tetano
Editor, Senior Moderator
J Gen Virol. 2013 Jan 9. [Epub ahead of print]
The Role of Ran-Binding Protein 3 During Influenza A Virus Replication.
Predicala R, Zhou Y.
Source
University of Saskatchewan.
Abstract
Influenza A virus vRNP nuclear export is CRM1-dependent. Ran-binding protein 3 (BanBP3) is a Ran-interacting protein that is best known for its role as a cofactor of CRM1-mediated cargo nuclear export. In this study, we investigated the role of RanBP3 during influenza A virus life cycle. We found that RanBP3 was phosphorylated at Ser58 in early and late phase of infection. Knockdown of RanBP3 expression led to a vRNP nuclear retention, suggesting that RanBP3 is involved in vRNP nuclear export. Moreover, we demonstrated that RanBP3's function during vRNP nuclear export is regulated by phosphorylation at Ser58, and that RanBP3 phosphorylation is modulated by both PI3K/Akt and Ras/ERK/RSK pathways in the late phase of viral infection.
PMID:
23303829
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23303829
The Role of Ran-Binding Protein 3 During Influenza A Virus Replication.
Predicala R, Zhou Y.
Source
University of Saskatchewan.
Abstract
Influenza A virus vRNP nuclear export is CRM1-dependent. Ran-binding protein 3 (BanBP3) is a Ran-interacting protein that is best known for its role as a cofactor of CRM1-mediated cargo nuclear export. In this study, we investigated the role of RanBP3 during influenza A virus life cycle. We found that RanBP3 was phosphorylated at Ser58 in early and late phase of infection. Knockdown of RanBP3 expression led to a vRNP nuclear retention, suggesting that RanBP3 is involved in vRNP nuclear export. Moreover, we demonstrated that RanBP3's function during vRNP nuclear export is regulated by phosphorylation at Ser58, and that RanBP3 phosphorylation is modulated by both PI3K/Akt and Ras/ERK/RSK pathways in the late phase of viral infection.
PMID:
23303829
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23303829