Giuseppe
Emeritus
Modeling host responses in ferrets during A/California/07/2009 influenza infection. (Virology, abstract, edited)
[Source: US National Library of Medicine, (LINK). Edited.]
Virology. 2010 Mar 22. [Epub ahead of print]
Modeling host responses in ferrets during A/California/07/2009 influenza infection.
Rowe T, Le?n AJ, Crevar CJ, Carter DM, Xu L, Ran L, Fang Y, Cameron CM, Cameron MJ, Banner D, Ng DC, Ran R, Weirback HK, Wiley CA, Kelvin DJ, Ross TM. - International Institute of Infection and Immunity at Shantou Medical College, 22 Xingling Rd, Shantou, Guangdong 515041, People's Republic of China; University Health Network, 101 College St, Toronto, Ontario, Canada M5G1L7; Institute of Medical Sciences, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S1A8.
Immune responses during infection with pandemic H1N1 2009 influenza A virus (2009-H1N1) are still poorly understood. Using an experimental infection model in ferrets, we examined the pathological features and characterized the host immune responses by using microarray analysis, during infection with 2009-H1N1 A/California/07/2009 and seasonal A/Brisbane/59/2007. Chemokines CCL2, CCL8, CXCL7 and CXCL10 along with the majority of interferon-stimulated genes were expressed early, correlated to lung pathology, and abruptly decreased expression on day 7 following infection of A/California/07/2009. Interestingly, the drop in innate immune gene expression was replaced by a significant increase of the adaptive immune genes for granzymes and immunoglobulins. Serum anti-influenza antibodies were first observed on day 7, commensurate with the viral clearance. We propose that lung pathology in humans occurs during the innate phase of host immunity and a delay or failure to switch to the adaptive phase may contribute to morbidity and mortality during severe 2009-H1N1 infections.
Copyright ? 2010 Elsevier Inc. All rights reserved.
PMID: 20334888 [PubMed - as supplied by publisher]
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[Source: US National Library of Medicine, (LINK). Edited.]
Virology. 2010 Mar 22. [Epub ahead of print]
Modeling host responses in ferrets during A/California/07/2009 influenza infection.
Rowe T, Le?n AJ, Crevar CJ, Carter DM, Xu L, Ran L, Fang Y, Cameron CM, Cameron MJ, Banner D, Ng DC, Ran R, Weirback HK, Wiley CA, Kelvin DJ, Ross TM. - International Institute of Infection and Immunity at Shantou Medical College, 22 Xingling Rd, Shantou, Guangdong 515041, People's Republic of China; University Health Network, 101 College St, Toronto, Ontario, Canada M5G1L7; Institute of Medical Sciences, University of Toronto, 1 King's College Circle, Toronto, ON, Canada M5S1A8.
Immune responses during infection with pandemic H1N1 2009 influenza A virus (2009-H1N1) are still poorly understood. Using an experimental infection model in ferrets, we examined the pathological features and characterized the host immune responses by using microarray analysis, during infection with 2009-H1N1 A/California/07/2009 and seasonal A/Brisbane/59/2007. Chemokines CCL2, CCL8, CXCL7 and CXCL10 along with the majority of interferon-stimulated genes were expressed early, correlated to lung pathology, and abruptly decreased expression on day 7 following infection of A/California/07/2009. Interestingly, the drop in innate immune gene expression was replaced by a significant increase of the adaptive immune genes for granzymes and immunoglobulins. Serum anti-influenza antibodies were first observed on day 7, commensurate with the viral clearance. We propose that lung pathology in humans occurs during the innate phase of host immunity and a delay or failure to switch to the adaptive phase may contribute to morbidity and mortality during severe 2009-H1N1 infections.
Copyright ? 2010 Elsevier Inc. All rights reserved.
PMID: 20334888 [PubMed - as supplied by publisher]
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