tetano
Editor, Senior Moderator
Enferm Infecc Microbiol Clin. 2017 Jun 7. pii: S0213-005X(17)30141-6. doi: 10.1016/j.eimc.2017.04.008. [Epub ahead of print]
[h=1]Usefulness of Ct value in acute respiratory infections caused by respiratory syncytial virus A and B and influenza virus A (H1N1)pdm09, A (H3N2) and B.[/h] [Article in English, Spanish]
Reina J[SUP]1[/SUP], Morales C[SUP]2[/SUP], Busquets M[SUP]2[/SUP], Norte C[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]INTRODUCTION:[/h] Acute respiratory infections of viral cause are very frequent entities. The difficulty in evaluating the detection of a virus in these entities could be solved by determining the viral load.
[h=4]METHODS:[/h] A prospective study on the mean Ct value (cycle threshold value) detected against RSV-A, RSV-B and influenza A (H1N1)pdm09, A (H3N2) and B viruses in patients of different origin and age was performed. Detection was performed using a commercial molecular amplification (RT-PCR) technique.
[h=4]RESULTS:[/h] Different mean Ct values were detected for each virus. In RSV infections, no differences were observed between those caused by RSV-A or RSV-B in children. Depending on the patient's age, the only statistical significance was observed in those included in the 0-4 month groups for RSV-A and this group and the 5-12 months group for RSV-B (higher values). A lower viral load was detected in adult patients than in paediatric patients. In influenza infections, no statistical significance was observed in the mean values detected in patients from the Red Centinela (?sentinel network?, a Spanish network of doctors aimed at research and surveillance of diseases), those diagnosed in the adult emergency room or in hospital admissions. In the adult patients admitted to the ICU, only a slightly lower mean value was observed in those infected with influenza A (H1N1)pdm09, but without statistical significance. There were no patients admitted to the ICU with influenza B infection.
[h=4]CONCLUSION:[/h] The detection of viral load could be a good tool for the evaluation, monitoring and prognosis of acute viral respiratory infections. With the exception of those caused by RSV, no significant differences were observed in influenza infections except in younger paediatric patients.
Copyright ? 2017 Elsevier Espa?a, S.L.U. and Sociedad Espa?ola de Enfermedades Infecciosas y Microbiolog?a Cl?nica. All rights reserved.
[h=4]KEYWORDS:[/h] Acute respiratory infections; Carga viral; Ct value; Infecciones respiratorias agudas; Influenza viruses; Respiratory syncytial virus; Valor Ct; Viral load; Virus gripales; Virus respiratorio sincitial
PMID: 28601216 DOI: 10.1016/j.eimc.2017.04.008
[h=1]Usefulness of Ct value in acute respiratory infections caused by respiratory syncytial virus A and B and influenza virus A (H1N1)pdm09, A (H3N2) and B.[/h] [Article in English, Spanish]
Reina J[SUP]1[/SUP], Morales C[SUP]2[/SUP], Busquets M[SUP]2[/SUP], Norte C[SUP]2[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]INTRODUCTION:[/h] Acute respiratory infections of viral cause are very frequent entities. The difficulty in evaluating the detection of a virus in these entities could be solved by determining the viral load.
[h=4]METHODS:[/h] A prospective study on the mean Ct value (cycle threshold value) detected against RSV-A, RSV-B and influenza A (H1N1)pdm09, A (H3N2) and B viruses in patients of different origin and age was performed. Detection was performed using a commercial molecular amplification (RT-PCR) technique.
[h=4]RESULTS:[/h] Different mean Ct values were detected for each virus. In RSV infections, no differences were observed between those caused by RSV-A or RSV-B in children. Depending on the patient's age, the only statistical significance was observed in those included in the 0-4 month groups for RSV-A and this group and the 5-12 months group for RSV-B (higher values). A lower viral load was detected in adult patients than in paediatric patients. In influenza infections, no statistical significance was observed in the mean values detected in patients from the Red Centinela (?sentinel network?, a Spanish network of doctors aimed at research and surveillance of diseases), those diagnosed in the adult emergency room or in hospital admissions. In the adult patients admitted to the ICU, only a slightly lower mean value was observed in those infected with influenza A (H1N1)pdm09, but without statistical significance. There were no patients admitted to the ICU with influenza B infection.
[h=4]CONCLUSION:[/h] The detection of viral load could be a good tool for the evaluation, monitoring and prognosis of acute viral respiratory infections. With the exception of those caused by RSV, no significant differences were observed in influenza infections except in younger paediatric patients.
Copyright ? 2017 Elsevier Espa?a, S.L.U. and Sociedad Espa?ola de Enfermedades Infecciosas y Microbiolog?a Cl?nica. All rights reserved.
[h=4]KEYWORDS:[/h] Acute respiratory infections; Carga viral; Ct value; Infecciones respiratorias agudas; Influenza viruses; Respiratory syncytial virus; Valor Ct; Viral load; Virus gripales; Virus respiratorio sincitial
PMID: 28601216 DOI: 10.1016/j.eimc.2017.04.008