H7n9

[gsgs]

H7-cleavage sites
------------------------

first I found that there is a 8aa deletion in H7 at 230-237.
I didn't know that. 388 H7s at genbank had the deletion and 884 had not
The deletion is only in American H7N2.
So I excluded these.

    331,KG
    302,KT
     22,KP
      7,KR
      6,KA
      5,RG
      4,RK
      3,KE
      4,KK
      1,XG
      1,KRR
     30,KRRK
     16,KRRR
      6,RKRK
      4,KKKK
      2,RRRK
      2,KRKK
      1,KRRN
      9,KKREK
      7,KKRRK
      5,KKRKK
      2,KRKRK
     33,KGSRVR
      2,KKKKKK
      1,KGTKPRPR
      1,KTTKPRPR
      4,KQAYRKRMT
      2,KQAYQKRMT
      2,KQAYQKQMT
      2,KQAHQKRMT
      1,KQAYKKRMT
      1,KQAYHKRMT
      1,KQACQKRMT
      1,RQAYRKRMT
      5,KDRKSRHRRT
     16,THKQLTHHMRKK
      5,AHKQLTHHMRKK
      5,KTCSPLSRCRET
      3,KTCSPLSRCRKT
      1,IHKQLTHHMRKK

[gsgs]

Re: H7n9

5aa deletion in N9 at (nucleotide-?) position 205
not seen before

maybe we can compare it with deletions in other NAs

[gsgs]

Re: H7n9

H7-receptor binding domains (rbd)

aa-positions 98,134-138,153-155,184-196,216-230 in H3-numbering
(as in H1 or H3, it could be different in H7)

Y GVTAA WIT HPPTTSEQQSLYQ EIAARPKVRGQAGRM

A/turkey/MN/1661/1981(H1N1)

aligning H1 to H7 ...


-------------------------------------------------------------------------------------------

receptor binding domain of influenza-A viruses

positions 98,134-138,153-155,184-196,216-230 in hemagglutinin
(H3-numbering)


count,species-serotype,amino-acids at these positions,representative sequence
-----------------------------------------------------------------------------------------------------
                 Y GVTAA WIT HPPTTSEQQSLYQ EIAARPKVRGQAGRM
   9 A-H1N1:     Y GVTAA WIT HPPTTSEQQSLYQ EIAARPKVRGQAGRM     A/turkey/Minnesota/1661/81(H1N1)
   4 A-H1N1:     . ..... ... .....N....... ...............    A/pintail duck/Alberta/210/2002(H1N1)

                .....S.S..L.....NIGD.RA..H....K.....D.E..I
 325 H-H1N1:     Y GVSAS WLT HPPNIGDQRALYH EIAKRPKVRDQEGRI     A/Wyoming/4/2003(H1N1)
  64 H-H1N1:     . ..T.. ... ..S.......I.. ...............    A/Switzerland/5389/95 (H1N1)
  44 H-H1N1:     . ..... ... ......N...... ...............    A/Yokohama/62/200

                ....A.....LN..SESVS..TK....SPGA.--------..
  39 A-H7N2:     Y GATSA WLN HSESVSEQTKLYG SPGAR--------RI     A/turkey/VA/67/02(H7N2)
  22 A-H7N1:     . ..... ... ..G.TT....... .....PQVNGQSG..    A/turkey/Italy/977/99 (H7N1)
  22 A-H7N1:     . .T..T ... ..G.TT....... ...E.PQVNGQSG..    A/turkey/Italy/5074/99 (H7N1)
  20 A-H7N7:     . ..... ... ..G.TT....... .....PQVNGQSG..    A/turkey/Germany/R11/01(H7N7)
  15 A-H7N3:     . ..... ... ..G.TT....... .....PQVNGQSG..    A/turkey/Oregon/1971(H7N3)
  14 A-H7N3:     . ..... ... ..G.AT....... .....PQVNGQSG..    A/Turkey/Colorado/13356/91 (H7N3)
   8 A-H7N2:     . .T... ... ............. .T.............    A/Chicken/Pennsylvania/13552-1/98 (H7N2)
   8 A-H7N7:     . ...N. ... ..G.TT....... .....PQVNGQSG..    A/Mallard/Sweden/93/02(H7N7)
   7 A-H7N1:     . ..... ... ..G.TA....... .....PQVNGQSG..    A/parrot/England/1174/94(H7N1)
   7 A-H7N2:     . ..... ... ..G.......... .....PQVNGQSG..    A/Chicken/Rhode Island/4328/95 (H7N2)
   6 A-H7N3:     . .V... ... ..G.AN....... .....PQVNGQSG..    A/turkey/Chile/4418/02(H7N3)
   5 A-H7N1:     . .T... ... ..G.TT....... .....PQVNGQSG..    A/turkey/Italy/4857/99 (H7N1)
   4 A-H7N1:     . ..... ... ..V.TT....... .L...PQVNGQSG..    A/turkey/Italy/4042/99 (H7N1)
   4 A-H7N1:     . ..... ... ..V.TT....... .....PQVNGQSG..    A/turkey/Italy/2715/99 (H7N1)
   4 A-H7N2:     . ..... ... ............. N..............    A/guineafowl/NY/4649-18/2006(H7N2)
   4 A-H7N3:     . ..... ... ..G.AN....... .....PQVNGQSG..    A/Quail/Arkansas/16309-7/94 (H7N3)
   4 A-H7N3:     . ....S ... ..G.TT...R... .....PQVNGQSG..    A/peregrine falcon/U.A.E./188/2384/98(H7N3)
   4 A-H7N7:     - ----- --- ..G.TT....... .....PQVNGQSG..    A/Mongolia/736/02(H7N7)

                ....E..........G.TA...R....N..P.PQINGQSG..
   6 E-H7N7:     Y GETSA WLN HSGSTAEQTRLYG NPGPRPQINGQSGRI     A/equine/Sao Paulo/1/76(H7N7)

                ....A......S......T...K....S..A...V.......
   1 H-H7N1:     Y GATSA WLS HSGSTTEQTKLYG SPGARPQVNGQSGRI     A/Hong Kong/301/78(H7N1)

                ....T......N..........R...................
   1 S-H7N7:     Y GTTSA WLN HSGSTTEQTRLYG SPGARPQVNGQSGRI     A/seal/Mass/1/80(H7N7)

                .....SK....K..PPD.K...T..K.NI.P..L.R..Q..M
   7 A-H8N4:     Y GTSKA WLK HPPDTKEQTTLYK NIGPRPLVRGQQGRM     A/Turkey/Ontario/6118/68(H8N4)

                ...........T....TDTA..N..T.L.......N.L...I
  39 A-H9N2:     Y GTSKA WLT HPPTDTAQTNLYT LIGPRPLVNGLQGRI     A/Silky Chicken/Hong Kong/SF44/99(H9N2)
  17 A-H9N2:     . ..... ... ............I V......A.......    A/turkey/Tzur Moshe/1565/04(H9N2)

    187,GATSA WLL HSGSTTEQTKLYGSG SPGARPQVNGQSGRI 
    135,----- --- ----A---------- --------------- 
     68,----- --- ----AD--------- --------------- 
     44,----- --- -----A--------- --------------- 
     37,-T--T --- --------------- ---E----------- 
     34,----- --- ----VS--------- --------------- 
     22,----- --- --V------------ --------------- 
     21,-T--- --- --------------- --------------- 
     16,-V--- --- ----A---------- --------------- 
     16,----- --- ----AN--------- --------------- 
     12,-T--S --- ---------R----- --------------- 
     12,----S --- ---------R----- --------------- 
     11,----- --- --V----------G- -L------------- 
     10,-V--- --- --------------- --------------- 
     10,----- --- --------A------ --------------- 
      9,----- --- -------------G- --------------- 
      8,-E--- --- -----A---R----- N--P---I------- 
      8,---N- --- --------------- --------------- 
      7,-V--- --- ----AN--------- --------------- 
      7,----- --- --------------- --E------------ 
      6,-T--S --- ---------R----- ---V----------- 
      6,-E--- --- -----A---R----- ---P---I------- 
      6,----- --- --V-A---------- --------------- 
      6,----- --- ----A---------- ------P--------

RBD in flugenome.org-types
---------------------------
41A,EIAARPKVRGQAGRM, 235  8 
41B,EIAKRPKVRDQEGRI, 235  7 
41C,EIAARPKVRGQAGRM, 235  8 
41D,EIAARPKVRGQAGRM, 235  8 
41E,ELAARPKVRGQAGRI, 235  9 
42A,EIATRPKVNGLGSRM, 231  6 
42B,EIATRPNVNGQGGRM, 231  8 
42C,EIATRPKVNGQRGRM, 231  8 
42D,EIATRPKVNGQGGRM, 231  8 
42E,EIATRPKVNGQGGRM, 231  8 
42F,EIATRPKVNGQGGRM, 231  8 
42G,EIATRPKVNGQGGRM, 231  8 
42H,EIATRPKVNGQGGRM, 231  8 
42I,EIATRPAVNGQRGRM, 231  8 
43A,NIGSRPRVRDVSSRI, 237  7 
43B,NIGSRPWVRGQSGRI, 237  10 
43C,NIGSRPWVRGQSGRI, 237  10 
43D,NIGSRPWVRGQSGRI, 237  10 
43E,NIGSRPWVRGQSGRI, 237  10 
43F,NIGSRPWVRGQSGRI, 237  10 
44A,NIGSRPWVRGQSGRI, 234  10 
44B,NIGSRPWVRGQSGRI, 234  10 
44C,NIGSRPWVRGQSGRI, 234  10 
44D,NIGSRPWVRGQSGRI, 234  10 
45A,KIATRPKVNGQSGRM, 233  9 
45B,EIATRPKVNGQSGRM, 233  9 
45C,EIATRPKVNGQSGRM, 233  9 
45D,EIATRPRVNGQYGRI, 233  9 
45E,EIATRPKVNGQSGRM, 233  9 
45F,EIATRPKVNGQSGRM, 233  9 
45G,EIATRPKVNGQSGRM, 233  9 
45H,EIATRPKVNGQSGRM, 233  9 
45I,EIATRPKVNGQSGRM, 233  9 
45J,KIATRSKVNGQSGRM, 233  8 
45K,EIATRPKVNGQSGRM, 233  9 
46A,EIAARPAVNGQRGRI, 235  10 
46B,EIAARPAVNGQRGRI, 235  10 
46C,EIAARPAVNGQRGRI, 235  10 
46D,EIAARPAVNGQRGRI, 235  10 
46E,EIAARPAVNGQRGRI, 235  10 
46F,EIASRPPVNGQRGRI, 235  9 
46G,EIADRPPANGQRGRI, 235  8 
47A,SPGARPQVNGQSGRI, 230  15 
47B,SPGTRPQVNGQSGRI, 230  14 
47C,SPGARPQVNGQSGRI, 230  15 
47D,NPGPRPQINGQSGRI, 230  12 
47E,SPGARPQVNGQSGRI, 230  15 
47F,SPGAR--------RI, 230  7 
48A,NIGPRPLVRGQQGRM, 234  8 
49A,VIGPRPLVNGHKGRI, 229  9 
49B,VIGPRPLVNGLQGRI, 229  9 
49C,LIGPRPLVNGLQGRI, 229  9 
49D,VIGPRPLVNGQQGRI, 229  10 
49E,VIGPRPLVNGQQGRI, 229  10 
49F,VIGPRPLVNGQQGRI, 229  10 
49G,VIGPRPLVNGQQGRI, 229  10 
49H,MIGPRPLVNGQQGRI, 229  10 
49I,VIGPRPLVNGQQGRI, 229  10 
49J,VIGPRPLVNGQQGRI, 229  10 
4AA,VVGARPQVNGQSGRI, 231  13 
4AC,VVGARPQVNGQSGRI, 231  13 
4AD,VVGARPQVNGQSGRI, 231  13 
4AE,VVGARPQVNGQSGRI, 231  13 
4AF,VVGARPQVNGQSGRI, 231  13 
4BA,EISTRPKVNGQAGRM, 233  8 
4BB,EINTRPRVNGQAGRM, 233  8 
4BC,EISTRPKVNGQAGRM, 233  8 
4CA,NIGPRPLVRGQQGRM, 233  8 
4CB,NIGPRPLVREQQGRM, 233  7 
4DA,ETGVRPGYNGQRSWM, 234  6 
4DB,EIGIRPGYNGQKSWM, 234  6 
4DC,ETGVRPGYNGQRSWM, 234  6 
4EA,NIGSRPRVRNQSGRI, 238  9 
4EB,NIGSRPRVRNQSGRI, 238  9 
4FA,SPGARPKVNGQAGRI, 230  13 



