tetano
Editor, Senior Moderator
Transbound Emerg Dis
. 2020 Jul 8.
doi: 10.1111/tbed.13721. Online ahead of print.
Identification of Optimal Sample Collection Devices and Sampling Locations for the Detection Environmental Viral Contamination in Wire Poultry Cages
Jongseo Mo[SUP] 1 [/SUP], Erica Spackman[SUP] 1 [/SUP], Christopher B Stephens[SUP] 1 2 [/SUP]
Affiliations
Abstract
Environmental testing of poultry premises after an outbreak of an infectious disease like avian influenza (AI) or Newcastle disease, is essential to promptly verify virus-free status and subsequently return to normal operations. In an attempt to establish an optimized sampling protocol a laboratory study simulating an AI virus contaminated poultry house with wire layer cages was conducted. Three sample collection devices, pre-moistened cotton gauze, dry cotton gauze, and a foam swab, were evaluated with each of four sample locations within a cage and when sampling all four locations with one device. Virus was detected with quantitative real-time RT-PCR utilizing a standard curve of a quantified homologous isolate of AI virus to determine titer equivalents of virus. The pre-moistened gauze detected the most virus RNA (100% positive, geometric mean titer [GMT): 3.2 log[SUB]10[/SUB] 50% embryo infectious doses [EID[SUB]50[/SUB] ] equivalents per 25 cm[SUP]2[/SUP] ) in all four sample locations compared to dry gauze (93% positive, GMT: 2.6 EID[SUB]50[/SUB] equivalents per 25 cm[SUP]2[/SUP] ) and foam swabs (95% positive, GMT: 2.8 log10 EID[SUB]50[/SUB] equivalents per 25 cm[SUP]2[/SUP] ). The highest viral RNA loads were observed from the cage floor, and when the four locations were sampled with the same device. Overall, the pre-moistened gauze performed the best, and sampling multiple locations within a cage with the same device would likely optimize detection of residual virus.
Keywords: Avian influenza; Newcastle disease; Virus; environmental sampling; layer cage; wire cage.
. 2020 Jul 8.
doi: 10.1111/tbed.13721. Online ahead of print.
Identification of Optimal Sample Collection Devices and Sampling Locations for the Detection Environmental Viral Contamination in Wire Poultry Cages
Jongseo Mo[SUP] 1 [/SUP], Erica Spackman[SUP] 1 [/SUP], Christopher B Stephens[SUP] 1 2 [/SUP]
Affiliations
- PMID: 32643291
- DOI: 10.1111/tbed.13721
Abstract
Environmental testing of poultry premises after an outbreak of an infectious disease like avian influenza (AI) or Newcastle disease, is essential to promptly verify virus-free status and subsequently return to normal operations. In an attempt to establish an optimized sampling protocol a laboratory study simulating an AI virus contaminated poultry house with wire layer cages was conducted. Three sample collection devices, pre-moistened cotton gauze, dry cotton gauze, and a foam swab, were evaluated with each of four sample locations within a cage and when sampling all four locations with one device. Virus was detected with quantitative real-time RT-PCR utilizing a standard curve of a quantified homologous isolate of AI virus to determine titer equivalents of virus. The pre-moistened gauze detected the most virus RNA (100% positive, geometric mean titer [GMT): 3.2 log[SUB]10[/SUB] 50% embryo infectious doses [EID[SUB]50[/SUB] ] equivalents per 25 cm[SUP]2[/SUP] ) in all four sample locations compared to dry gauze (93% positive, GMT: 2.6 EID[SUB]50[/SUB] equivalents per 25 cm[SUP]2[/SUP] ) and foam swabs (95% positive, GMT: 2.8 log10 EID[SUB]50[/SUB] equivalents per 25 cm[SUP]2[/SUP] ). The highest viral RNA loads were observed from the cage floor, and when the four locations were sampled with the same device. Overall, the pre-moistened gauze performed the best, and sampling multiple locations within a cage with the same device would likely optimize detection of residual virus.
Keywords: Avian influenza; Newcastle disease; Virus; environmental sampling; layer cage; wire cage.