tetano
Editor, Senior Moderator
Virology
. 2021 Sep 11;563:134-145.
doi: 10.1016/j.virol.2021.09.001. Online ahead of print.
Contribution of single mutations to selected SARS-CoV-2 emerging variants spike antigenicity
Shang Yu Gong[SUP] 1 [/SUP], Debashree Chatterjee[SUP] 2 [/SUP], Jonathan Richard[SUP] 3 [/SUP], Jérémie Prévost[SUP] 3 [/SUP], Alexandra Tauzin[SUP] 3 [/SUP], Romain Gasser[SUP] 3 [/SUP], Yuxia Bo[SUP] 4 [/SUP], Dani Vézina[SUP] 3 [/SUP], Guillaume Goyette[SUP] 2 [/SUP], Gabrielle Gendron-Lepage[SUP] 2 [/SUP], Halima Medjahed[SUP] 2 [/SUP], Michel Roger[SUP] 5 [/SUP], Marceline Côté[SUP] 4 [/SUP], Andrés Finzi[SUP] 6 [/SUP]
Affiliations
Abstract
Towards the end of 2020, multiple variants of concern (VOCs) and variants of interest (VOIs) have arisen from the original SARS-CoV-2 Wuhan-Hu-1 strain. Mutations in the Spike protein are highly scrutinized for their impact on transmissibility, pathogenesis and vaccine efficacy. Here, we contribute to the growing body of literature on emerging variants by evaluating the impact of single mutations on the overall antigenicity of selected variants and their binding to the ACE2 receptor. We observe a differential contribution of single mutants to the global variants phenotype related to ACE2 interaction and antigenicity. Using biolayer interferometry, we observe that enhanced ACE2 interaction is mostly modulated by a decrease in off-rate. Finally, we made the interesting observation that the Spikes from tested emerging variants bind better to ACE2 at 37°C compared to the D614G variant. Whether improved ACE2 binding at higher temperature facilitates emerging variants transmission remain to be demonstrated.
Keywords: ACE2; COVID-19; Coronavirus; RBD; SARS-CoV-2; Spike glycoproteins; Temperature; Vaccines; Variants of concern.
. 2021 Sep 11;563:134-145.
doi: 10.1016/j.virol.2021.09.001. Online ahead of print.
Contribution of single mutations to selected SARS-CoV-2 emerging variants spike antigenicity
Shang Yu Gong[SUP] 1 [/SUP], Debashree Chatterjee[SUP] 2 [/SUP], Jonathan Richard[SUP] 3 [/SUP], Jérémie Prévost[SUP] 3 [/SUP], Alexandra Tauzin[SUP] 3 [/SUP], Romain Gasser[SUP] 3 [/SUP], Yuxia Bo[SUP] 4 [/SUP], Dani Vézina[SUP] 3 [/SUP], Guillaume Goyette[SUP] 2 [/SUP], Gabrielle Gendron-Lepage[SUP] 2 [/SUP], Halima Medjahed[SUP] 2 [/SUP], Michel Roger[SUP] 5 [/SUP], Marceline Côté[SUP] 4 [/SUP], Andrés Finzi[SUP] 6 [/SUP]
Affiliations
- PMID: 34536797
- PMCID: PMC8433594
- DOI: 10.1016/j.virol.2021.09.001
Abstract
Towards the end of 2020, multiple variants of concern (VOCs) and variants of interest (VOIs) have arisen from the original SARS-CoV-2 Wuhan-Hu-1 strain. Mutations in the Spike protein are highly scrutinized for their impact on transmissibility, pathogenesis and vaccine efficacy. Here, we contribute to the growing body of literature on emerging variants by evaluating the impact of single mutations on the overall antigenicity of selected variants and their binding to the ACE2 receptor. We observe a differential contribution of single mutants to the global variants phenotype related to ACE2 interaction and antigenicity. Using biolayer interferometry, we observe that enhanced ACE2 interaction is mostly modulated by a decrease in off-rate. Finally, we made the interesting observation that the Spikes from tested emerging variants bind better to ACE2 at 37°C compared to the D614G variant. Whether improved ACE2 binding at higher temperature facilitates emerging variants transmission remain to be demonstrated.
Keywords: ACE2; COVID-19; Coronavirus; RBD; SARS-CoV-2; Spike glycoproteins; Temperature; Vaccines; Variants of concern.