42A,EIATRPKVNGLGSRM, 231  6 
43A,NIGSRPRVRDVSSRI, 237  7 

49A,VIGPRPLVNGHKGRI, 229  9 
49B,VIGPRPLVNGLQGRI, 229  9 
49C,LIGPRPLVNGLQGRI, 229  9 





4AB,IIGAPACDLHLTGRW, 82  4 
4GA,HTGTYCSLNGVSPIH, 61  4 
4GB,RPGERIMFESNGGLI, 256  5 
4GC,YPVVRGDYNNTTGRD, 180  5

[gsgs]

Re: H7n9

snippets:

WHO update: http://www.who.int/csr/don/2013_04_07/en/index.html

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3165745/

A/Quail/Hong Kong/G1/1997 (H9N2) (H9N2/G1) with D253N and Q591K in the PB2

http://www.ncbi.nlm.nih.gov/pubmed/22416344

Q225L




2 other papers about h9n2 evolution

http://www.ncbi.nlm.nih.gov/pubmed/22416344

Samples of chicken, duck, quail, and pigeon were collected from Jiangsu, Anhui,
and Hebei in 2009-2011
amino acid motif of cleavage sites in the HA gene was P-S-R/K-S-S-R
Leucine (L) at the amino acid position 226 in the HA
AIVs originated from F98-like virus as backbone and formed two new genotypes through
reassortment with HA gene of Y280-like virus and PB2 and M genes of G1-like virus

----------------------------------------------------------------------

http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049224

there is variable homology among the various NA subtype sequences, especially
in the N1 and N2 subtypes with the deletion of 4~30 amino acids in the stalk domain [23,1993]
Meanwhile, the factors that influence NA include deletion of the stalk domain,
the mutations drug-resistance, as well as the changing of antigen sites or glycosites
and the variation of the topology of N-glycan structures [28,2011].
The pattern of stalk domain deletion in N2 NA is distinctive in different combinations of IVs.
The representative NA sequences in each available N2 subtypes are aligned in the stalk domain.
As can be observed, the number of deletions varies from 4 to 24 residues,
causing the loss of one or two potential glycosites.
9 NA subtypes are concentrated into two evolutionary groups: one group was represented by
N2 and contains N3, N6, N7, and N9; and another group contains N1, N4, N5, and N8.

deletion of 3 residues and one corresponding glycosite with the pattern “E-R-61N-3-64T-V-H”
(meaning 3 residues missing between 61N and 64T, e.g., A/chicken/Zhejiang/611/2011 (H9N2)),
appeared in most of the NA subtypes of the H9N2 virus.

---------------------------------------------------------------

[gsgs]

Re: H7n9

the Shanghai/Ci {i=1..3} H9N2 chicken sequences from Jan.2012
look boguous.
Lots of clusters of mutations at neighboring nucleotides,
which is unusual
However, the other mutations look reasonable, so maybe
we should just delete all mutation-clusters in them ?!
C1 and C3 have N66S in PB1-F2

>A/chicken/Shanghai/C1/2012,2012/01/,H9N2
>A/chicken/Shanghai/C2/2012,2012/01/,H9N2
>A/chicken/Shanghai/C3/2012,2012/01/,H9N2

also some strange (pseudo)recombinations in the Zhejiang chickens
A/chicken/Zhejiang/329/2011(H9N2)
A/chicken/Zhejiang/607/2011(H9N2)

http://www.ncbi.nlm.nih.gov/nuccore/KC417064

REFERENCE 1 (bases 1 to 2274)
AUTHORS Ge,F.
TITLE Genetic variation of H9N2 subtype avian influenza virus isolates
from diseased chicken flocks of Shanghai in 2012
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2274)
AUTHORS Ge,F.
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2012) Veterinary Disease Diagnostic Center,
Shanghai Animal Disease Control Center, No. 30 Lane 855 Hongjing
Road Changning DC, Shanghai, Shanghai 201103, China
COMMENT GenBank Accession Numbers KC417046, KC417049, KC417052, KC417055,
KC417058, KC417061, KC417064, KC417067 represent sequences from the
8 segments of Influenza A virus (A/chicken/Shanghai/C1/2012(H9N2)).
Sequencing Technology :: Sanger dideoxy sequencing

--------------------------------------------------------------------------------------------------------------------------------

AUTHORS Wu,H. and Wu,N.
TITLE Genetic characterization of subtype H9 avian influenza viruses in
Zhejiang, China
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1497)
AUTHORS Wu,H. and Wu,N.
TITLE Direct Submission
JOURNAL Submitted (05-JAN-2012) State Key Laboratory for Diagnosis and
Treatment of Infectious Diseases First Affiliated Hospital, School
of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou,
Zhejiang 310003, China
COMMENT GenBank Accession Numbers JQ356872, JQ356875, JQ356878, JQ356881,
JQ356884, JQ356887, JQ356890, JQ356893 represent sequences from the
8 segments of Influenza A virus
(A/chicken/Zhejiang/329/2011(H9N2)).

------------------------------------------------------------------------------------------------------------------------------------

AUTHORS Wu,H. and Wu,N.
TITLE Genetic characterization of subtype H9 avian influenza viruses in
Zhejiang, China
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 2151)
AUTHORS Wu,H. and Wu,N.
TITLE Direct Submission
JOURNAL Submitted (05-JAN-2012) State Key Laboratory for Diagnosis and
Treatment of Infectious Diseases First Affiliated Hospital, School
of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou,
Zhejiang 310003, China
COMMENT GenBank Accession Numbers JQ356873, JQ356876, JQ356879, JQ356882,
JQ356885, JQ356888, JQ356891, JQ356894 represent sequences from the
8 segments of Influenza A virus
(A/chicken/Zhejiang/607/2011(H9N2)).

------------------------------------------------------------------

>A/chicken/Shanghai/C1/2012,2012/01/,H9N2,segment 5
>A/chicken/Jiangsu/Q3/2010,2010/02/,China,H9N2,segment 5
.....................................................o...... 1 
........o................................o.................. 2 
............................................................ 0 
............................................................ 0 
.........................................................o.. 1 
............................................................ 0 
.....o.............................o.....o.................. 3 
............................................................ 0 
............................................................ 0 
......o...o...o..o..........ooo......oo..............o...... 10 
.........o...............................o.....o............ 3 
......................................................o..... 1 
................................ooo............o............ 4 
...................................o.o...................... 2 
...........................oo....oo......................... 4 
.......................................oo......ooo......ooo. 8 
...........o.o..........................o.............oo.... 5 
..oo................o......................................o 4 
oo.....................ooo..................o...........ooo. 9 
........ooo................................................. 3 
...............o............................................ 1 
...................................o....o...oo........oo.... 6 
.....ooo.................................................... 3 
............................................................ 0 
.........................................................-- 70 


 70 ( 1497 )+ 70 ( 1497 )

[gsgs]

Re: H7n9

H9N2 East-China Index (preliminary)

