tetano
Editor, Senior Moderator
Virology
. 2026 May 2:621:110938.
doi: 10.1016/j.virol.2026.110938. Online ahead of print.
CD25[SUP]+[/SUP] cell depletion enhances protective immunity of PR8 inactivated influenza vaccine in aged mice
Ying Zhang[SUP] 1 [/SUP], Lingling Liu[SUP] 1 [/SUP], Tengfei Chen[SUP] 1 [/SUP], Mingqi Li[SUP] 1 [/SUP], Xiaodan Shi[SUP] 1 [/SUP], Ying Liu[SUP] 2 [/SUP]
Affiliations
Immunosenescence is a real biological process that leads to a quantitatively and qualitatively weaker immune response to vaccines in the aged population. The adaptive immune system, especially B cells and T cells, is responsible for creating targeted, long-lasting immune memory and is the component most directly responsible for vaccine efficacy. However, this system undergoes substantial functional decline with aging. This study evaluated whether depletion CD25[SUP]+[/SUP] cell in combination with a saponin-based ISCOMs adjuvant enhances the efficacy of PR8 inactivated influenza vaccine in aged mice. Aged BALB/c mice were depleted of CD25[SUP]+[/SUP] cells using an anti-CD25 monoclonal antibody prior to intramuscular vaccination with inactivated PR8 virus, either with or without the ISCOMs adjuvant. The combination of CD25[SUP]+[/SUP] depletion and the adjuvant induced robust antibody responses, promoted a balanced IgG2a/IgG1 ratio, significantly elevated hemagglutination inhibition titers, markedly reduced lung viral loads, and provided complete protection against a lethal challenge. These results demonstrate that targeting CD25 signaling in combination with a saponin-based adjuvant offers a promising strategy to overcome immunosenescence and enhance influenza vaccine efficacy in the elderly.
Keywords: Aged mice; CD25; ISCOMs; Influenza vaccine.
. 2026 May 2:621:110938.
doi: 10.1016/j.virol.2026.110938. Online ahead of print.
CD25[SUP]+[/SUP] cell depletion enhances protective immunity of PR8 inactivated influenza vaccine in aged mice
Ying Zhang[SUP] 1 [/SUP], Lingling Liu[SUP] 1 [/SUP], Tengfei Chen[SUP] 1 [/SUP], Mingqi Li[SUP] 1 [/SUP], Xiaodan Shi[SUP] 1 [/SUP], Ying Liu[SUP] 2 [/SUP]
Affiliations
- PMID: 42107283
- DOI: 10.1016/j.virol.2026.110938
Immunosenescence is a real biological process that leads to a quantitatively and qualitatively weaker immune response to vaccines in the aged population. The adaptive immune system, especially B cells and T cells, is responsible for creating targeted, long-lasting immune memory and is the component most directly responsible for vaccine efficacy. However, this system undergoes substantial functional decline with aging. This study evaluated whether depletion CD25[SUP]+[/SUP] cell in combination with a saponin-based ISCOMs adjuvant enhances the efficacy of PR8 inactivated influenza vaccine in aged mice. Aged BALB/c mice were depleted of CD25[SUP]+[/SUP] cells using an anti-CD25 monoclonal antibody prior to intramuscular vaccination with inactivated PR8 virus, either with or without the ISCOMs adjuvant. The combination of CD25[SUP]+[/SUP] depletion and the adjuvant induced robust antibody responses, promoted a balanced IgG2a/IgG1 ratio, significantly elevated hemagglutination inhibition titers, markedly reduced lung viral loads, and provided complete protection against a lethal challenge. These results demonstrate that targeting CD25 signaling in combination with a saponin-based adjuvant offers a promising strategy to overcome immunosenescence and enhance influenza vaccine efficacy in the elderly.
Keywords: Aged mice; CD25; ISCOMs; Influenza vaccine.