tetano
Editor, Senior Moderator
Hum Immunol. 2013 Jan 4. pii: S0198-8859(12)00690-8. doi: 10.1016/j.humimm.2012.12.016. [Epub ahead of print]
The functional CD8 T cell memory recall repertoire responding to the influenza A M1(58-66) epitope is polyclonal and shows a complex clonotype distribution.
Zhou V, Yassai MB, Regunathan J, Box J, Bosenko D, Vashishath Y, Demos W, Gorski J.
Source
Blood Research Institute BloodCenter of Wisconsin Milwaukee WI 53201.
Abstract
The CD8 memory T cell repertoire to the influenza A derived M1(58-66) epitope shows a restricted V genes and CDR3 sequences usage. The repertoire is highly polyclonal and the clonotype distribution has been described as consisting of two components, one showing a power law-like distribution and the other composed of a few clonotypes with a very high relative frequency. The question is whether the complex repertoire defined by its ability to flourish in a short term recall culture corresponded to functional cells. Here we show that there is a relation between expression of the degranulation marker CD107 and cytotoxicity or IFN-γ production in CD8 T cell lines and clones. We then examine recently degranulated CD8 cells from recall cultures from four middle aged HLA-A2 subjects and show that these functional cells are polyclonal. The clonotype distributions of the CD8 + CD107+ repertoires are complex in the same manner as previously reported. The clonotype composition of CD8 + CD107+ repertoires is also very similar to CD8 only repertoires, and to CD8 + HLA-A2-M1(58-66) pentamer positive repertoires. We postulate that multiple exposures during childhood to this conserved influenza A epitope has generated a complex functional repertoire in HLA-A2 individuals.
Copyright ? 2013. Published by Elsevier Inc.
PMID:
23295548
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23295548
The functional CD8 T cell memory recall repertoire responding to the influenza A M1(58-66) epitope is polyclonal and shows a complex clonotype distribution.
Zhou V, Yassai MB, Regunathan J, Box J, Bosenko D, Vashishath Y, Demos W, Gorski J.
Source
Blood Research Institute BloodCenter of Wisconsin Milwaukee WI 53201.
Abstract
The CD8 memory T cell repertoire to the influenza A derived M1(58-66) epitope shows a restricted V genes and CDR3 sequences usage. The repertoire is highly polyclonal and the clonotype distribution has been described as consisting of two components, one showing a power law-like distribution and the other composed of a few clonotypes with a very high relative frequency. The question is whether the complex repertoire defined by its ability to flourish in a short term recall culture corresponded to functional cells. Here we show that there is a relation between expression of the degranulation marker CD107 and cytotoxicity or IFN-γ production in CD8 T cell lines and clones. We then examine recently degranulated CD8 cells from recall cultures from four middle aged HLA-A2 subjects and show that these functional cells are polyclonal. The clonotype distributions of the CD8 + CD107+ repertoires are complex in the same manner as previously reported. The clonotype composition of CD8 + CD107+ repertoires is also very similar to CD8 only repertoires, and to CD8 + HLA-A2-M1(58-66) pentamer positive repertoires. We postulate that multiple exposures during childhood to this conserved influenza A epitope has generated a complex functional repertoire in HLA-A2 individuals.
Copyright ? 2013. Published by Elsevier Inc.
PMID:
23295548
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23295548