>A/poultry/index/East-China/2010,2009//,H9N2
ATGGAAAGAATAAAAGAACTAAGAGATTTGATGTCACAGTCTCGCACTCGCGAGATACTGACAAAAACAACTGTGGACCATATGGCCATAATCAAGAAATATACATCGGGAAGACAGGAGAAGAATCCTGCCCTTAGGATGAAGTGGATGATGGCGATGAAATATCCAATTACAGCAGACAAAAGGATAATGGAGATGATCCCGGAAAGAAATGAGCAAGGTCAGACCCTTTGGAGCAAGACAAATGATGCTGGATCAGACAGAGTGATGGTGTCACCTCTGGCTGTGACGTGGTGGAACAGAAATGGACCAACGACAAGTACAGTCCATTATCCAAAGGTCTATAAAACCTATTTTGAAAAGGTCGAAAGGCTAAAACATGGAACCTTCGGCCCCGTTCACTTCCGAAACCAGGTTAAAATACGCCGCAGGGTCGACATAAACCCAGGCCATGCAGATCTTAGTGCTAAAGAAGCACAAGATGTCATCATGGAGGTCGTATTCCCAAACGAAGTTGGAGCCAGAATATTGACATCAGAGTCACAGTTAACGATAACCAAGGAAAAGAAGGAGGAGCTTCAGGACTGCAAAATTGCCCCTTTAATGGTGGCCTACATGTTGGAGAGAGAACTGGTTCGCAAAACAAGATTTCTACCAGTAGCTGGAGGGACAAGCAGTGTGTATATCGAAGTGTTGCATTTGACCCAAGGGACCTGCTGGGAGCAAATGTACACACCGGGAGGGGAAGTGAGAAATGATGATGTTGATCAGAGTTTAATTATTGCTGCTAGAAATATTGTTAGAAGAGCAACAGTATCAGCAGACCCGTTGGCTTCGCTTTTGGAGATGTGCCATAGTACACAGATTGGCGGGGTTAGGATGGTTGACATCCTTAGACAAAACCCAACAGAAGAACAGGCTGTGGATATATGCAAGGCAGCAATGGGTCTAAGGATCAGTTCATCCTTCAGCTTTGGAGGTTTCACTTTCAAAAGGACAAGTGGGTCATCTGTCAAAAGGGAAGAAGAAGTGCTCACAGGCAACCTCCAAACATTGAAAATAAGAGTACATGAAGGATATGAGGAATTCACAATGGTCGGGCGAAGAGCAACAGCCATTCTAAGGAAAGCAACCAGAAGACTGATCCAACTGATAGTGAGTGGGAAAGACGAGCAATCAATCGCCGAGGCAATCATAGTGGCAATGGTGTTCTCACAAGAGGATTGTATGATAAAGGCAGTGAGAGGTGATTTGAACTTTGTCAACAGAGCGAACCAGCGGCTAAATCCCATGCATCAACTCCTGAGGCATTTCCAAAAGGATGCAAAGGTTCTGTTTCAAAACTGGGGAATTGAACCCATTGACAATGTAATGGGGATGATCGGAATATTGCCTGACATGACCCCCAGCACAGAGATGTCATTGAGAGGAGTGAGAGTTAGTAAAATGGGAGTAGATGAATATTCCAGTACTGAGAGAGTGGTCGTGAGTATTGATCGTTTCTTGAGGGTCCGAGACCAGAGGGGAAACGTACTCCTGTCTCCTGAAGAGGTTAGTGAAACACAGGGAACAGAAAAGCTGACTATAACATATTCATCGTCCATGATGTGGGAGATCAATGGTCCGGAATCAGTGCTAGTTAACACATATCAATGGATCATTAGAAATTGGGAAACTGTAAAGATTCAATGGTCCCAAGACCCTACAATGCTATACAATAAGATGGAGTTTGAACCCTTTCAATCCCTAGTGCCTAAAGCTGCCAGAGGCCAATATAGTGGGTTCGTAAGGGTGCTATTCCAGCAGATGCGTGACGTACTGGGGACATTTGATACTGTCCAAATAATAAAGCTATTACCATTTGCAGCAGCCCCGCCGGAGCAGAGTAGGATGCAGTTCTCTTCTCTAACTGTGAACGTGAGGGGTTCAGGAATGAGAATAGTTGTGAGAGGCAATTCTCCTGTGTTCAACTACAACAAGGCAACCAAGAGGCTTACAGTGCTTGGAAAGGATGCAGGTGCATTGATGGAAGACCCAGATGAGGGAACAGCAGGAGTGGAATCTGCGGTATTGAGAGGATTTCTGATTCTGGGCAAAGAAGACAAAAGATATGGGCCAGCATTGAGCATCAACGAATTGAGCAATCTTGCGAAAGGGGAGAAGGCTAATGTGTTGATAGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACTGACAGTCAGACAGCGACCAAAAGGATTCGGATGGCCATCAATTAA
ATGGATGTCAATCCGACTTTACTTTTCTTGAAAGTGCCAGTGCAAAATGCTATAAGTACCACGTTCCCTTATACTGGAGACCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATAAATACTCAGAAAAAGGAAAGTGGACAACGAACACAGAGACTGGAGCACCCCAACTCAATCCGATTGATGGACCATTGCCTGAGGACAACGAGCCGAGTGGGTATGCACAAACGGATTGTGTATTGGAAGCAATGGCTTTCCTTGAAGAATCTCACCCAGGGATCTTTGAAAACTCGTGTCTCGAAACGATGGAAATTGTTCAGCAAACAAGAGTGGATAAACTGACCCAAGGCCGCCAGACCTATGACTGGACGTTGAATAGAAATCAGCCGGCTGCTACTGCATTGGCCAACACTATAGAGGTATTCAGATCGAATGGCCTGACAGCCAATGAATCAGGAAGGTTGATCGATTTCCTCAAGGACGTGATGGATTCAATGGATAAGGAAGAAATGGAGATTACAACACATTTCCAGAGGAAGAGGAGAGTGAGGGACAACATGACCAAGAAAATGGTCACACAGAGAACAATAGGAAAGAAAAAACAAAGACTGAACAAAAGGAGCTACCTAATAAGAGCACTGACATTGAACACAATGACAAAGGATGCTGAAAGAGGCAAGTTGAAAAGGAGGGCAATCGCAACACCCGGGATGCAAATCAGAGGATTCGTGTATTTTGTAGAAGCACTAGCGAGGAGCATCTGTGAGAAACTTGAGCAATCTGGCCTCCCTGTCGGAGGGAATGAGAAGAAAGCTAAATTGGCAAATGTTGTGAGGAAGATGATGACTAATTCACAAGATACAGAGCTCTCCTTCACAATTACTGGAGACAACACCAAATGGAATGAGAATCAAAACCCCCGGATGTTTCTAGCAATGATAACATACATCACAAGAAACCAGCCAGAATGGTTTAGAAATGTCTTAAGCATTGCTCCTATAATGTTCTCAAACAAGATGGCGAGATTAGGGAAAGGATACATGTTCGAAAGTAAGAGTATGAAGTTACGGACACAAGTACCAGCGGAAATGCTCGCAAATATTGACCTGAAATACTTCAACAAATCAACAAGAGAGAAAATCGAGAAAATAAGACCTCTACTGATAGATGGCACAGCCTCATTGAGTCCTGGAATGATGATGGGCATGTTCAACATGTTGAGTACAGTCTTAGGAGTTTCAATTCTGAATCTCGGGCAGAAGAAGTACACCAAAACCACATATTGGTGGGACGGACTCCAATCCTCAGATGACTTCGCCCTCATAGTGAATGCACCGAATCATGAGGGAATACAGGCAGGAGTGGATAGGTTCTATAGAACCTGCAAATTAGTTGGGATAAACATGAGCAAGAAGAAATCCTACATAAATCGGACAGGAACATTCGAATTCACAAGCTTTTTCTACCGCTATGGATTCGTAGCTAACTTCAGTATGGAGTTGCCCAGTTTTGGAGTGTCTGGGATTAATGAGTCAGCTGACATGAGCATTGGTGTTACAGTAATAAAGAACAATATGATAAACAACGATCTTGGACCAGCAACAGCTCAGATGGCCCTTCAGCTATTTATCAAAGACTACAGATACACATACCGATGTCACAGGGGTGATACGCAAATTCAAACGAGGAGAGCATTCGAGCTGAAGAAGCTGTGGGAGCAGACCCGTTCGAAGGCAGGACTGTTGGTTTCAGATGGAGGGCCAAACCTGTACAATATCCGGAACCTCCACATTCCAGAGGTCTGCTTGAAATGGGAATTGATGGATGAAGACTACCAAGGCAGGCTGTGTAATCCTATGAACCCGTTTGTCAGTCATAAGGAAATTGATTCCGTCAACAATGCTGTGGTGATGCCAGCTCATGGCCCAGCCAAAAGCATGGAGTATGATGCCGTTGCAACTACACATTCATGGATTCCTAAGAGGAATCGCTCCATTCTCAACACCAGCCAAAGGGGGATTCTTGAGGATGAACAGATGTACCAGAAGTGCTGCAACCTATTCGAAAAGTTCTTCCCCAGCAGTTCATACCGGAGGCCAGTTGGAATTTCCAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGAATTGATGCACGAATTGACTTCGAATCTGGAAGGATTAAGAAAGAAGAGTTTGCTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCTCAGACGGCAAAAATAG
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACGAACAAATTCGCATCAATATGCACACACTTAGAAGTCTGCTTCATGTACTCTGATTTCCACTTCATTGACGAACGAGGCGAATCAACTATTATAGAATCTGGCGATCCAAATGCGTTGTTGAAACACCGATTTGAAATAATCGAAGGGAGAGACCGAACAATGGCCTGGACAGTGGTGAATAGTATCTGCAACACCACGGGAGTCGAGAAACCCAAATTTCTTCCGGATCTGTATGACTACAAGGAAAACCGATTCATTGAAATTGGAGTAACGAGGAGGGAAGTCCACATATACTACCTAGAGAAAGCCAATAAAATAAAATCCGAGAAGACACACATCCACATTTTCTCATTCACTGGAGAAGAGATGGCCACCAAAGCAGATTACACTCTTGACGAAGAAAGCAGGGCAAGAATCAAAACCAGGCTGTTCACCATAAGGCAGGAAATGGCCAGCAGGGGTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAAACAATTGAAGAAAGATTTGAAATCACAGGAACCATGCGCAGGCTTGCCGACCAAAGTCTCCCACCGAACTTCTCCAGCCTTGAAAACTTTAGAGCCTATGTGGATGGATTCGAACCGAACGGCTGCATTGAGGGCAAGCTTTCTCAAATGTCAAAAGAAGTGAACGCCAGAATCGAGCCATTTCTAAGGACAACACCACGCCCTCTCAGATTGCCTAATGGGCCTCCCTGCTCTCAGCGGTCGAAATTCTTGCTGATGGATGCTCTGAAATTAAGCATTGAGGACCCGAGCCACGAAGGGGAGGGGATACCGCTATATGATGCGATCAAATGCATGAAAACGTTCTTCGGGTGGAAAGAGCCCAACATTATCAAACCACATGAGAAAGGCATAAACCCCAATTATCTCCTGACTTGGAAGCAGGTGCTAGCAGAACTTCAGGACATTGAAAATGAAGAGAAGATTCCAAAGACAAAGAACATGAAGAAAACAAGCCAATTAAAGTGGGCACTCGGTGAGAACATGGCACCGGAGAAGGTGGACTTTGAAGATTGCAAAGATGTCAACGACCTGAAACAGTACGACAGTGATGAGCCAGAGCCCAGATCACTAGCATGTTGGATCCAGAATGAATTCAACAAGGCATGTGAATTGACTGATTCAAGCTGGGTAGAACTTGATGAAATAGGGGAAGATGTTGCCCCAATCGAACACATTGCAAGCATGAGACGGAACTATTTTACAGCAGAGGTGTCCCACTGCAGGGCTACTGAATACATAATGAAGGGAGTGTACATAAATACAGCTTTGCTCAATGCATCTTGTGCGGCCATGGATGACTTTCAACTGATTCCAATGATAAGCAAATGCAGAACCAAAGAAGGAAGACGGAAAACAAACCTATATGGATTCATTATAAAAGGAAGATCTCATTTGAGGAATGATACCGACGTGGTAAACTTTGTAAGTATGGAATTTTCCCTTACCGACCCAAGGTTGGAACCACATAAATGGGAAAAGTATTGTGTTCTTGAAATAGGGGACATGCTCCTGCGAACTGCAGTAGGCCAAGTGTCAAGGCCCATGTTTCTGTATGTGAGAACCAATGGGACCTCCAAGATCAAGATGAAATGGGGTATGGAAATGAGACGCTGCCTTCTTCAATCTCTCCAACAGATTGAGAGCATGATTGAAGCTGAATCCTCCGTCAAAGAGAAAGACCTGACCAAAGAATTCTTTGAAAACAAATCAGAAACATGGCCAATTGGAGAATCACCTAAGGGAGTGGAGGAAGGTTCCATCGGAAAGGTGTGCAGAACCTTACTAGCAAAATCTGTATTCAACAGCCTATATGCATCTCCACAACTCGAGGGGTTCTCAGCTGAATCGAGAAAACTGCTACTCATTGTTCAGGCGCTTAGGGATAACCTGGAACCTGGAACCTTCGATCTTGAAGGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTAAGATAG
