tetano
Editor, Senior Moderator
Talanta
. 2021 Dec 31;240:123197.
doi: 10.1016/j.talanta.2021.123197. Online ahead of print.
Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection
Jianwei Gao[SUP] 1 [/SUP], Chunhua Wang[SUP] 2 [/SUP], Yujin Chu[SUP] 3 [/SUP], Yingkuan Han[SUP] 4 [/SUP], Yakun Gao[SUP] 5 [/SUP], Yanhao Wang[SUP] 6 [/SUP], Chao Wang[SUP] 7 [/SUP], Hong Liu[SUP] 8 [/SUP], Lin Han[SUP] 9 [/SUP], Yu Zhang[SUP] 10 [/SUP]
Affiliations
Abstract
The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through π-π stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as ∼8 fg/mL, which shows ∼3 × sensitivity enhancement compared with Gr-FET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.
Keywords: Graphene field-effect transistor; Protein detection; SARS-CoV-2; Van der waals heterostructure.
. 2021 Dec 31;240:123197.
doi: 10.1016/j.talanta.2021.123197. Online ahead of print.
Graphene oxide-graphene Van der Waals heterostructure transistor biosensor for SARS-CoV-2 protein detection
Jianwei Gao[SUP] 1 [/SUP], Chunhua Wang[SUP] 2 [/SUP], Yujin Chu[SUP] 3 [/SUP], Yingkuan Han[SUP] 4 [/SUP], Yakun Gao[SUP] 5 [/SUP], Yanhao Wang[SUP] 6 [/SUP], Chao Wang[SUP] 7 [/SUP], Hong Liu[SUP] 8 [/SUP], Lin Han[SUP] 9 [/SUP], Yu Zhang[SUP] 10 [/SUP]
Affiliations
- PMID: 34996016
- PMCID: PMC8719368
- DOI: 10.1016/j.talanta.2021.123197
Abstract
The current outbreaking of the coronavirus SARS-CoV-2 pandemic threatens global health and has caused serious concern. Currently there is no specific drug against SARS-CoV-2, therefore, a fast and accurate diagnosis method is an urgent need for the diagnosis, timely treatment and infection control of COVID-19 pandemic. In this work, we developed a field effect transistor (FET) biosensor based on graphene oxide-graphene (GO/Gr) van der Waals heterostructure for selective and ultrasensitive SARS-CoV-2 proteins detection. The GO/Gr van der Waals heterostructure was in-situ formed in the microfluidic channel through π-π stacking. The developed biosensor is capable of SARS-CoV-2 proteins detection within 20 min in the large dynamic range from 10 fg/mL to 100 pg/mL with the limit of detection of as low as ∼8 fg/mL, which shows ∼3 × sensitivity enhancement compared with Gr-FET biosensor. The performance enhancement mechanism was studied based on the transistor-based biosensing theory and experimental results, which is mainly attributed to the enhanced SARS-CoV-2 capture antibody immobilization density due to the introduction of the GO layer on the graphene surface. The spiked SARS-CoV-2 protein samples in throat swab buffer solution were tested to confirm the practical application of the biosensor for SARS-CoV-2 proteins detection. The results indicated that the developed GO/Gr van der Waals heterostructure FET biosensor has strong selectivity and high sensitivity, providing a potential method for SARS-CoV-2 fast and accurate detection.
Keywords: Graphene field-effect transistor; Protein detection; SARS-CoV-2; Van der waals heterostructure.