tetano
Editor, Senior Moderator
Virology. 2013 Dec;447(1-2):1-8. doi: 10.1016/j.virol.2013.08.022. Epub 2013 Sep 13.
The multifaceted effect of PB1-F2 specific antibodies on influenza A virus infection.
Ko??k I, Krejnusov? I, Pr?znovsk? M, Russ G.
Source
Institute of Virology, Slovak Academy of Sciences, D?bravsk? cesta 9, 845 05 Bratislava, Slovak Republic.
Abstract
PB1-F2 is a small influenza A virus (IAV) protein encoded by an alternative reading frame of the PB1 gene. During IAV infection, antibodies to PB1-F2 proteins are induced. To determine their function and contribution to virus infection, three distinct approaches were employed: passive transfer of anti-PB1-F2 MAbs and polyclonal antibodies, active immunization with PB1-F2 peptides and DNA vaccination with plasmids expressing various parts of PB1-F2. Mostly N-terminal specific antibodies were detected in polyclonal sera raised to complete PB1-F2. Passive and active immunization revealed that antibodies recognizing the N-terminal part of the PB1-F2 molecule have no remarkable effect on the course of IAV infection. Interestingly antibodies against the C-terminal region of PB1-F2, obtained by immunization with KLH-PB1-F2 C-terminal peptide or DNA immunization with pC-ter.PB1-F2 plasmid, partially protected mice against virus infection. To our knowledge, this is the first report demonstrating the biological relevance of humoral immunity against PB1-F2 protein in vivo.
? 2013 Elsevier Inc. All rights reserved.
KEYWORDS:
Antibodies, DNA vaccination, Humoral immunity, Immunogenicity, Influenza, PB1-F2
PMID:
24210094
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24210094
The multifaceted effect of PB1-F2 specific antibodies on influenza A virus infection.
Ko??k I, Krejnusov? I, Pr?znovsk? M, Russ G.
Source
Institute of Virology, Slovak Academy of Sciences, D?bravsk? cesta 9, 845 05 Bratislava, Slovak Republic.
Abstract
PB1-F2 is a small influenza A virus (IAV) protein encoded by an alternative reading frame of the PB1 gene. During IAV infection, antibodies to PB1-F2 proteins are induced. To determine their function and contribution to virus infection, three distinct approaches were employed: passive transfer of anti-PB1-F2 MAbs and polyclonal antibodies, active immunization with PB1-F2 peptides and DNA vaccination with plasmids expressing various parts of PB1-F2. Mostly N-terminal specific antibodies were detected in polyclonal sera raised to complete PB1-F2. Passive and active immunization revealed that antibodies recognizing the N-terminal part of the PB1-F2 molecule have no remarkable effect on the course of IAV infection. Interestingly antibodies against the C-terminal region of PB1-F2, obtained by immunization with KLH-PB1-F2 C-terminal peptide or DNA immunization with pC-ter.PB1-F2 plasmid, partially protected mice against virus infection. To our knowledge, this is the first report demonstrating the biological relevance of humoral immunity against PB1-F2 protein in vivo.
? 2013 Elsevier Inc. All rights reserved.
KEYWORDS:
Antibodies, DNA vaccination, Humoral immunity, Immunogenicity, Influenza, PB1-F2
PMID:
24210094
[PubMed - in process]
http://www.ncbi.nlm.nih.gov/pubmed/24210094