ATGGAAGTAGTATCACTAATAACTATACTACTAGTAGTAACAGTAAGCAATGCAGATAAGATCTGCATCGGCTATCAATCAACAAACTCCACAGAAACTGTAGACACACTAACAGAAAACAATGTCCCTGTGACACATGCCAAAGAACTGCTCCACACAGAGCATAATGGGATGCTGTGTGCAACAAGCTTGGGACACCCTCTTATTCTAGACACCTGTACCATTGAAGGACTAATCTATGGCAATCCTTCTTGTGATCTATTGTTGGGAGGAAGAGAATGGTCCTATATCGTCGAGAGACCATCAGCTGTTAACGGATTGTGTTATCCCGGGAATGTAGAAAATCTAGAAGAGCTAAGGTCACTTTTTAGTTCTGCTAGGTCTTATCAAAGGATCCAGATTTTCCCAGACACAATCTGGAATGTGTCTTACAGTGGGACAAGCAAAGCATGTTCAGATTCATTCTACAGAAGCATGAGATGGTTGACTCAAAAGAACAACGCTTACCCTATTCAAGACGCCCAATACACAAATAATCAAGAAAAGAACATTCTTTTCATGTGGGGCATAAATCACCCACCCACCGATACTGCGCAGACAAATCTGTACACAAGAACCGACACAACAACGAGTGTGGCAACAGAAGAAATAAATAGGACCTTCAAACCATTGATAGGACCAAGGCCTCTTGTCAACGGTTTGATGGGAAGAATTGATTATTATTGGTCGGTATTGAAACCGGGTCAAACACTGCGAATAAGATCTAATGGGAATCTAATAGCTCCATGGTATGGACACATTCTTTCAGGAGAGAGCCACGGAAGAATCCTGAAGACTGATTTAAAAAGGGGTAGCTGCACAGTGCAATGTCAGACAGAGAAAGGTGGCTTAAACACAACATTGCCATTCCAAAATGTAAGTAAGTATGCATTTGGAAACTGCTCGAAATACATTGGCATAAAGAGTCTCAAACTTGCAGTTGGTCTGAGGAATGTGCCTTCTAGATCTAGTAGAGGACTATTCGGGGCCATAGCAGGATTCATAGAGGGAGGTTGGTCAGGACTAGTTGCTGGTTGGTATGGATTCCAGCATTCAAATGACCAAGGGGTTGGTATGGCAGCAGATAGAGACTCAACCCAAAAGGCAATTGATAAAATAACATCCAAAGTGAATAACATAGTCGACAAAATGAACAAGCAGTATGAAATTATTGATCATGAATTCAGCGAGGTTGAAACTAGACTTAACATGATCAATAATAAGATTGATGATCAAATCCAAGATATATGGGCATATAATGCAGAATTGCTAGTTCTGCTTGAAAACCAGAAAACACTCGATGAGCATGATGCAAATGTAAACAATCTATATAATAAAGTGAAGAGGGCGTTGGGTTCCAATGCGGTGGAAGATGGGAAAGGATGTTTCGAGCTATACCACAAATGTGATGACCAGTGCATGGAGACAATTCGGAACGGGACCTACAACAGAAGGAAGTATCAAGAGGAGTCAAAATTAGAAAGACAGAAAATAGAGGGGGTCAAGCTGGAATCTGAAGGAACTTACAAAATCCTCACCATTTATTCGACTGTCGCCTCATCTCTTGTGATTGCAATGGGGTTTGCTGCCTTCTTGTTCTGGGCCATGTCCAATGGGTCTTGCAGATGCAACATTTGTATATAA
ATGGCGTCTCAAGGCACCAAACGATCCTATGAACAGATGGAAACTGGTGGGGAACGCCAGAATGCTACTGAGATCAGGGCATCTGTTGGAAGAATGGTTAGTGGCATTGGGAGATTCTACATACAGATGTGTACAGAACTCAAACTCAGTGACAATGAAGGGAGGCTGATTCAGAACAGTATAACAATAGAGAGAATGGTACTCTCTGCATTTGATGAAAGAAGGAACAGATACCTGGAAGAGCACCCCAGTGCAGGAAAGGACCCTAAGAAAACTGGAGGTCCAATTTACAGGAGAAGAGACGGGAAATGGGTGAGAGAGCTGATCCTGTATGACAAAGAGGAAATCAGGAGAATTTGGCGACAAGCGAACAATGGAGAGGATGCAACTGCTGGTCTTACCCATCTGATGATATGGCATTCCAACCTGAATGATGCCACCTATCAGAGAACGAGAGCTCTCGTGCGTACTGGAATGGATCCCCGGATGTGCTCTCTGATGCAAGGATCAACTCTTCCGAGGAGATCTGGAGCTGCAGGTGCAGCAGTAAAGGGGATAGGGACAATGGTGATGGAACTGATTCGGATGATAAAACGAGGGATCAACGACCGGAATTTCTGGAGAGGCGAAAATGGAAGAAGGACAAGGATTGCATACGAGAGGATGTGCAACATCCTCAAAGGGAAATTCCAAACAGCAGCACAAAGGGCAATGATGGATCAAGTGCGAGAGAGCAGAAATCCTGGGAATGCTGAAATAGAAGATCTCATTTTTCTGGCAAGGTCTGCACTCATCCTGAGAGGATCAGTGGCTCATAAGTCCTGCTTGCCTGCTTGTGTGTACGGACTTGCAGTGGCCAGTGGATATGACTTTGAGAGAGAAGGGTACTCCCTGGTTGGAATAGATCCTTTCCGTCTGCTTCAAAACAGCCAGGTCTTTAGTCTCATTAGACCAAATGAGAACCCAGCACATAAGAGCCAATTAGTGTGGATGGCATGCCACTCTGCAGCATTTGAGGACCTTAGGGTCTCAAGTTTCATTAGAGGGACAAGAATGGTCCCAAGAGGACAGCTATCCACTAGAGGGGTTCAAATTGCTTCAAATGAGAACATAGAAGCAATGGACTCCAATACTCTTGAACTGAGAAGTAGATATTGGGCTATAAGAACCAGAAGCGGAGGAAACACCAACCAACAGAGGGCATCTGCAGGACAGGTCAGCGTTCAACCCACTTTCTCAGTACAGAGAAATCTTCCCTTCGAAAGAGCAACCATTATGGCAGCATTTACAGGAAATACTGAGGGTAGAACGTCTGACATGAGGACTGAAATCATAAGAATGATGGAAAGTGCCAGACCAGAAGATGTGTCATTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAAGGCAACGAACCCGATCGTGCCTTCCTTTGACATGAATAATGAAGGATCTTATTTCTTCGGAGACAATGCAGAGGAGTATGACAATTAA
ATGAACCCAAATCAGAAGATAATAGCAATTGGCTCTGTTTCTCTAACCATTGCGATAATATGTTTTCTCATGCAGATTGCCATCTTAACAACGACCATGACACTACATTTCAGGCAGAATGAATGCAGCAATCCATCGAATAATCAAGTGGTGCCATGTGAACCTATCATAATAGAGAAGAACACAGTGCATTTGAATAGTACTACCATAGAGAAGGAAATTTGTCCTAAAGTGGCAGAATACAAGAATTGGTCAAAACCACAATGTCAAATTACAGGGTTCGCTCCTTTCTCAAAGGACAACTCAATTAGGCTTTCTGCAGGTGGAGATATCTGGGTGACAAGAGAACCTTATGTGTCGTGCAGTCTTGGCAAATGTTATCAATTTGCACTTGGGCAGGGAACCACTCTGAAAAACAAGCACTCAAATGGCACTACACATGATAGAATTCCTCACAGAACTCTCTTAATGAATGAGTTAGGTGTCCCATTTCATTTGGGAACCAAACAAGTGTGCATAGCATGGTCTAGTTCAAGCTGCCATGATGGGAAAGCATGGTTACATATTTGTGTGACTGGGGATGATAAAAATGCTACTGCTAGTATCATTTATGATGGGATGCTGGCTGACAGTATTGGTTCATGGTCCAAAAACATCCTAAGAACCCAGGAGTCAGAATGCGTTTGCATCAATGGAACTTGTGCAGTGGTAATGACTGATGGAAGTGCATCAGGAAAGGCTGACACTAGAATATTATTCATAAGAGAGGGGAAAATTATAAATATTAGCCCATTGTCAGGAAGTGCTCAGCATGTGGAGGAATGTTCCTGTTACCCCCGGTATCCTGAAGTTAGGTGTGTTTGCAGAGACAACTGGAAGGGCTCCAATAGGCCCGTTCTATATATAAATATGGCAGATTATAGTATTGAGTCCAGTTATGTATGCTCAGGACTTGTTGGCGACACACCAAGAGATGATGATAGCTCCAGCAGCAGTAACTGTAGAGACCCTAATAACGAAAGAGGGGCCCCAGGAGTGAAAGGGTGGGCCTTTGACGATGGGAATGACATTTGGATGGGACGAACAATCAAAAGTGATTCACGCTCAGGTTATGAGACTTTTAGGGTCGTTAATGGTTGGACCACGGCTAATTCCAAGTCACAGGTAAATAGGCAAGTCATAGTTGACAGTGACAGCTGGTCTGGGTATTCTGGTATCTTCTCTGTTGAAGGCAAGAACTGCATCAACAGGTGTTTTTATGTGGAGTTGATAAGAGGGAGACCACAGGAGACCAGAGTGTGGTGGACTTCAAACAGCATCATTGTATTCTGTGGAACCTCAGGTACATATGGAACAGGCTCATGGCCTGATGGAGCGAATATCGACTTCATGCCTATATAA
ATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCATTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAGGATGTTTTTGCAGGGAAGAACGCAGATCTCGAGGCTCTCATGGAGTGGATAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGGTTTGTCCAAAACGCCCTAAATGGGAATGGAGACCCAAACAACATGGACAAGGCAGTTAAATTATACAAGAAACTGAAGAGGGAAATGACATTTCATGGAGCAAAGGAAGTTGCACTCAGTTACTCAACTGGTGCGCTTGCCAGCTGCATGGGTCTCATATACAACAGGATGGGGACAGTAACCGCAGAAGGGGCTCTTGGACTAGTATGTGCCACTTGTGAGCAGATTGCTGACGCACAACATCGGTCCCACAGGCAGATGGCGACTACTACCAACCCACTAATTAGGCATGAGAATAGAATGGTACTAGCCAGCACTACGGCTAAGGCTATGGAGCAGATGGCTGGATCAAGTGAACAGGCAGCGGAAGCCATGGAAGTCGCAAGTCAGGCTAGGCAAATGGTGCAGGCTATGAGAACAGTCGGGACTCACCCTAACTCCAGTACAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGGCTTACCAGAACCGGATGGGAGTGCAACTGCAGCGGTTCAAGTGATCCTCTCGTTGTTGCAGCTAACATTATTGGGATATTGCACTTGATATTGTGGATTCTTGATCGTCTTTTCTTCAAATGCATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAATGCCTGAGTCTATGAGGGAAGAATATCGGCAGGAACAGCAGAATGCTGTGGATGTTGACGATGGTCATTTTGTCAACATAGAGCTG-AGTAA
ATGGATTCCAACACTGTGTCAAGCTTCCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATTTGCAGACCAAGAACTGGGTGATGCCCCATTTCTAGACCGGCTTCGCCGAGATCAGAAGTCCCTGAGAGGAAGAAGCAGCACTCTTGGTCTGGACATCAGAACTGCTACTCGTGAAGGAAAGCATATAGTGGAACGGATTTTAGAGGACGAGTCAGATGAAGCATTTAAAATGAGTATTGCTTCAGTGCCAGCTCCACGCTATCTAACTGACATGACTCTTGAAGAAATGTCAAGAGATTGGTTAATGCTCATTCCCAAACAGAAAGTGACAGGGTCCCTTTGCATTAGAATGGACCAAGCAATAGTGGACAAAAACATCACATTGAAAGCAAATTTCAGTGTGATTTTCAATCGACTTGAAGCCCTGATACTACTTAGAGCTTTTACGGAAGAAGGAGCAATTGTGGGCGAAATCTCACCATTACCTTCTCTTCCAGGACATACTGACAAGGATGTCAAAAATGCAATTGAGGTCCTCATCGGAGGATTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAAGCAGCGATGAGGATGGGAGACCTCCACTCTCTACAAAGTAGAAACGGGAAATGGAGAGAACAGCTAAGCCAGAAGTTCGAAGAAATAAGATGGTTGATTGAAGAAGTACGACATAGATTAAAGATTACGGAGAATAGCTTTGAGCAAATAACTTTTATGCAAGCCTTACAACTATTGCTTGAAGTGGAGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAA

