tetano
Editor, Senior Moderator
Viruses
. 2020 Jun 8;12(6):E624.
doi: 10.3390/v12060624.
Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples
Boris Pastorino[SUP] 1 [/SUP], Franck Touret[SUP] 1 [/SUP], Magali Gilles[SUP] 1 [/SUP], Lea Luciani[SUP] 1 [/SUP], Xavier de Lamballerie[SUP] 1 [/SUP], Remi N Charrel[SUP] 1 [/SUP]
Affiliations
Abstract
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
Keywords: COVID-19; SARS-CoV-2; coronavirus; extraction buffer; inactivation; lysis buffer.
. 2020 Jun 8;12(6):E624.
doi: 10.3390/v12060624.
Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples
Boris Pastorino[SUP] 1 [/SUP], Franck Touret[SUP] 1 [/SUP], Magali Gilles[SUP] 1 [/SUP], Lea Luciani[SUP] 1 [/SUP], Xavier de Lamballerie[SUP] 1 [/SUP], Remi N Charrel[SUP] 1 [/SUP]
Affiliations
- PMID: 32521706
- DOI: 10.3390/v12060624
Abstract
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 minute contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.
Keywords: COVID-19; SARS-CoV-2; coronavirus; extraction buffer; inactivation; lysis buffer.