tetano
Editor, Senior Moderator
Int J Mol Sci. 2018 Jun 26;19(7). pii: E1877. doi: 10.3390/ijms19071877.
[h=1]The RNA Helicase DDX6 Associates with RIG-I to Augment Induction of Antiviral Signaling.[/h] N??ez RD[SUP]1[/SUP], Budt M[SUP]2[/SUP], Saenger S[SUP]3[/SUP], Paki K[SUP]4[/SUP], Arnold U[SUP]5[/SUP], Sadewasser A[SUP]6[/SUP], Wolff T[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Virus infections induce sensitive antiviral responses within the host cell. The RNA helicase retinoic acid-inducible gene I (RIG-I) is a key sensor of influenza virus RNA that induces the expression of antiviral type I interferons. Recent evidence suggests a complex pattern of RIG-I regulation involving multiple interactions and cellular sites. In an approach employing affinity purification and quantitative mass spectrometry, we identified proteins with increased binding to RIG-I in response to influenza B virus infection. Among them was the RIG-I related RNA helicase DEAD box helicase 6 (DDX6), a known component of cytoplasmic mRNA-ribonucleoprotein (mRNP) granules like P-bodies and stress granules (SGs). RIG-I and DDX6 both localized to the cytosol and were detected in virus-induced SGs. Coimmunoprecipitation assays detected a basal level of complexes harboring RIG-I and DDX6 that increased after infection. Functionally, DDX6 augmented RIG-I mediated induction of interferon (IFN)-β expression. Notably, DDX6 was found to bind viral RNA capable to stimulate RIG-I. These findings imply a novel function for DDX6 as an RNA co-sensor and signaling enhancer for RIG-I.
[h=4]KEYWORDS:[/h] DDX6; RIG-I; influenza; interferon
PMID: 29949917 DOI: 10.3390/ijms19071877
[h=1]The RNA Helicase DDX6 Associates with RIG-I to Augment Induction of Antiviral Signaling.[/h] N??ez RD[SUP]1[/SUP], Budt M[SUP]2[/SUP], Saenger S[SUP]3[/SUP], Paki K[SUP]4[/SUP], Arnold U[SUP]5[/SUP], Sadewasser A[SUP]6[/SUP], Wolff T[SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Virus infections induce sensitive antiviral responses within the host cell. The RNA helicase retinoic acid-inducible gene I (RIG-I) is a key sensor of influenza virus RNA that induces the expression of antiviral type I interferons. Recent evidence suggests a complex pattern of RIG-I regulation involving multiple interactions and cellular sites. In an approach employing affinity purification and quantitative mass spectrometry, we identified proteins with increased binding to RIG-I in response to influenza B virus infection. Among them was the RIG-I related RNA helicase DEAD box helicase 6 (DDX6), a known component of cytoplasmic mRNA-ribonucleoprotein (mRNP) granules like P-bodies and stress granules (SGs). RIG-I and DDX6 both localized to the cytosol and were detected in virus-induced SGs. Coimmunoprecipitation assays detected a basal level of complexes harboring RIG-I and DDX6 that increased after infection. Functionally, DDX6 augmented RIG-I mediated induction of interferon (IFN)-β expression. Notably, DDX6 was found to bind viral RNA capable to stimulate RIG-I. These findings imply a novel function for DDX6 as an RNA co-sensor and signaling enhancer for RIG-I.
[h=4]KEYWORDS:[/h] DDX6; RIG-I; influenza; interferon
PMID: 29949917 DOI: 10.3390/ijms19071877