d(i_bird)=(5,12,16,-,10,-,27,31)


-----------update 25.May.2013 , new segment 7 published from March,April from Nanjing,Taiwan,Shanghai------

>A/Index_poultry_East-China/2008//,H9N2
ATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCATTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAGGATGTTTTTGCAGGGAAGAACACAGATCTTGAGGCTCTCATGGAATGGATAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGGTTTGTCCAAAACGCCCTAAATGGGAATGGAGACCCAAACAACATGGACAGGGCAGTTAAATTATACAAGAAGCTGAAGAGGGAAATGACATTTCATGGAGCAAAGGAAGTTGCACTCAGTTACTCAACTGGTGCGCTTGCCAGCTGCATGGGTCTCATATACAACAGGATGGGAACAGTGACCGCAGAAGGGGCTCTTGGCCTAGTATGTGCCACTTGTGAGCAGATTGCTGACGCCCAACATCGGTCCCACAGGCAGATGGCGACTACTACCAACCCACTAATTAGGCATGAGAATAGAATGGTACTAGCCAGCACTACGGCTAAGGCTATGGAGCAGATGGCTGGATCAAGTGAACAGGCAGCAGAAGCCATGGAAGTCGCAAGTCAGGCTAGGCAAATGGTGCAGGCTATGAGAACAGTCGGGACTCACCCTAGCTCCAGTACAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGGCTTACCAGAAACGGATGGGAGTGCAACTGCAGCGATTCAAGTGATCCTCTCGTTGTTGCAGCAAGCATTATTGGGATATTGCACTTGATATTGTGGATTCTTGATCGTCTTTTCTTCAAATGCATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAGTGCCTGAGTCTATGAGGGAAGAATATCGGCAGGAACAGCAGAATGCTGTGGATGTTGACGATGGTCATTTTGTCAACATAGAGCTGGAGTAA

d=25,28

[gsgs]

Re: H7n9

genbank gets HA,NA,MP from the Hangzhou case
It's good to see that genbank also gets some of the sequences.

They are not yet available through the "front door" (searchable)
It looks similar to S2(Shanghai/2) and A1(Anhui/1),
NP would be interesting to date the NP-reassortment

http://www.ncbi.nlm.nih.gov/nuccore/KC853764

http://www.ncbi.nlm.nih.gov/nuccore/KC853765

http://www.ncbi.nlm.nih.gov/nuccore/KC853766

[gsgs]

Re: Is H7N9 Spreading from Human to Human in China?

> The N9 gene was identical to a virus found in wild birds from South Korea.

I will believe it when I see the sequence at genbank

---------------------------------------------------

http://www.newfluwiki2.com/diary/1591/

08/19/2007,Nebraska,poultry farm near Seward,turkeys,H7N9 low path,
meaning it was not a threat to human health ... Steffen said
---------------
to low- pathogenicity H7N9 was detected in US flocks in 2007, 2009, and 2011. "I believe
chickens and turkeys in Nebraska, Minnesota, Illinois, Kentucky,
-----------------------------------------

[gsgs]

risk statements

Frieden:
> Because few people spend time around live chickens, it is unlikely to develop into a full-blown pandemic.
> we have a system in place that's more prepared than ever to respond to emergencies

Cox:
> In some ways, H7N9 is more worrisome than H1N1 was

One scientist:
> the sequence raised concern about a potential global epidemic but it was impossible
> to give a precise estimate of the likelihood

Webby:
> At this stage it's still unlikely to become a pandemic

Garcia Sastre:
> Most likely, the main sources of the virus are chickens and duck farms
> At this moment it’s very unclear how much of a danger the virus poses,
> The flu is very unpredictable.
> I don’t think it’s a threat unless it starts spreading from human to human,
> which I think is unlikely. We need to know a little more about the epidemiology
> of the virus, but I really don’t think it’s a big threat.

reporter:
> and public health officials are worried it could become a worldwide pandemic

WHO:
> a pandemic is unlikely, given that there is no evidence of person-to-person transmission of the virus.
Hartl:
> The source of contagion could be contact with birds or mere proximity to them

Garrett:
> We need to know how are these people getting infected.Who are they getting it from?
> We need to know what's the denominator. How many people out there in China are
> infected right now with this virus harmlessly or with very mild illness? Third, we need
> to know what species did this come from. Did it come from birds? Did it come from dead pigs?
> Did it come from other animals of some kind? Until we have those three big questions answered,
> we have no capacity to speculate about the probability that this will become the next great pandemic.

it seems that soon these questions will be answered, Will she speculate about the probability then
or will she find another excuse ? I'm at 80% for the latter currently.

http://biosurveillance.typepad.com/

Ascel Bio:
> NCoV Presently "Bigger Concern" Than H7N9
Ascel Bio's analysis is based on official disclosures and other open source reports.
Ascel Bio is monitoring over 200 seasonal diseases in over 17 countries on behalf of our customers
since 2010 www.ascelbio.com

CDC:
as CDC scientists stepped up efforts to determine its pandemic risk.
There is no evidence that the H7N9 bird flu

Cox:
> I'd have to say that it's very similar to the level of concern for H5N1 in many respects.
> H5N1 viruses have a very wide geographic spread, while that's not known to be the case with H7N9,
> But on the other hand, , the fact that we're hearing about an additional two to maybe five cases
> per day in China sort of raises the level of concern in the opposite direction in favor of H7N9.

Derek Smith:
> If what we know about these mutations in other flu subtypes is also the case for this virus,
> then it's already part way there,That's why everybody is concerned about this.

Fouchier:
> All pandemic viruses have acquired this [mammalian cell] binding and polymerase activity,
> The H7N9 has these characteristics. So the question is, what else does it need – if anything?

Garcia-Sastre
> while it is too early to be able to conclude anything … the probabilities are very low
> that a global pandemic is looming.
> I am comforted by the lack of a surge in the numbers of people with the disease.
> It’s not that it’s increasing by ten times per week, I think right now the number of cases
> is what you would have expected from the original numbers
> Right now there are no major indications to become highly alarmed.

Oxford
> the situation in China is getting a little more worrying.
> I don’t like the sound of it. Every day I open up the reports and find out someone else has died,
> I just don’t like to see the figures going up day after day.
> So far there’s no human-to-human transmission. What’s tomorrow going to bring, what’s the
> next day going to bring? You don’t know and I don’t know,
> there is no need for anyone to start flapping at the moment.
> I don’t think we should start thinking of 1918 scenarios, definitely not

McCauley
> We’re watching very carefully the events there [in China] because we are aware although
> there’s no human-to-human transmission, these are unusual infections people have been
> getting from an avian reservoir,“China will need to identify the source and hopefully be able
> to control the cross-species transmission,We’re watching very carefully to see how it does.
> In the meantime, the national influenza centers around the world are developing their ability
> to detect this newly emerging virus” and also working on vaccines,
> Experts need to find out how vaccines would perform in case this virus becomes pandemic

Webby
> My gut feeling, at this point in time, is that this guy [H7N9] is probably closer to
> doing it, [h2h as compared to H5N1]

Cox
> the coming weeks may tell whether H7N9 is only a bird disease that occasionally spills
> over into humans, the potential seed of a dangerous global flu pandemic, or something in between.

Haertl, 2013.04.16
> It is premature to dismiss the possibility of an #H7N9 pandemic or to say the outbreak is under control.

Lipsitch,2013.04.15
> It's too soon to say how big a threat H7N9 poses because we don't know how many animals
> of which species have it, how genetically diverse it is, or what the geographic extent is.
> It looks as though it will be at least as challenging as H5N1
> If that pace keeps up or accelerates, H7N9 "could be a significant public health problem
> even if it remains an infection that people catch from animals

Farrar,2013.04.15
> I think we need to be very, very concerned about the latest developments,
> Perhaps counterintuitively, such mild cases are very worrying. That is because reduced virulence
> can often point to further genetic adaptation of the virus to infection of human beings —
> and thus greater potential to spread.

Xinhua,2013.04.16
> H7N9 under control


-----------------------------------------
statflu

http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19256

-----------------------------------------------------------
May,08,2013
Dr. Daniel Jernigan, deputy director of CDC's influenza division,
> people who get it [H7N9] usually get very sick,
Fauci:
> The pandemic-preparedness engines are already humming,
After all, health officials can't just kill every chicken in China.
There are lots of chickens in China. (~14B)

---------------------------------------------------------
June,01,2013 , Liu W, Zhu Y, Qi X, Xu K, Ge A, Ji H, Ai J, Bao C, Tang F, Zhou M. write:
> Briefly, it is highly unlikely that a pandemic of human infection with avian influenza A (H7N9)
> Avirus will happen in Jiangsu Province in the near future.

http://www.ncbi.nlm.nih.gov/pubmed/23720670

----------------------------------------------------------------

[gsgs]

Re: H7n9

onset2.txt : Sharon's list in computer-form

60 cases by 10-year-age-groups 0-4,5-14,15-24,etc.
60 cases by (estimated) onset-day
weekly cases by onset date
weekly hospitalisations




(to be updated)



here are better and bigger charts:

http://www.uq.edu.au/vdu/VDUInfluenza_H7N9.htm

http://www.cidrap.umn.edu/cidrap/files/78/mceirs_h7n9_graphs.pdf

-----------------------------------------
we observe that elderly individuals have strongest resistance to IVA partly by
presenting more terminal α2-3/6-linked sialic acid residues in their saliva,
which bind with the influenza viral hemagglutinations
------------------------------------------

[sharon sanders]

Re: H7n9

Nice! Thanks!

