tetano
Editor, Senior Moderator
Vet Microbiol
. 2025 May 5:305:110546.
doi: 10.1016/j.vetmic.2025.110546. Online ahead of print. Recombinant Salmonella vector delivery 3M2e-ferritin fusion nanoparticles provide cross protection against H9N2 and H7N9 avian influenza viruses
Wangyangji Sun[SUP] 1 [/SUP], Yu-An Li[SUP] 2 [/SUP], Zewei Li[SUP] 3 [/SUP], Guohui Li[SUP] 4 [/SUP], Yuanzhao Du[SUP] 5 [/SUP], Huoying Shi[SUP] 6 [/SUP]
Affiliations
In recent years, the advancement of nanomedicine has revolutionized the field of vaccine development. Numerous nanoparticle-based nano vaccine candidates have been continuously developed. Herein, we describe a universal avian influenza virus (AIV) vaccine candidate based on ferritin self-assembling nanoparticles (NPs). The ferritin fused with 3M2e was delivered by an recombinant Salmonella vector, resulting in the formation of a novel recombinant strain, rSC0130(pYA3342-3M2eNPs). The 3M2e-ferritin fusion protein was efficiently expressed and self-assembled into NPs within the recombinant strain. The delivery of the recombinant strain rSC0130(pYA3342-3M2eNPs), which carries the 3M2e-ferritin fusion NPs, induced robust specific cellular and humoral immune responses against AIV in vivo, ultimately providing chickens with effective cross-protection against challenges from H9N2 and H7N9 avian influenza viruses. Our study underscores the potential of the Salmonella platform for delivering protein-based NPs and offers new insights into the development of universal vaccines for AIVs.
Keywords: 3M2e-ferritin fusion protein; H7N9 influenza virus; H9N2 influenza virus; Influenza virus; Salmonella vector.
. 2025 May 5:305:110546.
doi: 10.1016/j.vetmic.2025.110546. Online ahead of print. Recombinant Salmonella vector delivery 3M2e-ferritin fusion nanoparticles provide cross protection against H9N2 and H7N9 avian influenza viruses
Wangyangji Sun[SUP] 1 [/SUP], Yu-An Li[SUP] 2 [/SUP], Zewei Li[SUP] 3 [/SUP], Guohui Li[SUP] 4 [/SUP], Yuanzhao Du[SUP] 5 [/SUP], Huoying Shi[SUP] 6 [/SUP]
Affiliations
- PMID: 40339259
- DOI: 10.1016/j.vetmic.2025.110546
In recent years, the advancement of nanomedicine has revolutionized the field of vaccine development. Numerous nanoparticle-based nano vaccine candidates have been continuously developed. Herein, we describe a universal avian influenza virus (AIV) vaccine candidate based on ferritin self-assembling nanoparticles (NPs). The ferritin fused with 3M2e was delivered by an recombinant Salmonella vector, resulting in the formation of a novel recombinant strain, rSC0130(pYA3342-3M2eNPs). The 3M2e-ferritin fusion protein was efficiently expressed and self-assembled into NPs within the recombinant strain. The delivery of the recombinant strain rSC0130(pYA3342-3M2eNPs), which carries the 3M2e-ferritin fusion NPs, induced robust specific cellular and humoral immune responses against AIV in vivo, ultimately providing chickens with effective cross-protection against challenges from H9N2 and H7N9 avian influenza viruses. Our study underscores the potential of the Salmonella platform for delivering protein-based NPs and offers new insights into the development of universal vaccines for AIVs.
Keywords: 3M2e-ferritin fusion protein; H7N9 influenza virus; H9N2 influenza virus; Influenza virus; Salmonella vector.