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Mem Inst Oswaldo Cruz
. 2020 Sep 25;115:e200310.
doi: 10.1590/0074-02760200310. eCollection 2020.
Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil
Valdinete Alves do Nascimento 1 2 , Andr? de Lima Guerra Corado 1 2 , Fernanda Oliveira do Nascimento 1 3 , ?gatha Kelly Ara?jo da Costa 1 3 , Debora Camila Gomes Duarte 1 , S?rgio Luiz Bessa Luz 1 3 , Luciana Mara F? Gon?alves 4 , Michele Silva de Jesus 1 , Cristiano Fernandes da Costa 4 , Edson Delatorre 5 , Felipe Gomes Naveca 1 2 3 6
Affiliations
- PMID: 32997001
- DOI: 10.1590/0074-02760200310
Abstract
A new coronavirus [severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)] is currently causing a life-threatening pandemic. In this study, we report the complete genome sequencing and genetic characterisation of a SARS-CoV-2 detected in Manaus, Amazonas, Brazil, and the protocol we designed to generate high-quality SARS-CoV-2 full genome data. The isolate was obtained from an asymptomatic carrier returning from Madrid, Spain. Nucleotide sequence analysis showed a total of nine mutations in comparison with the original human case in Wuhan, China, and support this case as belonging to the recently proposed lineage A.2. Phylogeographic analysis further confirmed the likely European origin of this case. To our knowledge, this is the first SARS-CoV-2 genome obtained from the North Brazilian Region. We believe that the information generated in this study may contribute to the ongoing efforts toward the SARS-CoV-2 emergence.