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Emerg Infect Dis. Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
[Source: Emerging Infectious Diseases Journal, full page: (LINK). Abstract, edited.]
Volume 19, Number 10?October 2013
Dispatch
Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
Chao Qiu<SUP>1</SUP>, Yang Huang<SUP>1</SUP>, Anli Zhang<SUP>1</SUP>, Di Tian<SUP>1</SUP>, Yanmin Wan<SUP>1</SUP>, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, and Jianqing Xu
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China (C. Qiu, A. Zhang, D. Tian, Y. Wan, Xiaoling Zhang, W. Zhang, Z. Zhang, Z. Yuan, Y. Hu, Xiaoyan Zhang, J. Xu); Fudan University, Shanghai (C. Qiu, Y. Huang, A. Zhang, Z. Yuan, Xiaoyan Zhang, J. Xu); Chinese Center for Disease Control and Prevention, Beijing, China (Xiaoyan Zhang, J. Xu)
Abstract
Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus?based neutralization assay with preliminary assessment using subtype H7N9?infected samples and controls.
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Volume 19, Number 10?October 2013
Dispatch
Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus
Chao Qiu<SUP>1</SUP>, Yang Huang<SUP>1</SUP>, Anli Zhang<SUP>1</SUP>, Di Tian<SUP>1</SUP>, Yanmin Wan<SUP>1</SUP>, Xiaoling Zhang, Wanju Zhang, Zhiyong Zhang, Zhenghong Yuan, Yunwen Hu, Xiaoyan Zhang, and Jianqing Xu
Author affiliations: Shanghai Public Health Clinical Center, Shanghai, China (C. Qiu, A. Zhang, D. Tian, Y. Wan, Xiaoling Zhang, W. Zhang, Z. Zhang, Z. Yuan, Y. Hu, Xiaoyan Zhang, J. Xu); Fudan University, Shanghai (C. Qiu, Y. Huang, A. Zhang, Z. Yuan, Xiaoyan Zhang, J. Xu); Chinese Center for Disease Control and Prevention, Beijing, China (Xiaoyan Zhang, J. Xu)
Abstract
Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus?based neutralization assay with preliminary assessment using subtype H7N9?infected samples and controls.
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