tetano
Editor, Senior Moderator
Vaccines (Basel)
. 2020 May 18;8(2):E229.
doi: 10.3390/vaccines8020229.
A Nanoparticle-Poly(I:C) Combination Adjuvant Enhances the Breadth of the Immune Response to Inactivated Influenza Virus Vaccine in Pigs
Sankar Renu[SUP] 1 2 [/SUP], Ninoshkaly Feliciano-Ruiz[SUP] 1 2 [/SUP], Fangjia Lu[SUP] 3 [/SUP], Shristi Ghimire[SUP] 1 2 [/SUP], Yi Han[SUP] 1 2 [/SUP], Jennifer Schrock[SUP] 1 2 [/SUP], Santosh Dhakal[SUP] 1 2 [/SUP], Veerupaxagouda Patil[SUP] 1 2 [/SUP], Steven Krakowka[SUP] 4 [/SUP], Harm HogenEsch[SUP] 3 [/SUP], Gourapura J Renukaradhya[SUP] 1 2 [/SUP]
Affiliations
Abstract
Intranasal vaccination elicits secretory IgA (SIgA) antibodies in the airways, which is required for cross-protection against influenza. To enhance the breadth of immunity induced by a killed swine influenza virus antigen (KAg) or conserved T cell and B cell peptides, we adsorbed the antigens together with the TLR3 agonist poly(I:C) electrostatically onto cationic alpha-D-glucan nanoparticles (Nano-11) resulting in Nano-11-KAg-poly(I:C) and Nano-11-peptides-poly(I:C) vaccines. In vitro, increased TNF-α and IL-1? cytokine mRNA expression was observed in Nano-11-KAg-poly(I:C)-treated porcine monocyte-derived dendritic cells. Nano-11-KAg-poly(I:C), but not Nano-11-peptides-poly(I:C), delivered intranasally in pigs induced high levels of cross-reactive virus-specific SIgA antibodies secretion in the nasal passage and lungs compared to a multivalent commercial influenza virus vaccine administered intramuscularly. The commercial and Nano-11-KAg-poly(I:C) vaccinations increased the frequency of IFNγ secreting T cells. The poly(I:C) adjuvanted Nano-11-based vaccines increased various cytokine mRNA expressions in lymph nodes compared to the commercial vaccine. In addition, Nano-11-KAg-poly(I:C) vaccine elicited high levels of virus neutralizing antibodies in bronchoalveolar lavage fluid. Microscopic lung lesions and challenge virus load were partially reduced in poly(I:C) adjuvanted Nano-11 and commercial influenza vaccinates. In conclusion, compared to our earlier study with Nano-11-KAg vaccine, addition of poly(I:C) to the formulation improved cross-protective antibody and cytokine response.
Keywords: Nano-11; T and B cell peptides; intranasal vaccination; mucosal immunity; pigs; poly(I:C); swine influenza virus.
. 2020 May 18;8(2):E229.
doi: 10.3390/vaccines8020229.
A Nanoparticle-Poly(I:C) Combination Adjuvant Enhances the Breadth of the Immune Response to Inactivated Influenza Virus Vaccine in Pigs
Sankar Renu[SUP] 1 2 [/SUP], Ninoshkaly Feliciano-Ruiz[SUP] 1 2 [/SUP], Fangjia Lu[SUP] 3 [/SUP], Shristi Ghimire[SUP] 1 2 [/SUP], Yi Han[SUP] 1 2 [/SUP], Jennifer Schrock[SUP] 1 2 [/SUP], Santosh Dhakal[SUP] 1 2 [/SUP], Veerupaxagouda Patil[SUP] 1 2 [/SUP], Steven Krakowka[SUP] 4 [/SUP], Harm HogenEsch[SUP] 3 [/SUP], Gourapura J Renukaradhya[SUP] 1 2 [/SUP]
Affiliations
- PMID: 32443416
- DOI: 10.3390/vaccines8020229
Abstract
Intranasal vaccination elicits secretory IgA (SIgA) antibodies in the airways, which is required for cross-protection against influenza. To enhance the breadth of immunity induced by a killed swine influenza virus antigen (KAg) or conserved T cell and B cell peptides, we adsorbed the antigens together with the TLR3 agonist poly(I:C) electrostatically onto cationic alpha-D-glucan nanoparticles (Nano-11) resulting in Nano-11-KAg-poly(I:C) and Nano-11-peptides-poly(I:C) vaccines. In vitro, increased TNF-α and IL-1? cytokine mRNA expression was observed in Nano-11-KAg-poly(I:C)-treated porcine monocyte-derived dendritic cells. Nano-11-KAg-poly(I:C), but not Nano-11-peptides-poly(I:C), delivered intranasally in pigs induced high levels of cross-reactive virus-specific SIgA antibodies secretion in the nasal passage and lungs compared to a multivalent commercial influenza virus vaccine administered intramuscularly. The commercial and Nano-11-KAg-poly(I:C) vaccinations increased the frequency of IFNγ secreting T cells. The poly(I:C) adjuvanted Nano-11-based vaccines increased various cytokine mRNA expressions in lymph nodes compared to the commercial vaccine. In addition, Nano-11-KAg-poly(I:C) vaccine elicited high levels of virus neutralizing antibodies in bronchoalveolar lavage fluid. Microscopic lung lesions and challenge virus load were partially reduced in poly(I:C) adjuvanted Nano-11 and commercial influenza vaccinates. In conclusion, compared to our earlier study with Nano-11-KAg vaccine, addition of poly(I:C) to the formulation improved cross-protective antibody and cytokine response.
Keywords: Nano-11; T and B cell peptides; intranasal vaccination; mucosal immunity; pigs; poly(I:C); swine influenza virus.