tetano
Editor, Senior Moderator
Vaccines (Basel)
. 2021 Oct 17;9(10):1193.
doi: 10.3390/vaccines9101193.
Genetic Insights into the Middle East Respiratory Syndrome Coronavirus Infection among Saudi People
Hatem A Abuelizz[SUP] 1 [/SUP], Maha M AlRasheed[SUP] 2 [/SUP], Ali Alhoshani[SUP] 3 [/SUP], Tariq Alhawassi[SUP] 2 [/SUP]
Affiliations
Abstract
Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) was isolated for the first time in Saudi Arabia from a patient suffering from atypical pneumonia. The Saudi Genome database was built by King Abdulaziz Medical City via the next-generation sequencing of 7000 candidates.
Method: A large list of point mutations were reported in the region of the dipeptidyl peptidase 4 (DPP4) gene. The DPP4 amino acid residues correlated to MERS-CoV entry and the site of activity of DPP4 inhibitors was investigated. We retrieved the SNPs (Single-Nucleotide Polymorphism) with a variation frequency of >0.05.
Results: SNP 2:162,890,175 and SNP 2:162,891,848 in the intronic region were located within 50 bp of amino acid residues responsible for MERS-CoV entry, amino acids 259-296 and 205-258, respectively. The variation frequency of SNP 2:162,890,175 was 2321 out of 2379 screened individuals. Moreover, mutation of SNP 2:162,891,848, which is located near amino acid residues E205 and E206 (crucial for the activity of DPP4 inhibitors), occurred in 76 out of 2379 screened individuals.
Conclusions: Our study shows high variation frequency in the DPP4 region reported in the Saudi Genome database. The identified SNPs are of high significance for MERS-CoV infection in better understanding disease pathogenesis.
Keywords: DPP4; MERS-CoV; dipeptidyl peptidase 4; middle east respiratory syndrome.
. 2021 Oct 17;9(10):1193.
doi: 10.3390/vaccines9101193.
Genetic Insights into the Middle East Respiratory Syndrome Coronavirus Infection among Saudi People
Hatem A Abuelizz[SUP] 1 [/SUP], Maha M AlRasheed[SUP] 2 [/SUP], Ali Alhoshani[SUP] 3 [/SUP], Tariq Alhawassi[SUP] 2 [/SUP]
Affiliations
- PMID: 34696302
- DOI: 10.3390/vaccines9101193
Abstract
Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) was isolated for the first time in Saudi Arabia from a patient suffering from atypical pneumonia. The Saudi Genome database was built by King Abdulaziz Medical City via the next-generation sequencing of 7000 candidates.
Method: A large list of point mutations were reported in the region of the dipeptidyl peptidase 4 (DPP4) gene. The DPP4 amino acid residues correlated to MERS-CoV entry and the site of activity of DPP4 inhibitors was investigated. We retrieved the SNPs (Single-Nucleotide Polymorphism) with a variation frequency of >0.05.
Results: SNP 2:162,890,175 and SNP 2:162,891,848 in the intronic region were located within 50 bp of amino acid residues responsible for MERS-CoV entry, amino acids 259-296 and 205-258, respectively. The variation frequency of SNP 2:162,890,175 was 2321 out of 2379 screened individuals. Moreover, mutation of SNP 2:162,891,848, which is located near amino acid residues E205 and E206 (crucial for the activity of DPP4 inhibitors), occurred in 76 out of 2379 screened individuals.
Conclusions: Our study shows high variation frequency in the DPP4 region reported in the Saudi Genome database. The identified SNPs are of high significance for MERS-CoV infection in better understanding disease pathogenesis.
Keywords: DPP4; MERS-CoV; dipeptidyl peptidase 4; middle east respiratory syndrome.