Mingus
Well-known member
This Thai scientific seem to be a reference on that matter.
http://rongsriyam.lib.bioinfo.pl/auth:Rongsriyam,Y
_______________________________
Latest Paper:
<table class="css_list" id="pmid_17124984" name="pmid_17124984" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2006 Mar ;37(2):265-71 17124984 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Formulation of tablets from the crude extract of Rhinacanthus nasutus (Thai local plant) against Aedes aegypti and Culex quinquefasciatus larvae: a preliminary study. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yupha Rongsriyam , Yuwadee Trongtokit , Narumon Komalamisra , Nuttanan Sinchaipanich , Chamnarn Apiwathnasorn , Aumpol Mitrejet </td></tr> <tr> <td colspan="2">Dried root powder of Rhinacanthus nasutus, Thong Phan Chang (Thai name) were extracted with methanol (MeOH) in a Soxhlet apparatus and made into 2 formulations of tablet containing the extract at 5% and 10% concentration. Due to the viscous and poor flow properties of the crude MeOH extract obtained, a wet granulation method was conducted in developing the tablets. Lactose was used as a filler. Polyvinyl pyrrolidone (PVP) K30 (15% w/w solution in alcohol) was used as the binding agent, while stearic acid (2% w/w) was used as a lubricant. Both formulas of prepared tablets had a smooth shiny surface with a round shape. Other physical properties of the tablets, such as weight variation, friability and disintegration time, met the requirements of the USP XX standard. The mosquito larvicidal activity of prepared tablets containing 5% and 10% R. nasutus extract against Aedes aegypti were not significantly different from each other (p > 0.05), with 48-hour LC50 values of 13.6 and 14.2 mg/I for the 5% and 10% tablets, respectively, while their activities against Culex quinquefasciatus were similar (p > 0.05) with LC50 values of 18.7 and 17.3, respectively. The larvicidal activity levels against Ae. aegypti and Cx. quinquefasciatus were also not significantly different from each other (p > 0.05). No larval mortality was observed in the two control groups: lactose solution and dechlorinated water. Toxicity to female and male fish (Poecilia reticulata) was tested with the prepared tablets. The toxicity of tablets containing 5% and 10% extracts were not significantly different from each other for the P. reticulata females with 48-hour LC50 values of 105.2 and 110.8 mg/I, respectively, and for P. reticulata males with LC50 values of 99.1 and 103.4 mg/I, respectively. Female and male P. reticulata were sensitive to the same dose of the extract. No fish died in the two control groups, with lactose solution and dechlorinated water. Acute-toxicity bioassay with fish showed that with an exposure of 48 hours the LC50 values of the tablets containing 5% and 10% were 5- to 10-fold higher than the LC50 of R. nasutus against mosquito larvae. These prepared tablets could possibly used to control mosquito vectors and be introduced into the mosquito control program. </td></tr></tbody></table> <table class="css_list" id="pmid_16610644" name="pmid_16610644" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 Nov ;36:1423-31 16610644 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Efficacy of repellent products against caged and free flying Anopheles stephensi mosquitoes. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Christopher F Curtis , Yupha Rongsriyam </td></tr> <tr> <td colspan="2">The efficacy of 9 repellents (8 commercial repellents and one product under development) was evaluated on the skin at dosages of 0.65 and 1.7 mg of product/cm2, the latter dosage being the industrial standard for deet based repellents. The repellents were applied to the arm or lower leg of a human subject and tested against Anopheles stephensi in a cage or flying freely in a mosquito-proof room. In the cage tests, a product with 20% p-menthane-3, 8-diol (PMD) active ingredient provided complete repellency for 7-8 hours, while with 10% PMD had complete repellency for only 30 minutes. The natural oils of clove (Syzygium aromaticum) (10% active ingredient) plus makaen (Zanthoxylum limonella) (10% active ingredient) gave protection for 4-5 hours. In the case of free flying mosquitoes, products with 20% and 30% PMD gave complete protection for 11-12 hours at a dosage of 1.7 mg/cm2 or 6 hours at half the dosage, while the product with 10% PMD afforded protection for less than 2 hours. At the higher dosage rate 40% citronella and hydroxyethyl isobutyl piperidine carboxylate, a new synthetic compound, provided complete repellency for 7 hours. Fifty percent deet (N,N-diethyl-3-methylbenzamide) was effective for 30 hours if left undisturbed on the skin. </td></tr></tbody></table> <table class="css_list" id="pmid_16610643" name="pmid_16610643" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 Nov ;36:1412-22 16610643 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Screening for larvicidal activity in some Thai plants against four mosquito vector species. