tetano
Editor, Senior Moderator
STAR Protoc
. 2021 Dec 8;101067.
doi: 10.1016/j.xpro.2021.101067. Online ahead of print.
Detection of N6-methyladenosine in SARS-CoV-2 RNA by methylated RNA immunoprecipitation sequencing (MeRIP-Seq)
Na Li[SUP] 1 [/SUP], Tariq Rana[SUP] 1 2 [/SUP]
Affiliations
Abstract
N [SUP]6[/SUP] -methylation of adenosine (m6A) is the most abundant internal mRNA modification and is an important post-transcriptional regulator of gene expression. Here, we describe a protocol for methylated RNA immunoprecipitation sequencing (MeRIP-Seq) to detect and quantify m6A-modifications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The protocol is optimized for low viral RNA levels and is readily adaptable for other applications.
. 2021 Dec 8;101067.
doi: 10.1016/j.xpro.2021.101067. Online ahead of print.
Detection of N6-methyladenosine in SARS-CoV-2 RNA by methylated RNA immunoprecipitation sequencing (MeRIP-Seq)
Na Li[SUP] 1 [/SUP], Tariq Rana[SUP] 1 2 [/SUP]
Affiliations
- PMID: 34901888
- PMCID: PMC8651518
- DOI: 10.1016/j.xpro.2021.101067
Abstract
N [SUP]6[/SUP] -methylation of adenosine (m6A) is the most abundant internal mRNA modification and is an important post-transcriptional regulator of gene expression. Here, we describe a protocol for methylated RNA immunoprecipitation sequencing (MeRIP-Seq) to detect and quantify m6A-modifications in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA. The protocol is optimized for low viral RNA levels and is readily adaptable for other applications.