tetano
Editor, Senior Moderator
Virol J
. 2024 Jul 11;21(1):156.
doi: 10.1186/s12985-024-02430-x. Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens
Li Xue[SUP] 1 [/SUP], Jianhong Zhu[SUP] 1 [/SUP], Ke Lei[SUP] 1 [/SUP], Zeshi Liu[SUP] 1 [/SUP], Yiwei Tang[SUP] 2 [/SUP], Bo Zhong[SUP] 3 [/SUP], Ning Gao[SUP] 1 [/SUP], Chaoliang Xiong[SUP] 1 [/SUP], Jing Lei[SUP] 1 [/SUP], Ying Tian[SUP] 1 [/SUP], Weixiao Zhou[SUP] 1 [/SUP], Nan Feng[SUP] 1 [/SUP], Xue Zhang[SUP] 1 [/SUP], Dong Chen[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Yan Geng[SUP] 4 [/SUP]
Affiliations
Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.
Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.
Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.
Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.
Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.
. 2024 Jul 11;21(1):156.
doi: 10.1186/s12985-024-02430-x. Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens
Li Xue[SUP] 1 [/SUP], Jianhong Zhu[SUP] 1 [/SUP], Ke Lei[SUP] 1 [/SUP], Zeshi Liu[SUP] 1 [/SUP], Yiwei Tang[SUP] 2 [/SUP], Bo Zhong[SUP] 3 [/SUP], Ning Gao[SUP] 1 [/SUP], Chaoliang Xiong[SUP] 1 [/SUP], Jing Lei[SUP] 1 [/SUP], Ying Tian[SUP] 1 [/SUP], Weixiao Zhou[SUP] 1 [/SUP], Nan Feng[SUP] 1 [/SUP], Xue Zhang[SUP] 1 [/SUP], Dong Chen[SUP] 1 [/SUP], Jing Li[SUP] 1 [/SUP], Yan Geng[SUP] 4 [/SUP]
Affiliations
- PMID: 38992721
- DOI: 10.1186/s12985-024-02430-x
Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.
Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.
Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.
Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.
Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.