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Susceptibility of swine cells and domestic pigs to SARS-CoV-2
David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Dan iel W. Madden, Heinz Feldmann, Jamie Henningson, Wenju n Ma, Udeni B. R. Balasuriya, Juergen A. Richt
doi: https://doi.org/10.1101/2020.08.15.252395
This article is a preprint and has not been certified by peer review [what does this mean?].
Abstract
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naive sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.
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https://www.biorxiv.org/content/10.1...08.15.252395v1
David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Dan iel W. Madden, Heinz Feldmann, Jamie Henningson, Wenju n Ma, Udeni B. R. Balasuriya, Juergen A. Richt
doi: https://doi.org/10.1101/2020.08.15.252395
This article is a preprint and has not been certified by peer review [what does this mean?].
Abstract
The emergence of SARS-CoV-2 has resulted in an ongoing global pandemic with significant morbidity, mortality, and economic consequences. The susceptibility of different animal species to SARS-CoV-2 is of concern due to the potential for interspecies transmission, and the requirement for pre-clinical animal models to develop effective countermeasures. In the current study, we determined the ability of SARS-CoV-2 to (i) replicate in porcine cell lines, (ii) establish infection in domestic pigs via experimental oral/intranasal/intratracheal inoculation, and (iii) transmit to co-housed naive sentinel pigs. SARS-CoV-2 was able to replicate in two different porcine cell lines with cytopathic effects. Interestingly, none of the SARS-CoV-2-inoculated pigs showed evidence of clinical signs, viral replication or SARS-CoV-2-specific antibody responses. Moreover, none of the sentinel pigs displayed markers of SARS-CoV-2 infection. These data indicate that although different porcine cell lines are permissive to SARS-CoV-2, five-week old pigs are not susceptible to infection via oral/intranasal/intratracheal challenge. Pigs are therefore unlikely to be significant carriers of SARS-CoV-2 and are not a suitable pre-clinical animal model to study SARS-CoV-2 pathogenesis or efficacy of respective vaccines or therapeutics.
...
https://www.biorxiv.org/content/10.1...08.15.252395v1