tetano
Editor, Senior Moderator
FEBS Open Bio. 2017 Nov 6;7(12):1960-1971. doi: 10.1002/2211-5463.12336. eCollection 2017 Dec.
[h=1]The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labeling.[/h] Tsai CF[SUP]1[/SUP], Lin HY[SUP]1[/SUP], Hsu WL[SUP]2[/SUP], Tsai CH[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The nonstructural protein 1 (NS1) of the influenza A virus (IAV) is a multifunctional protein that counteracts host cell antiviral responses and inhibits host cell pre-mRNA processing. NS1 contains two nuclear localization signals that facilitate NS1 shuttling between cytoplasm and nucleus. In this study, we initially observed the novel mitochondria localization of NS1 in a subset of transfected cells. We then further monitored the localization dynamics of the NS1 protein in live cells infected with IAV expressing NS1 with insertion of a tetracysteine-tag. The resulting mutant virus showed similar levels of infectivity and expression pattern of NS1 to those of wild-type IAV. Pulse labeling using a biarsenical compound (fluorescein arsenical hairpin binder) allowed us to visualize the dynamic subcellular distribution of NS1 real time. We detected NS1 in mitochondria at a very early infection time point [1.5 h postinfection (hpi)] and observed the formation of a granular structure pattern in the nucleus at 4 hpi. This is the first identification of the novel mitochondria localization of NS1. The possible role of NS1 at an early infection time point is discussed.
[h=4]KEYWORDS:[/h] NS1 protein; influenza A virus; mitochondria localization; tetracysteine‐tag
PMID: 29226082 PMCID: PMC5715299 DOI: 10.1002/2211-5463.12336
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[h=1]The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labeling.[/h] Tsai CF[SUP]1[/SUP], Lin HY[SUP]1[/SUP], Hsu WL[SUP]2[/SUP], Tsai CH[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The nonstructural protein 1 (NS1) of the influenza A virus (IAV) is a multifunctional protein that counteracts host cell antiviral responses and inhibits host cell pre-mRNA processing. NS1 contains two nuclear localization signals that facilitate NS1 shuttling between cytoplasm and nucleus. In this study, we initially observed the novel mitochondria localization of NS1 in a subset of transfected cells. We then further monitored the localization dynamics of the NS1 protein in live cells infected with IAV expressing NS1 with insertion of a tetracysteine-tag. The resulting mutant virus showed similar levels of infectivity and expression pattern of NS1 to those of wild-type IAV. Pulse labeling using a biarsenical compound (fluorescein arsenical hairpin binder) allowed us to visualize the dynamic subcellular distribution of NS1 real time. We detected NS1 in mitochondria at a very early infection time point [1.5 h postinfection (hpi)] and observed the formation of a granular structure pattern in the nucleus at 4 hpi. This is the first identification of the novel mitochondria localization of NS1. The possible role of NS1 at an early infection time point is discussed.
[h=4]KEYWORDS:[/h] NS1 protein; influenza A virus; mitochondria localization; tetracysteine‐tag
PMID: 29226082 PMCID: PMC5715299 DOI: 10.1002/2211-5463.12336
Free full text