tetano
Editor, Senior Moderator
Scand J Immunol. 2016 Oct 8. doi: 10.1111/sji.12496. [Epub ahead of print]
[h=1]The influenza A virus nonstructural protein NS1 up-regulates the expression of collagen triple helix repeat containing 1 protein.[/h] Zhu C[SUP]1,[/SUP][SUP]2[/SUP], Peng G[SUP]1[/SUP], Yi W[SUP]2[/SUP], Song H[SUP]3[/SUP], Liu F[SUP]4[/SUP], Liu X[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) infection induces a strong immune response and regulates the expression of many host proteins. The collagen triple helix repeat containing 1 (CTHRC1) protein is a secreted protein that exhibits increased expression during the viral infection process. However, the regulatory function of IAV on CTHRC1 expression is obscure. In this study, we investigated the effect of IAV on CTHRC1 expression and its regulatory mechanism. A total of 106 serum specimens from healthy people and 80 serum specimens from patients infected with IAV were collected. The CTHRC1 levels in the sera from the IVA patients and healthy individuals were measured using an enzyme-linked immunosorbent assay (ELISA), and the differences were statistically analyzed. A549 cells were infected with the IAV or delNS1 virus. Additionally, A549 cells were co-transfected with a eukaryotic nonstructural NS1 protein gene expression plasmid and the CTHRC1 gene promoter reporter plasmid (pCTHRC1-Luc), the luciferase activities were assessed. The CTHRC1 mRNA and protein expression were detected using reverse transcription polymerase chain reaction (RT-PCR) and western blotting, respectively. The serum CTHRC1 level was significantly higher in the IAV patients than in the healthy individuals. IAV up-regulated the CTHRC1 mRNA and protein expression. The nonstructural NS1 protein specifically activated CTHRC1 gene promoter activity and up-regulated CTHRC1 mRNA and protein expression. The activation function had a dose-dependent effect, indicating that influenza virus up-regulated CTHRC1 expression through its NS1 protein. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A virus; collagen triple helix repeat containing 1; nonstructural protein NS1
PMID: 27718266 DOI: 10.1111/sji.12496
[PubMed - as supplied by publisher]
[h=1]The influenza A virus nonstructural protein NS1 up-regulates the expression of collagen triple helix repeat containing 1 protein.[/h] Zhu C[SUP]1,[/SUP][SUP]2[/SUP], Peng G[SUP]1[/SUP], Yi W[SUP]2[/SUP], Song H[SUP]3[/SUP], Liu F[SUP]4[/SUP], Liu X[SUP]5[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A virus (IAV) infection induces a strong immune response and regulates the expression of many host proteins. The collagen triple helix repeat containing 1 (CTHRC1) protein is a secreted protein that exhibits increased expression during the viral infection process. However, the regulatory function of IAV on CTHRC1 expression is obscure. In this study, we investigated the effect of IAV on CTHRC1 expression and its regulatory mechanism. A total of 106 serum specimens from healthy people and 80 serum specimens from patients infected with IAV were collected. The CTHRC1 levels in the sera from the IVA patients and healthy individuals were measured using an enzyme-linked immunosorbent assay (ELISA), and the differences were statistically analyzed. A549 cells were infected with the IAV or delNS1 virus. Additionally, A549 cells were co-transfected with a eukaryotic nonstructural NS1 protein gene expression plasmid and the CTHRC1 gene promoter reporter plasmid (pCTHRC1-Luc), the luciferase activities were assessed. The CTHRC1 mRNA and protein expression were detected using reverse transcription polymerase chain reaction (RT-PCR) and western blotting, respectively. The serum CTHRC1 level was significantly higher in the IAV patients than in the healthy individuals. IAV up-regulated the CTHRC1 mRNA and protein expression. The nonstructural NS1 protein specifically activated CTHRC1 gene promoter activity and up-regulated CTHRC1 mRNA and protein expression. The activation function had a dose-dependent effect, indicating that influenza virus up-regulated CTHRC1 expression through its NS1 protein. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Influenza A virus; collagen triple helix repeat containing 1; nonstructural protein NS1
PMID: 27718266 DOI: 10.1111/sji.12496
[PubMed - as supplied by publisher]