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J Infect Dis
. 2024 Jan 23:jiae018.
doi: 10.1093/infdis/jiae018. Online ahead of print. Compartmentalized SARS-CoV-2 replication in upper versus lower respiratory tract after intranasal inoculation or aerosol exposure
Robert J Fischer 1 , Trenton Bushmaker 1 , Brandi N Williamson 1 , Lizzette Pérez-Pérez 1 , Friederike Feldmann 2 , Jamie Lovaglio 2 , Dana Scott 2 , Greg Saturday 2 , Heinz Feldmann 1 , Vincent J Munster 1 , Emmie de Wit 1 , Neeltje van Doremalen 1
Affiliations
Non-human primate models are essential for the development of vaccines and antivirals against infectious diseases. Rhesus macaques are a widely utilized infection model for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We compared cellular tropism and virus replication in rhesus macaques inoculated with SARS-CoV-2 via the intranasal route, or via exposure to aerosols. Intranasal inoculation results in replication in the upper respiratory tract and limited lower respiratory tract involvement, whereas exposure to aerosols results in infection throughout the respiratory tract. In comparison to multi-route inoculation, the intranasal and aerosol inoculation routes result in reduced SARS-CoV-2 replication in the respiratory tract.
Keywords: NHPs; SARS-CoV-2; aerosols; inoculation route; non-human primate; rhesus macaques.
. 2024 Jan 23:jiae018.
doi: 10.1093/infdis/jiae018. Online ahead of print. Compartmentalized SARS-CoV-2 replication in upper versus lower respiratory tract after intranasal inoculation or aerosol exposure
Robert J Fischer 1 , Trenton Bushmaker 1 , Brandi N Williamson 1 , Lizzette Pérez-Pérez 1 , Friederike Feldmann 2 , Jamie Lovaglio 2 , Dana Scott 2 , Greg Saturday 2 , Heinz Feldmann 1 , Vincent J Munster 1 , Emmie de Wit 1 , Neeltje van Doremalen 1
Affiliations
- PMID: 38261786
- DOI: 10.1093/infdis/jiae018
Non-human primate models are essential for the development of vaccines and antivirals against infectious diseases. Rhesus macaques are a widely utilized infection model for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We compared cellular tropism and virus replication in rhesus macaques inoculated with SARS-CoV-2 via the intranasal route, or via exposure to aerosols. Intranasal inoculation results in replication in the upper respiratory tract and limited lower respiratory tract involvement, whereas exposure to aerosols results in infection throughout the respiratory tract. In comparison to multi-route inoculation, the intranasal and aerosol inoculation routes result in reduced SARS-CoV-2 replication in the respiratory tract.
Keywords: NHPs; SARS-CoV-2; aerosols; inoculation route; non-human primate; rhesus macaques.