tetano
Editor, Senior Moderator
Talanta
. 2022 Jan 4;240:123209.
doi: 10.1016/j.talanta.2022.123209. Online ahead of print.
Recombinase polymerase amplification integrated with microfluidics for nucleic acid testing at point of care
Yuemeng Bai[SUP] 1 [/SUP], Jingcheng Ji[SUP] 1 [/SUP], Fengdan Ji[SUP] 1 [/SUP], Shuang Wu[SUP] 2 [/SUP], Yuan Tian[SUP] 1 [/SUP], Birui Jin[SUP] 3 [/SUP], Zedong Li[SUP] 4 [/SUP]
Affiliations
Abstract
Nucleic acid testing (NAT) implemented on a portable, miniaturized, and integrated device with rapid and sensitive results readout is highly demanded for pathogen detection or genetic screening at resource-limited settings, especially after the outbreak of coronavirus disease 2019 (COVID-19). The integration of recombinase polymerase amplification (RPA) with emerging microfluidics, classified by paper-based microfluidics and chip-based microfluidics, shows great potential to perform laboratory independent NAT assays at point of care with minimal labor, time and energy consumption. This review summarizes the state-of-the-art of RPA integrated with paper-based microfluidics and chip-based microfluidics, and discusses their pros and cons. Finally, existing challenges and possible ways for optimization of microfluidics-based RPA are proposed.
Keywords: Digital RPA; Isothermal amplification; Paper microfluidics; Pathogen detection.
. 2022 Jan 4;240:123209.
doi: 10.1016/j.talanta.2022.123209. Online ahead of print.
Recombinase polymerase amplification integrated with microfluidics for nucleic acid testing at point of care
Yuemeng Bai[SUP] 1 [/SUP], Jingcheng Ji[SUP] 1 [/SUP], Fengdan Ji[SUP] 1 [/SUP], Shuang Wu[SUP] 2 [/SUP], Yuan Tian[SUP] 1 [/SUP], Birui Jin[SUP] 3 [/SUP], Zedong Li[SUP] 4 [/SUP]
Affiliations
- PMID: 35026642
- DOI: 10.1016/j.talanta.2022.123209
Abstract
Nucleic acid testing (NAT) implemented on a portable, miniaturized, and integrated device with rapid and sensitive results readout is highly demanded for pathogen detection or genetic screening at resource-limited settings, especially after the outbreak of coronavirus disease 2019 (COVID-19). The integration of recombinase polymerase amplification (RPA) with emerging microfluidics, classified by paper-based microfluidics and chip-based microfluidics, shows great potential to perform laboratory independent NAT assays at point of care with minimal labor, time and energy consumption. This review summarizes the state-of-the-art of RPA integrated with paper-based microfluidics and chip-based microfluidics, and discusses their pros and cons. Finally, existing challenges and possible ways for optimization of microfluidics-based RPA are proposed.
Keywords: Digital RPA; Isothermal amplification; Paper microfluidics; Pathogen detection.