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J Clin Virol. Comparison of commercial influenza A virus assays in detecting avian influenza H7N9 among poultry cloacal swabs, China.
[Source: US National Library of Medicine, full page: (LINK). Abstract, edited.]
J Clin Virol. 2014 Jan 24. pii: S1386-6532(14)00029-8. doi: 10.1016/j.jcv.2014.01.009. [Epub ahead of print]
Comparison of commercial influenza A virus assays in detecting avian influenza H7N9 among poultry cloacal swabs, China.
Ma MJ<SUP>1</SUP>, Yang XX<SUP>1</SUP>, Xia X<SUP>1</SUP>, Anderson BD<SUP>2</SUP>, Heil GL<SUP>2</SUP>, Qian YH<SUP>3</SUP>, Lu B<SUP>3</SUP>, Cao WC<SUP>4</SUP>, Gray GC<SUP>5</SUP>.
Author information: <SUP>1</SUP>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China. <SUP>2</SUP>Department of Environmental & Global Health, College of Public Health & Health Professions, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA. <SUP>3</SUP>Department of Disease Prevention and Control, Wuxi Center for Disease Control and Prevention, Wuxi 214023, PR China. <SUP>4</SUP>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China. Electronic address: caowc@bmi.ac.cn. <SUP>5</SUP>Department of Environmental & Global Health, College of Public Health & Health Professions, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA. Electronic address: gcgray@phhp.ufl.edu.
Abstract
BACKGROUND:
Avian H7N9 virus emerged in China in February 2013 and has since spread widely among China's poultry, causing numerous human infections.
OBJECTIVES:
To compare World Health Organization (WHO) and US commercial influenza assays in detecting avian H7N9 virus in poultry cloacal specimens.
STUDY DESIGN:
Between April 6 and July 15, 2013, 261 cloacal swabs were collected from commercial poultry in Nanjing and Wuxi City, Jiangsu Province, China. Swabs were screened with the WHO's influenza A and H7N9 real-time RT-PCR (qRT-PCR) assays. A blinded panel of 97 specimens (27 H7N9-positive and 70 influenza A-negative) was then used to compare 3 antigen based commercial assays (Remel Xpect Flu A&B, Quidel Quickvue influenza, and Quidel Sofia Influenza A+B), and 2 molecular commercial assays (Quidel Molecular Influenza A+B assay and Life Technologies VetMAX?-Gold SIV Detection Kit). None of these commercial assays were approved for use with poultry specimens.
RESULTS:
Considering the WHO H7N9 qRT-PCR assay as the gold standard, all assays except the Quidel Quickvue influenza assay had high specificity (ranging from 96 to 99%). Regarding sensitivity, the Life Technologies VetMAX?-Gold SIV Detection Kit (100%; 95% CI 87-100%) and the Quidel Molecular Influenza A+B assay (85%; 95% CI 66-96%) performed the best. The sensitivities of the non-molecular antigen detection assays were either unable to detect small amounts of H7N9 viral RNA or were inhibited by specimen type.
CONCLUSIONS:
The Life Technologies VetMAX?-Gold SIV Detection Kit and the Quidel Molecular Influenza A+B assay are comparable in performance to the WHO H7N9 qRT-PCR assay in detecting H7N9 from poultry cloacal specimens.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS: Avian influenza A virus, Diagnostic assay, Epidemiology, Virology, Zoonoses
PMID: 24529843 [PubMed - as supplied by publisher]
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J Clin Virol. 2014 Jan 24. pii: S1386-6532(14)00029-8. doi: 10.1016/j.jcv.2014.01.009. [Epub ahead of print]
Comparison of commercial influenza A virus assays in detecting avian influenza H7N9 among poultry cloacal swabs, China.
Ma MJ<SUP>1</SUP>, Yang XX<SUP>1</SUP>, Xia X<SUP>1</SUP>, Anderson BD<SUP>2</SUP>, Heil GL<SUP>2</SUP>, Qian YH<SUP>3</SUP>, Lu B<SUP>3</SUP>, Cao WC<SUP>4</SUP>, Gray GC<SUP>5</SUP>.
Author information: <SUP>1</SUP>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China. <SUP>2</SUP>Department of Environmental & Global Health, College of Public Health & Health Professions, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA. <SUP>3</SUP>Department of Disease Prevention and Control, Wuxi Center for Disease Control and Prevention, Wuxi 214023, PR China. <SUP>4</SUP>State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, PR China. Electronic address: caowc@bmi.ac.cn. <SUP>5</SUP>Department of Environmental & Global Health, College of Public Health & Health Professions, and Emerging Pathogens Institute, University of Florida, Gainesville, FL 32610, USA. Electronic address: gcgray@phhp.ufl.edu.
Abstract
BACKGROUND:
Avian H7N9 virus emerged in China in February 2013 and has since spread widely among China's poultry, causing numerous human infections.
OBJECTIVES:
To compare World Health Organization (WHO) and US commercial influenza assays in detecting avian H7N9 virus in poultry cloacal specimens.
STUDY DESIGN:
Between April 6 and July 15, 2013, 261 cloacal swabs were collected from commercial poultry in Nanjing and Wuxi City, Jiangsu Province, China. Swabs were screened with the WHO's influenza A and H7N9 real-time RT-PCR (qRT-PCR) assays. A blinded panel of 97 specimens (27 H7N9-positive and 70 influenza A-negative) was then used to compare 3 antigen based commercial assays (Remel Xpect Flu A&B, Quidel Quickvue influenza, and Quidel Sofia Influenza A+B), and 2 molecular commercial assays (Quidel Molecular Influenza A+B assay and Life Technologies VetMAX?-Gold SIV Detection Kit). None of these commercial assays were approved for use with poultry specimens.
RESULTS:
Considering the WHO H7N9 qRT-PCR assay as the gold standard, all assays except the Quidel Quickvue influenza assay had high specificity (ranging from 96 to 99%). Regarding sensitivity, the Life Technologies VetMAX?-Gold SIV Detection Kit (100%; 95% CI 87-100%) and the Quidel Molecular Influenza A+B assay (85%; 95% CI 66-96%) performed the best. The sensitivities of the non-molecular antigen detection assays were either unable to detect small amounts of H7N9 viral RNA or were inhibited by specimen type.
CONCLUSIONS:
The Life Technologies VetMAX?-Gold SIV Detection Kit and the Quidel Molecular Influenza A+B assay are comparable in performance to the WHO H7N9 qRT-PCR assay in detecting H7N9 from poultry cloacal specimens.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS: Avian influenza A virus, Diagnostic assay, Epidemiology, Virology, Zoonoses
PMID: 24529843 [PubMed - as supplied by publisher]
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