Biomol NMR Assign . The 1 H, 15 N, and 13 C resonance assignments of the N-terminal domain of the nucleocapsid protein from the Middle East respiratory syndrome coronavirus

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Biomol NMR Assign


. 2021 Apr 29.
doi: 10.1007/s12104-021-10027-6. Online ahead of print.
The ¹ H, ¹⁵ N, and ¹³ C resonance assignments of the N-terminal domain of the nucleocapsid protein from the Middle East respiratory syndrome coronavirus


Talita Stelling de Araujo ^{1 2} , Glauce Moreno Barbosa ^{3 2} , Karoline Sanches ^{3 2} , J?ssica M Azevedo ^{1 2} , Katia Maria Dos Santos Cabral ^{1 2} , Marcius S Almeida ^{1 2} , Fabio C L Almeida ^{4 5}



Affiliations

• PMID: 33914244
• DOI: 10.1007/s12104-021-10027-6

Abstract

During the past 17 years, the coronaviruses have become a global public emergency, with the first appearance in 2012 in Saudi Arabia of the Middle East respiratory syndrome. Among the structural proteins encoded in the viral genome, the nucleocapsid protein is the most abundant in infected cells. It is a multifunctional phosphoprotein involved in the capsid formation, in the modulation and regulation of the viral life cycle. The N-terminal domain of N protein specifically interacts with transcriptional regulatory sequence (TRS) and is involved in the discontinuous transcription through the melting activity of double-stranded TRS (dsTRS).

Keywords: Coronavirus; MERS-CoV; N protein; NMR; Nucleocapsid; Nucleoprotein.