tetano
Editor, Senior Moderator
Virol J
. 2026 Jan 30.
doi: 10.1186/s12985-025-03006-z. Online ahead of print.
The nanoemulsion adjuvant provides antigen dose-sparing effects and enhances maternal passive immune protection for the cell-cultured quadrivalent influenza virus subunit vaccine
Yutian Wang[SUP] #[/SUP][SUP] 1 2 [/SUP], Zheng Jia[SUP] #[/SUP][SUP] 1 2 [/SUP], Ying Liu[SUP] 1 2 [/SUP], Shuang Li[SUP] 1 2 [/SUP], Yongbo Qiao[SUP] 1 2 [/SUP], Dapeng Zhao[SUP] 1 2 [/SUP], Jianyang Gu[SUP] #[/SUP][SUP] 3 4 [/SUP], Yehong Wu[SUP] #[/SUP][SUP] 5 6 [/SUP]
Affiliations
To elicit a robust immune response, an adjuvant can be combined with the antigen in influenza vaccine formulations. In this study, we evaluated the dose-sparing effect and safety of a squalene-based oil-in-water nanoemulsion (NE) adjuvant formulated with a cell culture-derived quadrivalent influenza vaccine. Immune responses-including anti-HA IgG antibody levels and hemagglutination inhibition (HAI) titers-were assessed, along with protection against a homologous challenge with influenza B virus (strain B/Maryland/15/2016 B/Victoria). We also investigated the influence of antigen dose on vaccine-induced immunity and the passive protection conferred to offspring via maternal antibody transfer. The NE adjuvant elicited strong anti-HA antibody responses in young adult mice, and these antibodies were effectively transferred from immunized mothers to their offspring. Furthermore, offspring born to NE-immunized mothers were protected against influenza virus challenge. Collectively, our results indicate that the NE formulation induces potent influenza-specific immune responses with dose-sparing effects and enables maternal transfer of protective immunity. These findings support the potential of NE as an effective adjuvant for MDCK cell-based influenza subunit vaccines. Importance. This study demonstrates that a squalene-based nanoemulsion (NE) adjuvant significantly enhances the immunogenicity and dose-sparing capacity of cell culture-derived quadrivalent influenza vaccines. Key advances include: (1) NE-adjuvanted vaccines achieved a remarkable 125-fold antigen dose reduction while maintaining antibody titers comparable to high-dose formulations, addressing critical challenges in pandemic preparedness; (2) NE induced robust humoral and cellular immunity, including elevated anti-HA IgG (10-fold increase vs. non-adjuvanted vaccine), improved HI titers, and enhanced IFN-γ/CD8 + T-cell responses; (3) Unique maternal-offspring protection was demonstrated, with transferred maternal antibodies conferring 100% survival in offspring against viral challenge. These findings position NE as an effective adjuvant technology that simultaneously optimizes antigen use and broadens immune protection across age groups.
Keywords: Challenge protection; Dose-sparing; Immune effect; Nano emulsion adjuvant; Quadrivalent influenza vaccine; Subunit vaccine.
. 2026 Jan 30.
doi: 10.1186/s12985-025-03006-z. Online ahead of print.
The nanoemulsion adjuvant provides antigen dose-sparing effects and enhances maternal passive immune protection for the cell-cultured quadrivalent influenza virus subunit vaccine
Yutian Wang[SUP] #[/SUP][SUP] 1 2 [/SUP], Zheng Jia[SUP] #[/SUP][SUP] 1 2 [/SUP], Ying Liu[SUP] 1 2 [/SUP], Shuang Li[SUP] 1 2 [/SUP], Yongbo Qiao[SUP] 1 2 [/SUP], Dapeng Zhao[SUP] 1 2 [/SUP], Jianyang Gu[SUP] #[/SUP][SUP] 3 4 [/SUP], Yehong Wu[SUP] #[/SUP][SUP] 5 6 [/SUP]
Affiliations
- PMID: 41618423
- DOI: 10.1186/s12985-025-03006-z
To elicit a robust immune response, an adjuvant can be combined with the antigen in influenza vaccine formulations. In this study, we evaluated the dose-sparing effect and safety of a squalene-based oil-in-water nanoemulsion (NE) adjuvant formulated with a cell culture-derived quadrivalent influenza vaccine. Immune responses-including anti-HA IgG antibody levels and hemagglutination inhibition (HAI) titers-were assessed, along with protection against a homologous challenge with influenza B virus (strain B/Maryland/15/2016 B/Victoria). We also investigated the influence of antigen dose on vaccine-induced immunity and the passive protection conferred to offspring via maternal antibody transfer. The NE adjuvant elicited strong anti-HA antibody responses in young adult mice, and these antibodies were effectively transferred from immunized mothers to their offspring. Furthermore, offspring born to NE-immunized mothers were protected against influenza virus challenge. Collectively, our results indicate that the NE formulation induces potent influenza-specific immune responses with dose-sparing effects and enables maternal transfer of protective immunity. These findings support the potential of NE as an effective adjuvant for MDCK cell-based influenza subunit vaccines. Importance. This study demonstrates that a squalene-based nanoemulsion (NE) adjuvant significantly enhances the immunogenicity and dose-sparing capacity of cell culture-derived quadrivalent influenza vaccines. Key advances include: (1) NE-adjuvanted vaccines achieved a remarkable 125-fold antigen dose reduction while maintaining antibody titers comparable to high-dose formulations, addressing critical challenges in pandemic preparedness; (2) NE induced robust humoral and cellular immunity, including elevated anti-HA IgG (10-fold increase vs. non-adjuvanted vaccine), improved HI titers, and enhanced IFN-γ/CD8 + T-cell responses; (3) Unique maternal-offspring protection was demonstrated, with transferred maternal antibodies conferring 100% survival in offspring against viral challenge. These findings position NE as an effective adjuvant technology that simultaneously optimizes antigen use and broadens immune protection across age groups.
Keywords: Challenge protection; Dose-sparing; Immune effect; Nano emulsion adjuvant; Quadrivalent influenza vaccine; Subunit vaccine.