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Bull Exp Biol Med. 2017 Nov 25. doi: 10.1007/s10517-017-3964-y. [Epub ahead of print]
Comparison of Different Methods of Purification and Concentration in Production of Influenza Vaccine.
Asanzhanova NN1, Ryskeldinova SZ2, Chervyakova OV2, Khairullin BM2, Kasenov MM2, Tabynov KK2.
Author information
Abstract
The overwhelming majority of influenza vaccines are prepared with the use of chicken embryo allantoic fluid. The presence of ovalbumin (this protein constitutes >60% total protein in the allantoic fluid) in the vaccine can lead to severe allergy. Hence, effective reduction of ovalbumin content is of crucial importance for vaccine production. We compared two methods of purification and concentration of influenza virus: zonal gradient ultracentrifugation and combined ultrafiltration/diafiltration and exclusion chromatography protocol, used for fabrication of seasonal vaccines. Combined chromatography is comparable with zonal centrifugation protocol by the results of ovalbumin removal (to meet standard requirements).
KEYWORDS:
chromatography; influenza; purification and concentration; ultracentrifugation; vaccine
PMID: 29177903 DOI: 10.1007/s10517-017-3964-y
Comparison of Different Methods of Purification and Concentration in Production of Influenza Vaccine.
Asanzhanova NN1, Ryskeldinova SZ2, Chervyakova OV2, Khairullin BM2, Kasenov MM2, Tabynov KK2.
Author information
Abstract
The overwhelming majority of influenza vaccines are prepared with the use of chicken embryo allantoic fluid. The presence of ovalbumin (this protein constitutes >60% total protein in the allantoic fluid) in the vaccine can lead to severe allergy. Hence, effective reduction of ovalbumin content is of crucial importance for vaccine production. We compared two methods of purification and concentration of influenza virus: zonal gradient ultracentrifugation and combined ultrafiltration/diafiltration and exclusion chromatography protocol, used for fabrication of seasonal vaccines. Combined chromatography is comparable with zonal centrifugation protocol by the results of ovalbumin removal (to meet standard requirements).
KEYWORDS:
chromatography; influenza; purification and concentration; ultracentrifugation; vaccine
PMID: 29177903 DOI: 10.1007/s10517-017-3964-y