[gsgs]

Re: H7n9

they have a full genome at genbank today:
A/Zheijiang/DTID-ZJU01/2013/04/03(H7N9) , male , Apr.3, severe case

627E(1),HA identical to Anhui/1,PA reassorted
(MRCAD ~2010/08 from public H9N2)

which of our cases is that

-----------------------
Apr.3:
The Zhejiang Provincial Health Department said laboratory tests have confirmed that a 38-year-old cook
who died last month was suffering from avian influenza, which is better known as bird flu.
The tests showed he had contracted H7N9, which is a relatively new strain that was only known to infect birds.
Authorities said the man, who worked in neighboring Jiangsu Province, became ill on March 7,
was hospitalized on March 18, and died on March 27. A second person, a 67-year-old retired man
from Zhejiang province, has also tested positive for H7N9 after becoming ill on March 25 with fever,
cough and other symptoms. He was later hospitalized but his condition was not immediately known.

[gsgs]

Re: H7n9

A/chicken/Zhejiang/DTID-ZJU01/2013(H7N9) at genbank, call this Z2

fecal samples, Apr-2013

Z2 has 21 differences in HA and 11 in NA from S1
that makes me change my estimate of the first
H9N2+H7N9 reassortment to ~late 2011, 1-2 years ago
------edit----- but 10 of these mutations are concentrated at 108-321
and 6 are nonsynonymous, strange. So cancel my 1-2 year estimate again
we may be seeing strong selection in HA
that could mean frequent exchange between 2,3 and 2,6 hosts (speculation)
------------

KC899666-KC899673

http://www.ncbi.nlm.nih.gov/nuccore/KC899666

http://www.ncbi.nlm.nih.gov/nuccore/KC899667

http://www.ncbi.nlm.nih.gov/nuccore/KC899668

http://www.ncbi.nlm.nih.gov/nuccore/KC899669

http://www.ncbi.nlm.nih.gov/nuccore/KC899670

http://www.ncbi.nlm.nih.gov/nuccore/KC899671

http://www.ncbi.nlm.nih.gov/nuccore/KC899672

http://www.ncbi.nlm.nih.gov/nuccore/KC899673

[gsgs]

Re: H7n9

when we remove the segments that are obviously reassorted
subsequently or look fishy, then the diversity is very low.
Much lower than what we saw with ****** after only
~3 months of evolution in end April 2009.
I remember we had a few sequences in Mexico that had
only 2-4 mutations in the whole genome as late as mid April
while the others had already 10-20 mutations.
We never found a good explanation for this.

In the list below I removed segments 2,4,5,6 from S1,3 from Z1, 4 from Z2.
1,2,3,5,8 from H1 were not available to me.
Maybe someone with access to gisaid can complete the list.
Also with the 3 missing nonhuman genomes.
I removed the position numbers and only used "X" for different nucleotides,
so the list should be gisaid-secrecy-compatible.

Index/2013_79,5                               ............ .... .X.X .... . ........ ..... .... 
A/Shanghai/1/2013,H7N9                        XXXX..XXX... ---- .XXX ---- - -------- .X... .XX. 
A/Anhui/1/2013,H7N9                           ............ .X.. X... .... . ...X.... ..... .... 
A/Shanghai/2/2013,H7N9                        .....X...X.. X... .... ..X. . X....... ..... .... 
A/Hangzhou/1/2013/03/24,H7N9                  ------------ ---- ---- X..X - .X...... ...XX ---- 
A/Zhejiang/DTID-ZJU01/2013,2013//,H7N9        ......X...XX ...X ---- .... X ....X.XX X.... ...X 
A/Ck/Zhejiang/DTID-ZJU01/2013,2013/04/,H7N9   ....X.....X. ..X. .... .X.. . ..X..XX. ..X.. X...

differences from the index

|12345678|
--+--------+
S1|7-3---12|
A1|01100100|
S2|21010100|
H1|---2-12-|
Z1|31--1311|
Z2|21010311|
--+--------+

with an average of 2.8,1.0,1.0,1.0,0.3,1.8,.8,0.8
mutations away from the index in the 8 segments or
9.5 mutations in the whole genome. That gives a dating
of the index 3.6 months ago, well compatible with the
Chinese New Year movements.

The H7N9 reassortment may well have happened earlier,
but then there was a bottleneck ~3 months ago, and the
currently circulating H7N7 almost all descends from
that one bottleneck virus in all segments.


This is different from my first analysis, when only
S1,A1,S2 were available and I assumed reassortments.
In this new scenario/hypothesis the many mutations
in HA and NA would be due to selection pressure
or sequencing errors or prolonged disease in S1
and the initial H7N7 reassortment only happened
early this year.

We will soon get more reliable estimates when new
sequences come in. Hopefully also some H9N2 poultry
sequences, that show the subsequent reassortments.

[gsgs]

Re: H7n9

here is my H7N9 index-virus, the one that I think made
the initial H9N2+H7N9-->H7N9 reassortment in early 2013

(or bottleneck/superspreader virus or subsequent reassortment virus,
while earlier viruses died or were not found yet)

