tetano
Editor, Senior Moderator
Viruses
. 2020 Dec 30;13(1):E46.
doi: 10.3390/v13010046.
PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
Sijia He[SUP] 1 [/SUP], Abdul A Waheed[SUP] 2 [/SUP], Brian Hetrick[SUP] 1 [/SUP], Deemah Dabbagh[SUP] 1 [/SUP], Ivan V Akhrymuk[SUP] 3 [/SUP], Kylene Kehn-Hall[SUP] 3 [/SUP], Eric O Freed[SUP] 2 [/SUP], Yuntao Wu[SUP] 1 [/SUP]
Affiliations
Abstract
P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1[SUP]+[/SUP] cells.
Keywords: cellular antiviral factor; coronavirus; pseudovirion.
. 2020 Dec 30;13(1):E46.
doi: 10.3390/v13010046.
PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
Sijia He[SUP] 1 [/SUP], Abdul A Waheed[SUP] 2 [/SUP], Brian Hetrick[SUP] 1 [/SUP], Deemah Dabbagh[SUP] 1 [/SUP], Ivan V Akhrymuk[SUP] 3 [/SUP], Kylene Kehn-Hall[SUP] 3 [/SUP], Eric O Freed[SUP] 2 [/SUP], Yuntao Wu[SUP] 1 [/SUP]
Affiliations
- PMID: 33396594
- DOI: 10.3390/v13010046
Abstract
P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1[SUP]+[/SUP] cells.
Keywords: cellular antiviral factor; coronavirus; pseudovirion.