Giuseppe
Emeritus
Vaccine. 2009 Oct 23. [Epub ahead of print]
A novel assay for influenza virus quantification using surface plasmon resonance.
Nilsson CE, Abbas S, Bennemo M, Larsson A, H?m?l?inen MD, Frostell-Karlsson A. - GE Healthcare BioSciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden.
Quantification of hemagglutinin (HA) by single-radial immuno diffusion (SRID) is the predominant method to ensure product potency in seasonal influenza vaccines. Here a new method for quantification of influenza virus using biosensor technology is presented. The method employs quantification of virus via an inhibition assay format using HA proteins for H1N1, H3N2 and B immobilized on a sensor chip. Initial results showed the assay to have higher sensitivity (detection range 0.5-10mug/ml), higher precision and significantly lower analysis and hands on time compared with SRID.
PMID: 19857452 [PubMed - as supplied by publisher]
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A novel assay for influenza virus quantification using surface plasmon resonance.
Nilsson CE, Abbas S, Bennemo M, Larsson A, H?m?l?inen MD, Frostell-Karlsson A. - GE Healthcare BioSciences AB, Bjorkgatan 30, SE-751 84 Uppsala, Sweden.
Quantification of hemagglutinin (HA) by single-radial immuno diffusion (SRID) is the predominant method to ensure product potency in seasonal influenza vaccines. Here a new method for quantification of influenza virus using biosensor technology is presented. The method employs quantification of virus via an inhibition assay format using HA proteins for H1N1, H3N2 and B immobilized on a sensor chip. Initial results showed the assay to have higher sensitivity (detection range 0.5-10mug/ml), higher precision and significantly lower analysis and hands on time compared with SRID.
PMID: 19857452 [PubMed - as supplied by publisher]
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