tetano
Editor, Senior Moderator
Vaccines (Basel)
. 2022 Apr 8;10(4):578.
doi: 10.3390/vaccines10040578.
Dermal Delivery of a SARS-CoV-2 Subunit Vaccine Induces Immunogenicity against Variants of Concern
Christopher L D McMillan[SUP] 1 [/SUP], Armira Azuar[SUP] 1 [/SUP], Jovin J Y Choo[SUP] 1 [/SUP], Naphak Modhiran[SUP] 1 [/SUP], Alberto A Amarilla[SUP] 1 [/SUP], Ariel Isaacs[SUP] 1 [/SUP], Kate E Honeyman[SUP] 1 [/SUP], Stacey T M Cheung[SUP] 1 [/SUP], Benjamin Liang[SUP] 1 [/SUP], Maria J Wurm[SUP] 2 [/SUP], Paco Pino[SUP] 2 [/SUP], Joeri Kint[SUP] 2 [/SUP], Germain J P Fernando[SUP] 1 3 [/SUP], Michael J Landsberg[SUP] 1 4 [/SUP], Alexander A Khromykh[SUP] 1 4 [/SUP], Jody Hobson-Peters[SUP] 1 4 [/SUP], Daniel Watterson[SUP] 1 4 [/SUP], Paul R Young[SUP] 1 4 [/SUP], David A Muller[SUP] 1 4 [/SUP]
Affiliations
Abstract
The ongoing coronavirus disease 2019 (COVID-19) pandemic continues to disrupt essential health services in 90 percent of countries today. The spike (S) protein found on the surface of the causative agent, the SARS-CoV-2 virus, has been the prime target for current vaccine research since antibodies directed against the S protein were found to neutralize the virus. However, as new variants emerge, mutations within the spike protein have given rise to potential immune evasion of the response generated by the current generation of SARS-CoV-2 vaccines. In this study, a modified, HexaPro S protein subunit vaccine, delivered using a needle-free high-density microarray patch (HD-MAP), was investigated for its immunogenicity and virus-neutralizing abilities. Mice given two doses of the vaccine candidate generated potent antibody responses capable of neutralizing the parental SARS-CoV-2 virus as well as the variants of concern, Alpha and Delta. These results demonstrate that this alternative vaccination strategy has the potential to mitigate the effect of emerging viral variants.
Keywords: COVID-19; HD-MAP; HexaPro; S protein; SARS-CoV-2 vaccine; antibodies; cutaneous; intradermal; virus neutralization microneedle patch.
. 2022 Apr 8;10(4):578.
doi: 10.3390/vaccines10040578.
Dermal Delivery of a SARS-CoV-2 Subunit Vaccine Induces Immunogenicity against Variants of Concern
Christopher L D McMillan[SUP] 1 [/SUP], Armira Azuar[SUP] 1 [/SUP], Jovin J Y Choo[SUP] 1 [/SUP], Naphak Modhiran[SUP] 1 [/SUP], Alberto A Amarilla[SUP] 1 [/SUP], Ariel Isaacs[SUP] 1 [/SUP], Kate E Honeyman[SUP] 1 [/SUP], Stacey T M Cheung[SUP] 1 [/SUP], Benjamin Liang[SUP] 1 [/SUP], Maria J Wurm[SUP] 2 [/SUP], Paco Pino[SUP] 2 [/SUP], Joeri Kint[SUP] 2 [/SUP], Germain J P Fernando[SUP] 1 3 [/SUP], Michael J Landsberg[SUP] 1 4 [/SUP], Alexander A Khromykh[SUP] 1 4 [/SUP], Jody Hobson-Peters[SUP] 1 4 [/SUP], Daniel Watterson[SUP] 1 4 [/SUP], Paul R Young[SUP] 1 4 [/SUP], David A Muller[SUP] 1 4 [/SUP]
Affiliations
- PMID: 35455326
- PMCID: PMC9030474
- DOI: 10.3390/vaccines10040578
Abstract
The ongoing coronavirus disease 2019 (COVID-19) pandemic continues to disrupt essential health services in 90 percent of countries today. The spike (S) protein found on the surface of the causative agent, the SARS-CoV-2 virus, has been the prime target for current vaccine research since antibodies directed against the S protein were found to neutralize the virus. However, as new variants emerge, mutations within the spike protein have given rise to potential immune evasion of the response generated by the current generation of SARS-CoV-2 vaccines. In this study, a modified, HexaPro S protein subunit vaccine, delivered using a needle-free high-density microarray patch (HD-MAP), was investigated for its immunogenicity and virus-neutralizing abilities. Mice given two doses of the vaccine candidate generated potent antibody responses capable of neutralizing the parental SARS-CoV-2 virus as well as the variants of concern, Alpha and Delta. These results demonstrate that this alternative vaccination strategy has the potential to mitigate the effect of emerging viral variants.
Keywords: COVID-19; HD-MAP; HexaPro; S protein; SARS-CoV-2 vaccine; antibodies; cutaneous; intradermal; virus neutralization microneedle patch.