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Narumon Komalamisra , Yuwadee Trongtokit , Yupha Rongsriyam , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">Ninety-six ethanolic extracts from various parts of 84 Thai plant species were tested for their larvicidal activity against Aedes aegypti mosquitoes. Extracts from Rhinacanthus nasutus, Derris elliptica, Trigonostemon reidioides, Homalomena aromatica, Stemona tuberosa and Acorus calamus possessed high larvicidal activity, with LC50 values between 16.0 and 48.2 mg/l. Petroleum ether (PE) and methanol (MeOH) extracts were tested for their larvicidal activity against 4 mosquito vector species. The PE extract of R. nasutus exhibited larvicidal effects against Ae. aegypti, Culex quinquefasciatus, Anopheles dirus and Mansonia uniformis with LC50 values between 3.9 and 11.5 mg/l, while the MeOH extract gave LC50 values of between 8.1 and 14.7 mg/l. D. elliptica PE extract showed LC50 values of between 11.2 and 18.84 mg/l and the MeOH extract exhibited LC50 values between 13.2 and 45.2 mg/l. </td></tr></tbody></table> <table class="css_list" id="pmid_16438204" name="pmid_16438204" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 ;36 Suppl 4:167-75 16438204 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Three indigenous Thai medicinal plants for control of Aedes aegypti and Culex quinquefasciatus. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] P Lapcharoen , C Apiwathnasorn , N Komalamisra , P Dekumyoy , K Palakul , Y Rongsriyam </td></tr> <tr> <td colspan="2">The potential larvicidal activity and insect growth regulator (IGR) properties of three selected indigenous medicinal Thai plants were tested against two species of mosquito with special reference to the late 3rd and early 4th instar larvae (L3 and L4, respectively). In case of larvicidal activity, Thevetia peruviana was the most potent, followed by Pueraria mirifica, and Butea superba was the least effective. In all cases, the late 3rd instar was more susceptible than the early 4th instar larvae, and the 48-hours exposure yielded more potent larvicidal activity than 24-hours exposure. However, at sublethal dosages, both P. mirifica and B. superba showed some dispersed effects interfering with ecdysis. A variety of toxic effects were observed and recorded in eight categories according to the stage of metamorphosis when death occurred. P. mirifica rendered the main deleterious effects in the pupa-adult period in both instar of Ae. aegypti and Cx. quinquefasciatus, whereas B. superba showed highest effect in black-pupa period of the late 3rd instar larval stage. The results were reversed for the early 4th instar larvae of both species of mosquito as the main effect appeared in the pupa-adult category. The overall results indicated that T. peruviana did not show any IGR properties; whereas, P. mirifica and B. superba seemed to exhibit the juvenile hormone type activity which resulted in abnormal death at various stages of development. B. superba was more promising than P. mirifica, and Ae. aegypti was about 2 times more susceptible than Cx. quinquefasciatus. In addition, L3 was always more susceptible than L4 with both mosquito species. </td></tr></tbody></table> <table class="css_list" id="pmid_16041723" name="pmid_16041723" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Phytother Res. 2005 Apr ;19:303-9 16041723 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Comparative repellency of 38 essential oils against mosquito bites. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Yupha Rongsriyam , Narumon Komalamisra , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">The mosquito repellent activity of 38 essential oils from plants at three concentrations was screened against the mosquito Aedes aegypti under laboratory conditions using human subjects. On a volunteer's forearm, 0.1 mL of oil was applied per 30 cm2 of exposed skin. When the tested oils were applied at a 10% or 50% concentration, none of them prevented mosquito bites for as long as 2 h, but the undiluted oils of Cymbopogon nardus (citronella), Pogostemon cablin (patchuli), Syzygium aromaticum (clove) and Zanthoxylum limonella (Thai name: makaen) were the most effective and provided 2 h of complete repellency.From these initial results, three concentrations (10%, 50% and undiluted) of citronella, patchouli, clove and makaen were selected for repellency tests against Culex quinquefasciatus and Anopheles dirus. As expected, the undiluted oil showed the highest protection in each case. Clove oil gave the longest duration of 100% repellency (2-4 h) against all three species of mosquito. </td></tr> <tr class="css_mesh"> <td id="pmid_16041723" colspan="2" name="pmid_16041723">Mesh-terms: Administration, Cutaneous; Aedes; Animals; Anopheles; Comparative Study; Culex; Culicidae; Flowers; Humans; Insect Bites and Stings, prevention & control; Insect Repellents, administration & dosage; Insect Repellents, therapeutic use; Phytotherapy; Plant Leaves; Plant Oils, administration & dosage; Plant Oils, therapeutic use; Plants, Medicinal; Research Support, Non-U.S. Gov't; Seeds; Treatment Outcome; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15691131" name="pmid_15691131" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2004 Jun ;35:325-33 15691131 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Laboratory and field trial of developing medicinal local Thai plant products against four species of mosquito vectors. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Yupha Rongsriyam , Narumon Komalamisra , Panvipa Krisadaphong , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">Oils of Syzygium aromaticum (clove) and Zanthoxylum limonella (makaen), widely used essential oils for dental caries or flavoring of food in Thailand, were prepared as 10 experimental repellent products in gel or cream form against Aedes aegypti, Culex quinquefasciatus, and Anopheles dirus under laboratory conditions, using the human-arm-in-cage method. Two products that gave the longest-lasting complete protection were selected to examine their repellency against a variety of mosquito species under field conditions. In laboratory tests, 0.1 g of each product was applied to 3x10 cm of exposed area on a volunteer's forearm, while in field trials, 1.0 g was applied to each volunteer's leg (from knee to ankle). In the laboratory, the gel dosage form contained 20% clove oil (Gel B) or 10% clove plus 10% makaen oil mixture (Gel E) were promising plant-based repellents against three mosquito species and gave significantly longer complete protection times of 4-5 hours than all other developing products. Therefore, their efficacy in the field was evaluated. Under field conditions, Gel E showed complete protection for 4 hours and gave 95.7% repellency after 5 hours application, whereas Gel B and 20% deet (di-methyl benzamide) provided only 86.8 and 82.7% repellency after treatment, respectively against Ae. aegypti, daytime-biting mosquitos. For nighttime-biting, the 3 repellents under development yielded equally excellent (average 97.1%) repellency for 5 hours against the predominant Cx. quinquefasciatus and Mansonia uniformis, but they gave 89.0% repellency against Cx. tritaeniorhynchus and Cx. gelidus. This finding demonstrated the effectiveness of Gel B and Gel E products for possible use by low-income rural communities against various mosquito species. </td></tr> <tr class="css_mesh"> <td id="pmid_15691131" colspan="2" name="pmid_15691131">Mesh-terms: Animals; Clove Oil, administration & dosage; Clove Oil, chemistry; Comparative Study; Culicidae, drug effects; Drug Evaluation; Eugenia, chemistry; Humans; Insect Repellents, administration & dosage; Malaria, parasitology; Malaria, prevention & control; Mosquito Control, methods; Oils, Volatile, administration & dosage; Oils, Volatile, chemistry; Plant Oils, administration & dosage; Plant Oils, chemistry; Research Support, Non-U.S. Gov't; Thailand; Zanthoxylum, chemistry; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15115129" name="pmid_15115129" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2003 Sep ;34:564-8 15115129 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Seroepidemiological survey among schoolchildren during the 2000-2001 dengue outbreak of Ratchaburi Province, Thailand. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Walairut Tuntaprasart , Philippe Barbazan , Narong Nitatpattana , Yupha Rongsriyam , Sutee Yoksan , Jean Paul Gonzalez </td></tr> <tr> <td colspan="2">From August 2000 to 2001, a dengue outbreak occurred in Mueang district, Ratchaburi Province, Thailand. About 800 cases of dengue infection were reported, and among them, 49.5% were clinically diagnosed as dengue hemorrhagic fever according to the WHO criteria. During the outbreak, the incidence rate of dengue infection in Hin Gong subdistrict was 2.9 per 1,000 population. A seroepidemiological survey was conducted among primary schoolchildren from July 2000 to June 2001, to monitor dengue transmission. In a baseline survey, 283 children were surveyed for dengue antibody and 71% were IgG seropositive. In June 2001, the rate of dengue infection showed an increase of 8.8% with 8.0% among immune children and 10.3% among naive schoolchildren. Among 283 schoolchildren, 90 were followed up 3 times, in September and December 2000, and June 2001. An increase in the rate of seroconversion