>A/Index/H7N9-China/2013/03/,H7N9
ATGGAAAGAATAAAAGAACTAAGAGATTTGATGTCACAGTCTCGCACTCGCGAGATACTGACAAAAACAACTGTGGACCATATGGCCATAATCAAGAAATATACATCAGGAAGACAGGAGAAGAATCCTGCCCTTAGGATGAAGTGGATGATGGCAATGAAATATCCAATTACGGCAGACAAAAGGATAATGGAGATGATCCCGGAAAGAAATGAGCAAGGTCAGACCCTTTGGAGCAAGACAAATGATGCTGGATCAGACAGAGTGATGGTGTCACCTCTGGCTGTGACGTGGTGGAACAGAAATGGACCAACGACAAGCACAGTCCATTATCCAAAGGTCTATAAAACCTATTTTGAAAAGGTCGAAAGGCTAAAACATGGAACCTTCGGCCCCGTTCACTTCAGAAACCAGGTTAAAATACGCCGCAGGGTCGACATAAACCCGGGCCATGCAGATCTTAGTGCTAAAGAAGCACAGGATGTCATCATGGAGGTCGTATTCCCAAACGAAGTTGGAGCCAGAATATTGACATCAGAGTCACAGTTAACGATTACCAAGGAAAAGAAGAAGGAGCTTCAGGACTGCAAAATTGCCCCTTTAATGGTGGCTTACATGTTGGAGAGAGAACTGGTTCGCAAAACAAGATTCCTACCAGTAGCTGGAGGGACAAGCAGCGTGTATATCGAGGTGTTGCATTTGACCCAAGGGACCTGCTGGGAGCAAATGTACACACCGGGAGGGGAAGTGAGAAATGATGATGTTGATCAGAGTTTAATTATTGCTGCTAGAAATATTGTTAGAAGAGCAACAGTATCAGCAGACCCGTTGGCTTCGCTTTTGGAGATGTGCCATAGTACACAGATTGGCGGGGTTAGGATGGTTGACATCCTTAGACAAAACCCAACAGAAGAACAGGCTGTGGATATATGTAAGGCAGCAATGGGTCTAAGGATCAGTTCATCCTTCAGCTTTGGAGGTTTCACTTTCAAAAGGACAAGTGGGTCATCTGTCAAAAGGGAAGAAGAAGTGCTCACAGGCAACCTCCAAACATTGAAAATAAGAGTACATGAAGGATATGAGGAATTCACAATGGTCGGGCGAAGAGCAACAGCCATTCTAAGGAAAGCAACCAGAAGACTGATCCAACTGATAGTGAGTGGGAAAGACGAGCAATCAATCGCCGAGGCAATCATAGTGGCAATGGTATTCTCACAAGAGGATTGTATGATAAAGGCAGTGAGAGGTGATTTGAACTTTGTCAACAGAGCAAACCAGCGGCTAAATCCCATGCATCAACTCCTGAGGCATTTCCAAAAGGATGCAAAGGTCCTGTTTCAAAACTGGGGAATTGAACCCATTGACAATGTAATGGGGATGATCGGAATATTGCCTGACATGACCCCCAGCACAGAGATGTCATTGAGAGGAGTGAGAGTTAGTAAAATGGGAGTAGATGAATATTCCAGCACTGAGAGAGTGGTCGTGAGTATTGATCGTTTCTTGAGGGTCCGAGACCAGAGGGGAAACGTACTCCTGTCTCCTGAAGAGGTTAGTGAAACACAGGGAACAGAAAAGCTGACTATAACATATTCATCGTCCATGATGTGGGAGATCAATGGTCCGGAATCAGTGCTAGTTAACACATATCAATGGATCATTAGAAATTGGGAAAATGTAAAGATTCAATGGTCCCAAGATCCTACAATGCTATACAATAAGATGGAATTTGAACCCTTTCAATCCCTAGTGCCTAAAGCTGCCAGAGGCCAATATAGTGGGTTCGTGAGGGTTCTATTCCAACAGATGCGTGACGTACTGGGAACATTTGACACTGTCCAAATAATAAAGCTATTACCATTTGCAGCAGCCCCGCCGGAGCAGAGTAGGATGCAGTTCTCTTCTCTAACTGTGAATGTGAGGGGTTCCGGAATGAGAATAGTTGTGAGAGGCAATTCTCCTGTGTTCAACTACAACAAGGCAACCAAGAGGCTTACGGTGCTTGGAAAGGATGCAGGTGCATTGATGGAAGACCCCGATGAGGGAACAGCAGGAGTGGAATCTGCGGTATTGAGGGGATTTCTGATTCTGGGCAAAGAAGACAAAAGATATGGGCCAGCATTGAGCATCAACGAATTGAGCAATCTTGCGAAAGGAGAGAAGGCTAATGTGTTGATAGGGCAAGGAGACGTTGTGTTGGTGATGAAACGGAAACGGGACTCTAGCATACTTACTGACAGTCAGACAGCGACCAAAAGGATTCGGATGGCCATCAATTAA
ATGGATGTCAATCCGACTTTACTTTTCTTGAAAGTGCCAGTGCAAAATGCTATAAGTACCACTTTCCCTTATACTGGAGACCCTCCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATAAATACTCAGAAAAAGGAAAGTGGACAACGAACACAGAGACTGGAGCACCCCAACTCAATCCAATTGATGGACCATTACCTGAGGACAACGAGCCGAGTGGGTATGCACAAACGGATTGTGTATTGGAAGCAATGGCTTTCCTTGAAGAATCTCACCCAGGGATCTTTGAAAACTCGTGTCTCGAAACGATGGAAATTGTTCAGCAAACAAGAGTGGATAAACTGACCCAAGGCCGCCAGACCTATGACTGGACGTTGAATAGAAATCAGCCGGCTGCTACCGCATTGGCCAACACTATAGAGGTATTCAGATCGAATGGCCTGACAGCCAATGAATCAGGAAGGTTGATCGATTTCCTCAAGGACGTGATGGATTCAATGGATAAGGAAGAAATGGAGATTACAACACATTTCCAGAGGAAGAGGAGAGTGAGGGACAACATGACCAAGAAAATGGTCACACAGAGAACAATAGGAAAGAAAAAACAAAGACTGAACAAAAGGAGCTACCTAATAAGAGCACTGACATTGAACACAATGACAAAGGATGCTGAAAGAGGCAAGCTGAAAAGGAGGGCAATCGCAACACCCGGGATGCAAATCAGAGGATTCGTGTATTTTGTAGAAGCACTAGCGAGGAGCATCTGTGAGAAACTTGAGCAATCTGGCCTCCCTGTCGGAGGGAATGAGAAGAAAGCTAAATTGGCAAATGTTGTGAGGAAGATGATGACTAATTCACAAGATACAGAGCTCTCCTTCACAATTACTGGGGACAACACCAAATGGAATGAGAATCAAAACCCCCGGATGTTTCTAGCAATGATAACATACATCACAAGAAACCAGCCAGAATGGTTTAGAAATGTCTTAAGCATTGCTCCTATAATGTTCTCAAACAAGATGGCGAGATTAGGAAAAGGGTACATGTTCGAAAGTAAGAGTATGAAGTTACGGACACAAGTACCAGCGGAAATGCTCGCAAATATTGACCTGAAATACTTCAACAAATCAACAAGAGAGAAAATCGAGAAAATAAGACCTCTACTGATAGATGGCACAGCCTCATTGAGTCCTGGAATGATGATGGGCATGTTCAACATGTTGAGTACAGTCTTAGGAGTTTCAATTCTGAATCTCGGGCAGAAGAAGTACACCAAAACCACATATTGGTGGGACGGACTCCAATCCTCAGATGACTTCGCCCTCATAGTGAATGCACCGAATCATGAGGGAATACAGGCAGGAGTAGATAGGTTCTATAGAACCTGCAAATTAGTTGGGATAAACATGAGCAAGAAGAAATCCTACATAAATCGGACAGGAACATTCGAATTCACAAGCTTTTTCTACCGCTATGGATTCGTAGCTAACTTCAGTATGGAGTTGCCCAGTTTTGGAGTGTCCGGGATTAATGAGTCAGCTGACATGAGCGTTGGTGTTACAGTAATAAAGAACAATATGATAAACAACGATCTTGGACCAGCAACAGCCCAAATGGCCCTTCAGCTATTTATCAAAGACTACAGATACACATACCGATGTCACAGGGGTGATACGCAAATTCAAACGAGGAGAGCATTCGAGCTGAAGAAGCTGTGGGAGCAGACCCGTTCGAAGGCAGGACTGTTGGTTTCAGATGGAGGGCCAAACCTGTACAATATCCGGAACCTCCACATTCCAGAGGTCTGCTTGAAATGGGAATTGATGGATGAAGACTACCAAGGCAGGTTGTGTAATCCTATGAACCCGTTTGTCAGTCATAAGGAAATTGATTCAGTCAACAATGCTGTGGTGATGCCAGCTCATGGCCCAGCCAAAAGCATGGAGTATGATGCCGTTGCAACCACACATTCATGGATTCCTAAGAGGAATCGCTCCATTCTCAACACCAGCCAAAGGGGGATTCTTGAGGACGAACAGATGTACCAGAAGTGCTGCAACCTATTCGAAAAGTTCTTCCCCAGCAGTTCGTACAGGAGGCCAGTTGGAATTTCCAGCATGGTGGAGGCCATGGTGTCTAGGGCCCGAATTGATGCACGAATTGACTTCGAATCTGGAAGGATTAAGAAAGAAGAGTTTGCTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCTCAGACGGCAAAAATAG
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACAAACAAATTCGCATCAATATGCACACACTTAGAAGTCTGCTTCATGTACTCTGATTTCCACTTCATCGACGAACGAGGCGAATCAACTATAATAGAATCTGGCGATCCAAATGCGCTGCTGAAACACCGATTTGAAATAATCGAAGGGAGAGACCGAACAATGGCCTGGACAGTGGTGAATAGTATCTGCAACACCACAGGAGCCGAAAAACCCAAATTTCTCCCGGATCTGTATGACTACAAGGAAAACCGTTTCATTGAAATTGGAGTGACGAGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAATAAAATAAAATCCGAGAAGACACACATCCATATTTTTTCATTCACTGGAGAAGAGATGGCCACCAAAGCAGATTACACTCTTGACGAAGAAAGCAGGGCAAGAATCAAAACCAGGCTGTTCACCATAAGGCAGGAAATGGCCAGCAGGGGTCTATGGGATTCCTTTCGTCAGTCTGAAAGAGGCGAAGAAACAATTGAAGAAAGATTTGAAATCACAGGAACCATGCGCAGGCTTGCCGACCAAAGTCTCCCACCGAACTTCTCCAGCCTTGAAAACTTTAGAGCCTATGTGGATGGATTCGAACCGAACGGCTGCATTGAGGGCAAGCTTTCTCAGATGTCAAAAGAAGTGAACGCCAGAATTGAGCCATTTCTAAGAACAACACCACGCCCTCTCAGATTGCCTAATGGGCCTCCCTGCTCTCAGCGGTCGAAATTCTTGCTGATGGATGCTCTGAAATTAAGCATTGAGGACCCGAGCCACGAAGGGGAGGGGATACCGCTATATGATGCGATCAAATGCATGAAAACGTTCTTCGGGTGGAAAGAGCCCAACATTATCAAACCACATGAGAAAGGCATAAACCCCAATTATCTCCTGACTTGGAAGCAGGTGCTAGCAGAACTTCAGGACATTGAAAATGAAGAGAAGATTCCAAGGACAAAGAACATGAAGAAAACAAGCCAATTAAAGTGGGCACTCGGTGAGAACATGGCACCGGAGAAGGTGGACTTTGAGGATTGCAAAGATGTCAACGACTTGAAACAGTACAACAGTGATGAGCCAGAGCCCAGATCACTAGCATGTTGGATCCAGAATGAATTCAACAAGGCGTGTGAACTGACTGACTCAAGCTGGGTAGAACTTGATGAAATAGGGGAAGATGTTGCCCCAATCGAACACATTGCAAGCATGAGACGGAACTATTTTACAGCAGAGGTGTCCCACTGCAGGGCTACTGAATATATAATGAAGGGAGTGTACATAAATACAGCTTTGCTCAATGCATCTTGTGCAGCCATGGATGACTTTCAACTGATTCCAATGATAAGTAAATGTAGAACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGATTCATTATAAAAGGAAGATCTCATTTGAGGAATGATACCGACGTGGTAAACTTTGTAAGTATGGAATTTTCCCTTACCGACCCAAGGTTGGAACCACATAAATGGGAAAAGTATTGTGTTCTTGAAATAGGGGACATGCTCCTGCGAACTGCAGTAGGCCAAGTGTCAAGACCCATGTTTCTGTATGTGAGAACCAATGGGACCTCCAAGATCAAGATGAAATGGGGTATGGAAATGAGACGCTGCCTTCTTCAATCTCTCCAACAGATTGAGAGCATGATTGAAGCTGAATCCTCCGTCAAAGAGAAAGACCTGACCAAAGAATTCTTTGAAAACAAATCAGAAACATGGCCAATTGGAGAGTCACCTAAAGGAGTGGAGGAAGGTTCCATCGGGAAGGTGTGCAGAACCTTACTAGCAAAATCTGTATTCAACAGCCTATATGCATCTCCGCAACTCGAGGGGTTCTCAGCTGAATCGAGAAAACTGCTACTCATTGTTCAGGCGCTTAGGGATAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCTCACACATGCACTAAGATAG
