I'm searching for the amino acid, nucleotide coding and what each one does and what segment it's found in. Any help with this would be much appreciated.
Phenotype Marker from GenBank (** Panflu common markers)
Segments:
PB2(1) PB1(2) PA(3) HA(4) NP(5) NA(6) MP(7) NS(8)
Adamatane Resistance
M2: 26F
M2: 27A
M2: 30T
M2: S31N **
M2: 34E
Oseltamivir Resistance
NA: H275Y (C823T)
Enhanced Transmission
M2: A16G
M2: C55F **
NP: L136M
PA: S409N **
PB2: A199S
PB2: A661T
PB2: K702R
PB2: V667I
Increased Virulence
PB2: D701N
PB2: E627K
Severity
NS1:T92E
Polybasic HA Cleavage (gs: only for H5 and H7)
RERRRKKR
Other H1N1 mutations we are watching:
PB2: K340N (A1020T)
HA: D225N (715A), D225G (716G), D225E (717A or G)
HA: M230 730, 730, 732 ATG; M230I = AT(t,c,a?)
When cross-linked and possibly associated with mutations at 225
HA: syn413K (A1281G)
NA: syn407V (T1221C)
Segments:
PB2(1) PB1(2) PA(3) HA(4) NP(5) NA(6) MP(7) NS(8)
What group for these?: A193T, S193F, (HA in H3N2 ?)
PB2: A199S, A661T, E627K, V667I, D701N, K702R,
PB1:
PA:
HA: D225N, D225G, I324V
NP:
NA: E119V, R152K, H274Y, R292K
MP:
NS:
Further explanation about resistance: the amino acid termed E276 must rotate and bond with R224 to form a pocket for the side chain of Oseltamivir. The mutations R292K, N294S, and H274Y inhibit this rotation and prevent the pocket from forming, resulting in resistance to Oseltamivir. An E119V mutation also interferes only with oseltamivir binding, possibly because a water molecule can fit between oseltamivir and valine at the active site but cannot insinuate itself between zanamivir and valine at residue 119.
Phenotype Marker from GenBank (** Panflu common markers)
Segments:
PB2(1) PB1(2) PA(3) HA(4) NP(5) NA(6) MP(7) NS(8)
Adamatane Resistance
M2: 26F
M2: 27A
M2: 30T
M2: S31N **
M2: 34E
Oseltamivir Resistance
NA: H275Y (C823T)
Enhanced Transmission
M2: A16G
M2: C55F **
NP: L136M
PA: S409N **
PB2: A199S
PB2: A661T
PB2: K702R
PB2: V667I
Increased Virulence
PB2: D701N
PB2: E627K
Severity
NS1:T92E
Polybasic HA Cleavage (gs: only for H5 and H7)
RERRRKKR
Other H1N1 mutations we are watching:
PB2: K340N (A1020T)
HA: D225N (715A), D225G (716G), D225E (717A or G)
HA: M230 730, 730, 732 ATG; M230I = AT(t,c,a?)
When cross-linked and possibly associated with mutations at 225
HA: syn413K (A1281G)
NA: syn407V (T1221C)
Segments:
PB2(1) PB1(2) PA(3) HA(4) NP(5) NA(6) MP(7) NS(8)
What group for these?: A193T, S193F, (HA in H3N2 ?)
PB2: A199S, A661T, E627K, V667I, D701N, K702R,
PB1:
PA:
HA: D225N, D225G, I324V
NP:
NA: E119V, R152K, H274Y, R292K
MP:
NS:
Further explanation about resistance: the amino acid termed E276 must rotate and bond with R224 to form a pocket for the side chain of Oseltamivir. The mutations R292K, N294S, and H274Y inhibit this rotation and prevent the pocket from forming, resulting in resistance to Oseltamivir. An E119V mutation also interferes only with oseltamivir binding, possibly because a water molecule can fit between oseltamivir and valine at the active site but cannot insinuate itself between zanamivir and valine at residue 119.
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