Viral infection study - Infection of three rhesus macaques with French Polynesian Zika virus (Real-time data)

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Scientists Report In Real Time On Challenging Zika Research


NELL GREENFIELDBOYCE
Updated March 8, 20165:46 PM ETPublished March 8, 20165:00 PM ET

Anyone can follow the pregnancy of a monkey infected with Zika virus in real time, thanks to an experiment in data sharing that's unusual for biology.
Researchers in Wisconsin injected Zika virus into a pregnant rhesus macaque monkey on Monday, to start exploring how this virus can affect the brain of a developing fetus. Over the course of the coming weeks, the team will be posting the infected monkey's ultrasounds and blood tests, as well as other data such as the amount of virus in the amniotic fluid.

The study is being led by Dave O'Connor, a professor at the University of Wisconsin-Madison who is also writing a running commentary on the laboratory's website that frankly discusses things like the emotional impact of looking at this primate's ultrasounds.

O'Connor says that he can feel a moral need to do this kind of animal research and at the same time feel "sad and heartbroken" at what the work entails. "I don't think those two are mutually exclusive," he says.
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http://www.npr.org/sections/health-s...-zika-research

 

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Results[/h] [h=3]REAL-TIME EDITORIAL STUDY COMMENTARY[/h] [h=3]VIRAL CHALLENGE STOCK ANALYSIS[/h]

• Challenge stock sequence summary
• Raw Illumina reads R1 - FASTQ.gz | R2 - FASTQ.gz
• Mapping of reads from challenge stock to KJ776791 BAM mapping | BAI index
• Polymorphic sites in challenge stock relative to KJ776791 VCF
• KJ77691 sequence for mapping and variant calling (does not contain UTR extensions) FASTA
• Annotated challenge virus sequence (with additional UTR data not present in KJ776791) Geneious Pro | Genbank

[h=3]VIRAL RNA QUANTIFICATION[/h]

• Plasma viral loads Chart
• Pan urine viral loads Chart
• Cystocentisis viral loads Chart
• CSF viral loads Chart
• Oral swab viral loads Chart

[h=3]COMPLETE BLOOD COUNTS CHARTS[/h] [h=3]IMMUNOLOGY[/h]

• Natural killer cells Counts | Activated NK Chart
• Plasmablasts Wiki | Absolute counts in Excel
• T Cells Counts | Absolute Ki-67+ T Cells
• Luminex Plasma Raw Data

[h=3]BLOOD CHEMISTRY CHARTS[/h] [h=3]ANIMAL WEIGHTS CHARTS[/h] ...

https://zika.labkey.com/project/OConnor/ZIKV-001/begin.view