tetano
Editor, Senior Moderator
Vet Sci
. 2022 Jul 4;9(7):338.
doi: 10.3390/vetsci9070338.
A New Sampling Approach for the Detection of Swine Influenza a Virus on European Sow Farms
Kathrin Lillie-Jaschniski[SUP] 1 [/SUP], Marina Lisgara[SUP] 2 [/SUP], Emanuela Pileri[SUP] 3 [/SUP], Agnes Jardin[SUP] 4 [/SUP], Eduardo Velazquez[SUP] 5 [/SUP], Monika Köchling[SUP] 1 [/SUP], Michael Albin[SUP] 6 [/SUP], Carlos Casanovas[SUP] 7 [/SUP], Vassilis Skampardonis[SUP] 8 [/SUP], Julia Stadler[SUP] 9 [/SUP]
Affiliations
Abstract
Swine influenza A virus (swIAV), which plays a major role in the porcine respiratory disease complex (PRDC), is eliminated from the respiratory tract within 7-9 days after infection. Therefore, diagnosis is complicated in endemically infected swine herds presenting no obvious clinical signs. This study aimed to investigate the right time point for sampling to detect swIAV. A cross-sectional study was performed in 131 farms from 12 European countries. The sampling protocol included suckling piglets, weaners, and nursery pigs. In each age group, 10 nasal swabs were collected and further examined in pools of 5 for swIAV by Matrix rRT-PCR, followed by a multiplex RT-PCR to determine the influenza subtype. SwIAV was detected in 284 (37.9%) of the samples and on 103 (78.6%) farms. Despite the highest number of animals with clinical signs being found in the nursery, the weaners were significantly more often virus-positive compared to nursery pigs (p = 0.048). Overall, the swIAV detection rate did not significantly differ between diseased or non-diseased suckling and nursery piglets, respectively; however, diseased weaners had significantly more positive pools than the non-diseased animals. Interestingly, in 9 farms, different subtypes were detected in different age groups. Our findings indicate that to detect all circulating swIAV subtypes on a farm, different age groups should be sampled. Additionally, the sampling strategy should also aim to include non-diseased animals, especially in the suckling period.
Keywords: diagnosis; influenza A virus; nasal swabs; sampling; swine influenza.
. 2022 Jul 4;9(7):338.
doi: 10.3390/vetsci9070338.
A New Sampling Approach for the Detection of Swine Influenza a Virus on European Sow Farms
Kathrin Lillie-Jaschniski[SUP] 1 [/SUP], Marina Lisgara[SUP] 2 [/SUP], Emanuela Pileri[SUP] 3 [/SUP], Agnes Jardin[SUP] 4 [/SUP], Eduardo Velazquez[SUP] 5 [/SUP], Monika Köchling[SUP] 1 [/SUP], Michael Albin[SUP] 6 [/SUP], Carlos Casanovas[SUP] 7 [/SUP], Vassilis Skampardonis[SUP] 8 [/SUP], Julia Stadler[SUP] 9 [/SUP]
Affiliations
- PMID: 35878355
- DOI: 10.3390/vetsci9070338
Abstract
Swine influenza A virus (swIAV), which plays a major role in the porcine respiratory disease complex (PRDC), is eliminated from the respiratory tract within 7-9 days after infection. Therefore, diagnosis is complicated in endemically infected swine herds presenting no obvious clinical signs. This study aimed to investigate the right time point for sampling to detect swIAV. A cross-sectional study was performed in 131 farms from 12 European countries. The sampling protocol included suckling piglets, weaners, and nursery pigs. In each age group, 10 nasal swabs were collected and further examined in pools of 5 for swIAV by Matrix rRT-PCR, followed by a multiplex RT-PCR to determine the influenza subtype. SwIAV was detected in 284 (37.9%) of the samples and on 103 (78.6%) farms. Despite the highest number of animals with clinical signs being found in the nursery, the weaners were significantly more often virus-positive compared to nursery pigs (p = 0.048). Overall, the swIAV detection rate did not significantly differ between diseased or non-diseased suckling and nursery piglets, respectively; however, diseased weaners had significantly more positive pools than the non-diseased animals. Interestingly, in 9 farms, different subtypes were detected in different age groups. Our findings indicate that to detect all circulating swIAV subtypes on a farm, different age groups should be sampled. Additionally, the sampling strategy should also aim to include non-diseased animals, especially in the suckling period.
Keywords: diagnosis; influenza A virus; nasal swabs; sampling; swine influenza.