tetano
Editor, Senior Moderator
Virology
. 2022 Dec 31;579:38-45.
doi: 10.1016/j.virol.2022.12.013. Online ahead of print.
siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences
Riccardo Villa[SUP] 1 [/SUP], Sabrina Renzi[SUP] 1 [/SUP], Silvia Dotti[SUP] 1 [/SUP], Franco Lucchini[SUP] 2 [/SUP]
Affiliations
Abstract
Due to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, artificial siRNA pools targeting NP and PB genomic regions of the Influenza virus were produced in engineered Escherichia coli, adapting a published protocol. In a MDCK cell in vitro model, these preparations were challenged against reporter vectors bearing viral genomic sequences. A strong and specific RNA interference activity was observed, and the details of this action were indagated.
Keywords: Antiviral; Bacterial siRNA pools; Influenza virus; RNA interference; siRNA.
. 2022 Dec 31;579:38-45.
doi: 10.1016/j.virol.2022.12.013. Online ahead of print.
siRNAs pools generated in Escherichia coli exhibit strong RNA-interference activity against influenza virus genomic sequences
Riccardo Villa[SUP] 1 [/SUP], Sabrina Renzi[SUP] 1 [/SUP], Silvia Dotti[SUP] 1 [/SUP], Franco Lucchini[SUP] 2 [/SUP]
Affiliations
- PMID: 36599198
- DOI: 10.1016/j.virol.2022.12.013
Abstract
Due to the recurrent pandemic outbreaks that occurred during the last century, Influenza A viruses are considered a serious potential danger to human health. Among the innate immune pathways in eukaryotes, RNA interference plays a significant role in the interaction between viruses and host cells. RNA interference is addressed by small dsRNA molecules produced by the host itself (miRNAs, i.e. "micro-RNAs") but can be triggered also by the administration of exogenous short RNAs (siRNAs, "short interfering RNAs"). In this work, artificial siRNA pools targeting NP and PB genomic regions of the Influenza virus were produced in engineered Escherichia coli, adapting a published protocol. In a MDCK cell in vitro model, these preparations were challenged against reporter vectors bearing viral genomic sequences. A strong and specific RNA interference activity was observed, and the details of this action were indagated.
Keywords: Antiviral; Bacterial siRNA pools; Influenza virus; RNA interference; siRNA.