ATGAACACTCAAATCCTGGTATTCGCTCTGATTGCGATCATTCCAACAAATGCAGACAAAATCTGCCTCGGACATCATGCCGTGTCAAACGGAACCAAAGTAAACACATTAACTGAAAGAGGAGTGGAAGTCGTCAATGCAACTGAAACAGTGGAACGAACAAACATCCCCAGGATCTGCTCAAAAGGGAAAAGGACAGTTGACCTCGGTCAATGTGGACTCCTGGGGACAATCACTGGACCACCTCAATGTGACCAATTCCTAGAATTTTCAGCCGATTTAATTATTGAGAGGCGAGAAGGAAGTGATGTCTGTTATCCTGGGAAATTCGTGAATGAAGAAGCTCTGAGGCAAATTCTCAGAGAATCAGGCGGAATTGACAAGGAAGCAATGGGATTCACATACAGTGGAATAAGAACTAATGGAGCAACCAGTGCATGTAGGAGATCAGGATCTTCATTCTATGCAGAAATGAAATGGCTCCTGTCAAACACAGATAATGCTGCATTCCCGCAGATGACTAAGTCATATAAAAATACAAGAAAAAGCCCAGCTCTAATAGTATGGGGGATCCATCATTCCGTATCAACTGCAGAGCAAACCAAGCTATATGGGAGTGGAAACAAACTGGTGACAGTTGGGAGTTCTAATTATCAACAATCTTTTGTACCGAGTCCAGGAGCGAGACCACAAGTTAATGGTCTATCTGGAAGAATTGACTTTCATTGGCTAATGCTAAATCCCAATGATACAGTCACTTTCAGTTTCAATGGGGCTTTCATAGCTCCAGACCGTGCAAGCTTCCTGAGAGGAAAATCTATGGGAATCCAGAGTGGAGTACAGGTTGATGCCAATTGTGAAGGGGACTGCTATCATAGTGGAGGGACAATAATAAGTAACTTGCCATTTCAGAACATAGATAGCAGGGCAGTTGGAAAATGTCCGAGATATGTTAAGCAAAGGAGTCTGCTGCTAGCAACAGGGATGAAGAATGTTCCTGAGATTCCAAAGGGAAGAGGCCTATTTGGTGCTATAGCGGGTTTCATTGAAAATGGATGGGAAGGCCTAATTGATGGTTGGTATGGTTTCAGACACCAGAATGCACAGGGAGAGGGAACTGCTGCAGATTACAAAAGCACTCAATCGGCAATTGATCAAATAACAGGAAAATTAAACCGGCTTATAGAAAAAACCAACCAACAATTTGAGTTGATAGACAATGAATTCAATGAGGTAGAGAAGCAAATCGGTAATGTGATAAATTGGACCAGAGATTCTATAACAGAAGTGTGGTCATACAATGCTGAACTCTTGGTAGCAATGGAGAACCAGCATACAATTGATCTGGCTGATTCAGAAATGGACAAACTGTACGAACGAGTGAAAAGACAGCTGAGAGAGAATGCTGAAGAAGATGGCACTGGTTGCTTTGAAATATTTCACAAGTGTGATGATGACTGTATGGCCAGTATTAGAAATAACACCTATGATCACAGCAAATACAGGGAAGAGGCAATGCAAAATAGAATACAGATTGACCCAGTCAAACTAAGCAGCGGCTACAAAGATGTGATACTTTGGTTTAGCTTCGGGGCATCATGTTTCATACTTCTAGCCATTGTAATGGGCCTTGTCTTCATATGTGTAAAGAATGGAAACATGCGGTGCACTATTTGTATATAA
ATGGCGTCTCAAGGCACCAAACGATCCTATGAACAGATGGAAACTGGTGGGGAACGCCAGAATGCTACTGAGATCAGGGCATCTGTTGGAAGAATGGTTAGCGGCATTGGGAGATTCTACATACAGATGTGTACAGAACTCAAACTCAGTGACAATGAAGGGAGGCTGATTCAGAACAGTATAACAATAGAGAGAATGGTACTCTCTGCATTTGATGAAAGAAGGAACAGATACCTGGAAGAGCACCCCAGTGCAGGAAAGGACCCTAAGAAAACTGGAGGTCCAATTTACAGGAGAAGAGACGGAAAATGGGTGAGAGAGCTGATCCTGTATGACAAAGAGGAAATCAGGAGAATTTGGCGACAAGCGAACAATGGAGAGGATGCAACTGCTGGTCTTACCCATCTGATGATATGGCATTCCAACCTGAATGATGCTACCTATCAGAGAACGAGAGCTCTCGTGCGTACTGGAATGGATCCCCGGATGTGCTCTCTGATGCAAGGATCAACTCTCCCGAGGAGATCTGGAGCTGCAGGTGCAGCAGTGAAGGGGATAGGGACAATGGTGATGGAACTGATTCGGATGATAAAACGAGGGATCAACGACCGGAATTTCTGGAGAGGCGAAAATGGAAGAAGGACAAGAATTGCATATGAGAGAATGTGCAACATCCTCAAAGGGAAATTCCAAACAGCAGCACAAAGGGCAATGATGGATCAAGTGCGAGAGAGCAGAAATCCTGGGAATGCTGAAATAGAAGATCTCATTTTTCTGGCAAGGTCTGCACTCATCCTGAGAGGATCAGTGGCTCATAAATCCTGCTTGCCTGCTTGTGTGTACGGACTTGCAGTGGCTAGTGGATATGACTTTGAGAGAGAAGGGTACTCCTTGGTTGGAATAGATCCTTTCCGTCTGCTTCAAAACAGCCAGGTCTTTAGTCTCATTAGACCAAATGAGAACCCAGCACATAAGAGCCAACTAGTGTGGATGGCATGCCACTCTGCAGCGTTTGAGGACCTTAGGGTCTCAAGTTTCATTAGAGGGACAAGAATGGTCCCAAGAGGACAGCTATCCACTAGAGGGGTTCAAATTGCTTCAAATGAGAACATGGAAGCAATGGACTCCAATACTCTTGAACTGAGAAGTAGATATTGGGCTATAAGAACCAGAAGCGGAGGGAACACCAACCAACAGAGGGCATCTGCAGGACAGGTCAGCGTTCAACCCACTTTCTCAGTACAGAGAAACCTTCCTTTCGAAAGAGCAACCATTATGGCAGCATTTACAGGAAATACTGAGGGTAGAACGTCTGACATGAGGACTGAAATCATAAGAATGATGGAAAGTGCCAGACCAGAAGATGTGTCATTCCAGGGGCGGGGAGTCTTCGAGCTCTCGGACGAAAAGGCAACGAACCCGATCGTGCCTTCCTTTGACATGAATAATGAAGGATCTTATTTCTTCGGAGACAATGCAGAGGAGTATGACAATTGA
ATGAATCCAAATCAGAAGATTCTATGCACTTCAGCCACTGCTATCATAATAGGCGCAATCGCAGTACTCATTGGAATAGCAAACCTAGGATTGAACATAGGACTGCATCTAAAACCGGGCTGCAATTGCTCACACTCACAACCTGAAACAACCAACACAAGCCAAACAATAATAAACAACTATTATAATGAAACAAACATCACCAACATCCAAATGGAAGAGAGAACAAGCAGGAATTTCAATAACTTAACTAAAGGGCTCTGTACTATAAATTCATGGCACATATATGGGAAAGACAATGCAGTAAGAATTGGAGAGAGCTCGGATGTTTTAGTCACAAGAGAACCCTATGTTTCATGCGACCCAGATGAATGCAGGTTCTATGCTCTCAGCCAAGGAACAACAATCAGAGGGAAACACTCAAACGGAACAATACACGATAGGTCCCAGTATCGCGCCCTGATAAGCTGGCCACTATCATCACCGCCCACAGTGTACAACAGCAGGGTGGAATGCATTGGGTGGTCAAGTACTAGTTGCCATGATGGCAAATCCAGGATGTCAATATGTATATCAGGACCAAACAACAATGCATCTGCAGTAGTATGGTACAACAGAAGGCCTGTTGCAGAAATTAACACATGGGCCCGAAACATACTAAGAACACAGGAATCTGAATGTGTATGCCACAACGGCGTATGCCCAGTAGTGTTCACCGATGGGTCTGCCACTGGACCTGCAGACACAAGAATATACTATTTTAAAGAGGGGAAAATATTGAAATGGGAGTCTCTGACTGGAACTGCTAAGCATATTGAAGAATGCTCATGTTACGGGGAACGAACAGGAATTACCTGCACATGCAGGGACAATTGGCAGGGCTCAAATAGACCAGTGATTCAGATAGACCCAGTAGCAATGACACACACTAGTCAATATATATGCAGTCCTGTTCTTACAGACAATCCCCGACCGAATGACCCAAATATAGGTAAGTGTAATGACCCTTATCCAGGTAATAATAACAATGGAGTCAAGGGATTCTCATACCTGGATGGGGCTAACACTTGGCTAGGGAGGACAATAAGCACAGCCTCGAGGTCTGGATACGAGATGTTAAAAGTGCCAAATGCATTGACAGATGATAGATCAAAGCCCATTCAAGGTCAGACAATTGTATTAAACGCTGACTGGAGTGGTTACAGTGGATCTTTCATGGACTATTGGGCTGAAGGGGACTGCTATCGAGCGTGTTTTTATGTGGAGTTGATACGTGGAAGACCCAAGGAGGATAAAGTGTGGTGGACCAGCAATAGTATAGTATCGATGTGTTCCAGTACAGAATTCCTGGGACAATGGAACTGGCCTGATGGGGCTAAAATAGAGTACTTCCTCTAA
ATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTCTCTATCATTCCATCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAGGATGTTTTTGCAGGGAAGAACGCAGATCTCGAGGCTCTCATGGAGTGGATAAAGACAAGACCAATCCTGTCACCTCTGACTAAGGGGATTTTAGGGTTTGTGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGGTTTGTCCAAAACGCCCTAAATGGGAATGGAGACCCAAACAACATGGACAAGGCGGTTAAATTATACAAGAAACTGAAGAGGGAAATGACATTTCATGGAGCAAAGGAAGTTGCACTCAGTTACTCAACTGGTGCGCTTGCCAGCTGCATGGGTCTCATATACAACAGAATGGGGACTGTGACCGCAGAAGGGGCTCTTGGACTAGTATGTGCCACTTGTGAGCAGATTGCTGACGCACAACATCGGTCCCACAGGCAGATGGCGACTACTACTAACCCACTAATTAGGCATGAGAATAGAATGGTACTAGCCAGCACTACGGCTAAGGCTATGGAGCAGATGGCTGGATCAAGTGAACAGGCAGCGGAAGCCATGGAAGTTGCAAGTCAGGCTAGGCAAATGGTGCAGGCTATGAGAACAGTTGGGACTCACCCTAACTCCAGTACAGGTCTAAAAGATGATCTTATTGAAAATTTGCAGGCCTACCAGAACCGGATGGGAGTGCAACTGCAGCGGTTCAAGTGAGCCTCTAGTCGTTGCAGCTAACATTATTGGGATATTGCACTTGATATTGTGGATTCTTGATCGTCTTTTCTTCAAATGCATTTATCGTCGTTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAAGGAATGCCTGAGTCTATGAGGGAAGAATATCGGCAGGAACAGCAGAATGCTGTGGATGTTGACGATGGTCATTTTGTCAACATAGAGCTGAAGTAA
ATGGATTCCAATACTGTGTCAAGCTTCCAGGTAGACTGCTTTCTTTGGCATGTCCGCAAACGATTTGCAGACCAAGAAATGGGTGATGCCCCATTTCTAGACCGGCTTCGCCGAGATCAGAAGTCCCTGAGAGGAAGAAGCAGCACTCTTGGTCTGGACATCAGAACTGCCACGCGTGAAGGAAAGCATATAGTGGAGCGGATTTTAGAGGAAGAGTCAGATGAAGCATTTAAAATGAGTATTGCTTCAGTGCCAGCTCCACGCTATCTAACTGACATGACTCTTGAAGAAATGTCAAGAGATTGGTTAATGCTCATTCCCAAACAGAAAATAACAGGGTCCCTATGCATTAGAATGGACCAAGCAATAGTGGACAAAAACATCACATTGAAAGCAAATTTCAGTGTGATTTTCAATCGGCTTGAAGCCCTGATACTACTTAGAGCTTTTACGGAAGAAGGAGCAATTGTAGGCGAAATCTCACCATTACCTTCTCTTCCAGGACATACTGACAAGGATGTCAAAAATGCAATTGAGATCCTCATCGGAGGATTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAAGCAGCGATGAGGATGGGAGATCTCCACTCTCTACAAAGTAGAAACGGGAAATGGAGAGAACAGTTAAGCCAGAAGTTCGAAGAAATAAGATGGTTGATTGAAGAAGTACGACATAGATTAAAAATTACGGAGAATAGCTTTGAGCAAATAACTTTTATGCAAGCCTTACAACTATTGCTTGAAGTGGAGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAA

[ 2013/04/20,04:44ET changed: A1879G(1),A744G(3),C1178T(3) ]
[ 2013/04/29,07:01ET changed: T704A(4) {235Q,poultry-like} ]
[ 2013/05/02,02:53ET changed it back: A704T(4) ]