenza A virus strains, new antiviral drugs are needed urgently.
In this study, an improved embryonated chicken egg model for evaluating antiviral activity against Influenza A virus was developed.
In the model, the influenza A virus was injected into the allantoic cavity and ribavirin was injected into the albumen of the egg.
The levels of influenza A virus in the allantoic fluid was titrated by the hemagglutination test after incubation for 72h at 35.5 degrees C and 12h at 4 degrees C.
Ribavirin treatment at a dose of 25mg/kg to 100mg/kg decreased significantly the hemagglutination titers both of Influenza virus A/FM1, H1N1 (IVA1) (p<0.01) and influenza virus A/Wuhan/359/95, H3N2 (IVA3) (p<0.01).
In a time-dependent drug addition assay, significant efficacy of ribavirin against both IVA1 and IVA3 was observed when the drug was administered before and shortly after viral inoculation (p<0.01 or p<0.05).
In conclusion, ribavirin treatment showed significant antiviral activities against IVA1 and IVA3 in this model, suggesting that the improved model would be useful for evaluating the anti-influenza virus activity of potential inhibitors.
-
PMID: 18652846 [PubMed - as supplied by publisher
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