tetano
Editor, Senior Moderator
Zhonghua Yu Fang Yi Xue Za Zhi. 2010 Dec;44(12):1079-1082.
[The significance of different sample types in study of pandemic A(H1N1) influenza diagnosis.]
[Article in Chinese]
Huang F, Shi WX, Lu GL, Cui SJ, L? YN, Tian LL, Qian HK, Yang P, Wang QY, Pang XH.
Institute for Infectious Disease and Endemic Disease Control, Beijing Center of Disease Control and Prevention, Beijing 100031, China.
Abstract
OBJECTIVE: To explore the value of different types of samples, including throat swabs, stools, bloods in pandemic A (H1N1) influenza diagnosis and virus shedding patterns.
METHODS: From May to June in 2009, 135 samples were collected from 23 confirmed cases of pandemic influenza A (H1N1) infection, including 99 throat swabs, 14 stools, 11 bloods, 1 respiratory tract washing from 13 confirmed cases and 10 blood samples from other confirmed cases. The virus was detected by real-time RT-PCR, the antibody was detected by haemagglutination inhibition assay.
RESULTS: For 99 throat swabs of 13 patients, the median time of the first positive real-time RT-PCR was 1 day (ranged from 0 to 7 days) after the onset of the symptoms of illness; the median length of time duration of positive real-time RT-PCR results from throat swabs was 3 days (ranged from 1 to 15 days). Four cases intermittently released virus. One respiratory tract washing sample was positive. In 14 stools, 8 stools were real-time RT-PCR positive, the positive rate was 57.14%. The median time of the positive real-time RT-PCR was 3 days (ranged from 1 to 4 days) after the onset of the symptoms of illness. In 21 blood samples collected at 2 to 9 days of onset, 1 blood sample was real-time RT-PCR positive, the positive rate was 4.76%. All these 21 blood samples were antibody negative.
CONCLUSION: Throat swabs and stools samples can be used as A (H1N1) influenza early diagnosis. The length of time duration of positive real-time RT-PCR in throat swabs was longer than stool samples and intermittently releasing of virus were found in throat swabs. Influenza A H1N1 cases showed the presence of small amount of viremia and antibody was negative in early blood samples (< 9 days).
PMID: 21215107 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21215107
[The significance of different sample types in study of pandemic A(H1N1) influenza diagnosis.]
[Article in Chinese]
Huang F, Shi WX, Lu GL, Cui SJ, L? YN, Tian LL, Qian HK, Yang P, Wang QY, Pang XH.
Institute for Infectious Disease and Endemic Disease Control, Beijing Center of Disease Control and Prevention, Beijing 100031, China.
Abstract
OBJECTIVE: To explore the value of different types of samples, including throat swabs, stools, bloods in pandemic A (H1N1) influenza diagnosis and virus shedding patterns.
METHODS: From May to June in 2009, 135 samples were collected from 23 confirmed cases of pandemic influenza A (H1N1) infection, including 99 throat swabs, 14 stools, 11 bloods, 1 respiratory tract washing from 13 confirmed cases and 10 blood samples from other confirmed cases. The virus was detected by real-time RT-PCR, the antibody was detected by haemagglutination inhibition assay.
RESULTS: For 99 throat swabs of 13 patients, the median time of the first positive real-time RT-PCR was 1 day (ranged from 0 to 7 days) after the onset of the symptoms of illness; the median length of time duration of positive real-time RT-PCR results from throat swabs was 3 days (ranged from 1 to 15 days). Four cases intermittently released virus. One respiratory tract washing sample was positive. In 14 stools, 8 stools were real-time RT-PCR positive, the positive rate was 57.14%. The median time of the positive real-time RT-PCR was 3 days (ranged from 1 to 4 days) after the onset of the symptoms of illness. In 21 blood samples collected at 2 to 9 days of onset, 1 blood sample was real-time RT-PCR positive, the positive rate was 4.76%. All these 21 blood samples were antibody negative.
CONCLUSION: Throat swabs and stools samples can be used as A (H1N1) influenza early diagnosis. The length of time duration of positive real-time RT-PCR in throat swabs was longer than stool samples and intermittently releasing of virus were found in throat swabs. Influenza A H1N1 cases showed the presence of small amount of viremia and antibody was negative in early blood samples (< 9 days).
PMID: 21215107 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21215107