tetano
Editor, Senior Moderator
Virology
. 2025 Mar 19:606:110510.
doi: 10.1016/j.virol.2025.110510. Online ahead of print. A strategy of enhancing the protective efficacy of seasonal influenza vaccines by providing additional immunity to neuraminidase and M2e
Jannatul Ruhan Raha[SUP] 1 [/SUP], Ki-Hye Kim[SUP] 1 [/SUP], Chau Thuy Tien Le[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Rong Liu[SUP] 1 [/SUP], Phillip Grovenstein[SUP] 1 [/SUP], Surya Sekhar Pal[SUP] 1 [/SUP], Mahmuda Yeasmin[SUP] 1 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 2 [/SUP]
Affiliations
It is a high priority to enhance the efficacy of seasonal influenza vaccines based on hemagglutinin (HA) strain-specific neutralizing immunity. Here, we investigated a vaccination strategy of supplementing inactivated split seasonal vaccines with a virus-like particle vaccine containing multi-subtype neuraminidase (NA) and M2 ectodomain (M2e) repeat (NA-M2e) in mice. NA-M2e and split combined vaccine (S + NA-M2e) stimulated a unique pattern of innate immune responses within a day after intramuscular injection of mice. The combined S + NA-M2e vaccination induced enhanced levels of IgG antibodies to viral antigens, hemagglutination inhibiting activities, and humoral and cellular immune responses to NA and M2e. The addition of NA-M2e to split vaccination provided higher efficacy of protection against homologous and heterologous viruses compared to split alone, where NA-M2e significantly contributed to enhancing protection under naïve and primed mouse models. This study supports a vaccination strategy to improve the efficacy of seasonal vaccines by providing additional immunity to NA and M2e.
Keywords: Adaptive immunity; Cross-protection; Innate immune responses; NA-M2e universal vaccine; Seasonal vaccine; Supplemented vaccination.
. 2025 Mar 19:606:110510.
doi: 10.1016/j.virol.2025.110510. Online ahead of print. A strategy of enhancing the protective efficacy of seasonal influenza vaccines by providing additional immunity to neuraminidase and M2e
Jannatul Ruhan Raha[SUP] 1 [/SUP], Ki-Hye Kim[SUP] 1 [/SUP], Chau Thuy Tien Le[SUP] 1 [/SUP], Noopur Bhatnagar[SUP] 1 [/SUP], Rong Liu[SUP] 1 [/SUP], Phillip Grovenstein[SUP] 1 [/SUP], Surya Sekhar Pal[SUP] 1 [/SUP], Mahmuda Yeasmin[SUP] 1 [/SUP], Chong Hyun Shin[SUP] 1 [/SUP], Bao-Zhong Wang[SUP] 1 [/SUP], Sang-Moo Kang[SUP] 2 [/SUP]
Affiliations
- PMID: 40139072
- DOI: 10.1016/j.virol.2025.110510
It is a high priority to enhance the efficacy of seasonal influenza vaccines based on hemagglutinin (HA) strain-specific neutralizing immunity. Here, we investigated a vaccination strategy of supplementing inactivated split seasonal vaccines with a virus-like particle vaccine containing multi-subtype neuraminidase (NA) and M2 ectodomain (M2e) repeat (NA-M2e) in mice. NA-M2e and split combined vaccine (S + NA-M2e) stimulated a unique pattern of innate immune responses within a day after intramuscular injection of mice. The combined S + NA-M2e vaccination induced enhanced levels of IgG antibodies to viral antigens, hemagglutination inhibiting activities, and humoral and cellular immune responses to NA and M2e. The addition of NA-M2e to split vaccination provided higher efficacy of protection against homologous and heterologous viruses compared to split alone, where NA-M2e significantly contributed to enhancing protection under naïve and primed mouse models. This study supports a vaccination strategy to improve the efficacy of seasonal vaccines by providing additional immunity to NA and M2e.
Keywords: Adaptive immunity; Cross-protection; Innate immune responses; NA-M2e universal vaccine; Seasonal vaccine; Supplemented vaccination.