Virology. Productive replication of Middle East respiratory syndrome coronavirus in monocyte-derived dendritic cells modulates innate immune response

Giuseppe · Mar 7, 2014

[Source: ScienceDirect, full page: (LINK). Abstract, edited.]

Virology, Volumes 454?455, April 2014, Pages 197?205 / Brief Communication

Productive replication of Middle East respiratory syndrome coronavirus in monocyte-derived dendritic cells modulates innate immune response

Hin Chu a, 1, Jie Zhou a, b, c, 1, Bosco Ho-Yin Wong a, Cun Li a, Zhong-Shan Cheng a, Xiang Lin e, Vincent Kwok-Man Poon a, Tianhao Sun a, Candy Choi-Yi Lau a, Jasper Fuk-Woo Chan a, b, c, d, Kelvin Kai-Wang To a, b, c, d, Kwok-**** Chan a, Liwei Lu e, Bo-Jian Zheng a, b, c, d, Kwok-Yung Yuen a, b, c, d

a Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China - b State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China - c Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China - d Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China - e Department of Pathology, The University of Hong Kong, Hong Kong Special Administrative Region, China

Received 6 February 2014, Revised 6 February 2014, Accepted 14 February 2014, Available online 7 March 2014

http://dx.doi.org/10.1016/j.virol.2014.02.018

Highlights

MERS-CoV productively infected Mo-DCs while SARS-CoV-infection was abortive.
MERS-CoV induced higher levels of IFN-γ, IP-10, IL-12, and RANTES than SARS-CoV.
MERS-CoV induced higher surface expression of MHC II and CD86 than SARS-CoV.

Abstract

The Middle East respiratory syndrome coronavirus (MERS-CoV) closely resembled severe acute respiratory syndrome coronavirus (SARS-CoV) in disease manifestation as rapidly progressive acute pneumonia with multi-organ dysfunction. Using monocyte-derived-dendritic cells (Mo-DCs), we discovered fundamental discrepancies in the outcome of MERS‐CoV‐ and SARS-CoV-infection. First, MERS-CoV productively infected Mo-DCs while SARS-CoV-infection was abortive. Second, MERS-CoV induced significantly higher levels of IFN-γ, IP-10, IL-12, and RANTES expression than SARS-CoV. Third, MERS-CoV-infection induced higher surface expression of MHC class II (HLA-DR) and the co-stimulatory molecule CD86 than SARS-CoV-infection. Overall, our data suggests that the dendritic cell can serve as an important target of viral replication and a vehicle for dissemination. MERS-CoV-infection in DCs results in the production of a rich combination of cytokines and chemokines, and modulates innate immune response differently from that of SARS-CoV-infection. Our findings may help to explain the apparent discrepancy in the pathogenicity between MERS-CoV and SARS-CoV.

Keywords

MERS-CoV; SARS-CoV; Viral replication; Pathogenesis; Cytokine and chemokine response; Antigen-presentation

Corresponding author at: Carol Yu Centre for Infection, Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Pokfulam, Hong Kong Special Administrative Region, China. Tel.: +852 225 548 97; fax: +852 2855 1241.

1 These authors contributed equally to the study.

Copyright ? 2014 Elsevier Inc. All rights reserved.

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Virology. Productive replication of Middle East respiratory syndrome coronavirus in monocyte-derived dendritic cells modulates innate immune response

Giuseppe

Emeritus