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Published: 02 January 2023
DOI https://doi.org/10.1038/s41587-022-01539-0
Dylan C. Mitchell, Miljan Kuljanin, Jiaming Li, Jonathan G. Van Vranken, Nathan Bulloch, Devin K. Schweppe, Edward L. Huttlin & Steven P. Gygi
Abstract
Defining the cellular response to pharmacological agents is critical for understanding the mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate-based high-throughput screening infrastructure for quantitative proteomics and profiled 875 compounds in a human cancer cell line with near-comprehensive proteome coverage. Examining the 24-h proteome changes revealed ligand-induced changes in protein expression and uncovered rules by which compounds regulate their protein targets while identifying putative dihydrofolate reductase and tankyrase inhibitors. We used protein–protein and compound–compound correlation networks to uncover mechanisms of action for several compounds, including the adrenergic receptor antagonist JP1302, which we show disrupts the FACT complex and degrades histone H1. By profiling many compounds with overlapping targets covering a broad chemical space, we linked compound structure to mechanisms of action and highlighted off-target polypharmacology for molecules within the library.
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https://www.nature.com/articles/s41587-022-01539-0#citeas
DOI https://doi.org/10.1038/s41587-022-01539-0
Dylan C. Mitchell, Miljan Kuljanin, Jiaming Li, Jonathan G. Van Vranken, Nathan Bulloch, Devin K. Schweppe, Edward L. Huttlin & Steven P. Gygi
Abstract
Defining the cellular response to pharmacological agents is critical for understanding the mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate-based high-throughput screening infrastructure for quantitative proteomics and profiled 875 compounds in a human cancer cell line with near-comprehensive proteome coverage. Examining the 24-h proteome changes revealed ligand-induced changes in protein expression and uncovered rules by which compounds regulate their protein targets while identifying putative dihydrofolate reductase and tankyrase inhibitors. We used protein–protein and compound–compound correlation networks to uncover mechanisms of action for several compounds, including the adrenergic receptor antagonist JP1302, which we show disrupts the FACT complex and degrades histone H1. By profiling many compounds with overlapping targets covering a broad chemical space, we linked compound structure to mechanisms of action and highlighted off-target polypharmacology for molecules within the library.
This is a preview of subscription content, access via your institution
https://www.nature.com/articles/s41587-022-01539-0#citeas