was observed in the period September to December 2000, while the peak dengue outbreaks in the dry season occurred in February 2001. Serosurveys among schoolchildren appear to be early warning system, and can be advantageous in early dengue control actions, in order to break the chain of transmission before an impending epidemic. </td></tr> <tr class="css_mesh"> <td id="pmid_15115129" colspan="2" name="pmid_15115129">Mesh-terms: Antibodies, Viral, blood; Child; Child, Preschool; Dengue, epidemiology; Dengue, immunology; Disease Outbreaks; Human; Immunoglobulin G, blood; Immunoglobulin M, blood; Longitudinal Studies; Seroepidemiologic Studies; Support, Non-U.S. Gov't; Thailand, epidemiology; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15115088" name="pmid_15115088" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2003 Dec ;34:786-92 15115088 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Temephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Pungasem Paeporn , Narumon Komalamisra , Vanida Deesin , Yupha Rongsriyam , Yuki E****a , Supatra Thongrungkiat </td></tr> <tr> <td colspan="2">Aedes aegypti, at the larval stage, has been subjected to the temephos selection in laboratory. The level of temephos resistance was detected in a microplate by biochemical assay using WHO bioassay technique. The major enzyme-based resistance mechanisms involved in temephos resistance include elevated nonspecific esterase, oxidase and insensitive acetylcholinesterase. After 19 generations of temephos selection, the selected group showed resistance ratios of 4.64 and 16.92, when compared with a non-selected group and the WHO susceptible strain, respectively. The two seperated forms, type form and the pale form of Ae. aegypti showed low levels of resistance to temephos after 19 generations of selection, with resistance ratios of 4.82 and 4.07 for the type form and the pale form, respectively; when compared with the non-selected strain, 17.58 and 14.84, when compared with the WHO susceptible strain. This showed that the type form could develop higher level resistance than the pale form. The esterase inhibitor (S,S,S-tributyl phosphorotrithioate, DEF) or synergist implicated detoxifying esterase in all the temephos selected groups and the presence of elevated esterase were confirmed by biochemical assay. There were significant differences in elevated esterase activity between the temephos selected groups and the non-selected group. However no significant difference between the type form and the pale form was found. Besides the elevated esterase, there was no change in monooxygenase activity and no evidence of insensitive acetylcholinesterease for all temephos selected groups. These results suggest that temephos resistance could be developed in Ae. aegypti under selection pressure and that the main mechanism is based only on esterase detoxification. </td></tr> <tr class="css_mesh"> <td id="pmid_15115088" colspan="2" name="pmid_15115088">Mesh-terms: Aedes, drug effects; Aedes, genetics; Animals; Dengue Hemorrhagic Fever, prevention & control; Insect Vectors, drug effects; Insect Vectors, genetics; Insecticide Resistance, genetics; Insecticide Resistance, physiology; Insecticides, Organothiophosphate, pharmacology; Larva, drug effects; Selection (Genetics); Temefos, pharmacology; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_12971471" name="pmid_12971471" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2002 ;33 Suppl 3:36-40 12971471 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Survey of indoor cockroaches in some dwellings in Bangkok. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] P Sriwichai , D Nacapunchai , S Pasuralertsakul , Y Rongsriyam , U Thavara </td></tr> <tr> <td colspan="2">This study aimed to investigate the species distribution of indoor cockroaches in dwellings in Bangkok. Commercial sticky traps were used to catch cockroaches in 128 private residences (40 single houses, 49 townhouses, 39 apartments), 34 offices, and 30 small groceries. The cockroaches were identified as Periplaneta americana, Supella longipalpa, Blattella germanica, Neostylopyga rhombifolia, P. brunnea, P. australasiae, Pycnoscelus surinamensis, and B. lituricallis; two were unidentified species. The most common species were P. americana, S. longipalpa and B. germanica--most of which were nymphal stages. P. americana and S. longipalpa infested all kinds of buildings, especially private residences (50.0% and 17.2%, respectively) and offices (61.8% and 58.8%, respectively); they were also found mostly (15.1%) in mixed infestation and with no predominant species. B. germanica (26.7%) were significantly predominant in groceries. Air conditioners have no influence on cockroach distribution. </td></tr> <tr class="css_mesh"> <td id="pmid_12971471" colspan="2" name="pmid_12971471">Mesh-terms: Animals; Cockroaches, classification; Cockroaches, growth & development; Housing; Population Dynamics; Species Specificity; Support, Non-U.S. Gov't; Thailand; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_12971470" name="pmid_12971470" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2002 ;33 Suppl 3:29-35 12971470 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Intraspecific hybridization of two karyotypic forms of Anopheles vagus (Diptera: Culicidae) and the related egg surface topography. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Wej Choochote , Atchariya Jitpakdi , Kom Sukontason , Udom Chaithong , Sirijit Wongkamchai , Benjawan Pitasawat , Narissara Jariyapan , Teerayut Suntaravitun , Eumporn Rattanachanpichai , Somjai Leemingsawat , Yupha Rongsriyam </td></tr> <tr> <td colspan="2">Hybridization tests of the two karyotypic forms (Form A and B) of laboratory-raised, isolines of Anopheles vagus, were conducted by induced copulation. The results of reciprocal- and back-crosses indicated that they were genetically compatible, providing viable progeny. Comparative egg morphometry and morphology, aided by scanning electron microscopy (SEM), revealed that the eggs of the two karyotypic forms were morphometrically and morphologically similar. </td></tr> <tr class="css_mesh"> <td id="pmid_12971470" colspan="2" name="pmid_12971470">Mesh-terms: Animals; Anopheles, classification; Anopheles, genetics; Anopheles, physiology; Hybridization, Genetic; Karyotyping; Microscopy, Electron, Scanning; Ovum, ultrastructure; Support, Non-U.S. Gov't; </td> </tr><tr></tr></tbody></table>
http://rongsriyam.lib.bioinfo.pl/auth:Rongsriyam,Y
_______________________________
Latest Paper:
<table class="css_list" id="pmid_17124984" name="pmid_17124984" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2006 Mar ;37(2):265-71 17124984 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Formulation of tablets from the crude extract of Rhinacanthus nasutus (Thai local plant) against Aedes aegypti and Culex quinquefasciatus larvae: a preliminary study. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yupha Rongsriyam , Yuwadee Trongtokit , Narumon Komalamisra , Nuttanan Sinchaipanich , Chamnarn Apiwathnasorn , Aumpol Mitrejet </td></tr> <tr> <td colspan="2">Dried root powder of Rhinacanthus nasutus, Thong Phan Chang (Thai name) were extracted with methanol (MeOH) in a Soxhlet apparatus and made into 2 formulations of tablet containing the extract at 5% and 10% concentration. Due to the viscous and poor flow properties of the crude MeOH extract obtained, a wet granulation method was conducted in developing the tablets. Lactose was used as a filler. Polyvinyl pyrrolidone (PVP) K30 (15% w/w solution in alcohol) was used as the binding agent, while stearic acid (2% w/w) was used as a lubricant. Both formulas of prepared tablets had a smooth shiny surface with a round shape. Other physical properties of the tablets, such as weight variation, friability and disintegration time, met the requirements of the USP XX standard. The mosquito larvicidal activity of prepared tablets containing 5% and 10% R. nasutus extract against Aedes aegypti were not significantly different from each other (p > 0.05), with 48-hour LC50 values of 13.6 and 14.2 mg/I for the 5% and 10% tablets, respectively, while their activities against Culex quinquefasciatus were similar (p > 0.05) with LC50 values of 18.7 and 17.3, respectively. The larvicidal activity levels against Ae. aegypti and Cx. quinquefasciatus were also not significantly different from each other (p > 0.05). No larval mortality was observed in the two control groups: lactose solution and dechlorinated water. Toxicity to female and male fish (Poecilia reticulata) was tested with the prepared tablets. The toxicity of tablets containing 5% and 10% extracts were not significantly different from each other for the P. reticulata females with 48-hour LC50 values of 105.2 and 110.8 mg/I, respectively, and for P. reticulata males with LC50 values of 99.1 and 103.4 mg/I, respectively. Female and male P. reticulata were sensitive to the same dose of the extract. No fish died in the two control groups, with lactose solution and dechlorinated water. Acute-toxicity bioassay with fish showed that with an exposure of 48 hours the LC50 values of the tablets containing 5% and 10% were 5- to 10-fold higher than the LC50 of R. nasutus against mosquito larvae. These prepared tablets could possibly used to control mosquito vectors and be introduced into the mosquito control program. </td></tr></tbody></table> <table class="css_list" id="pmid_16610644" name="pmid_16610644" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 Nov ;36:1423-31 16610644 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Efficacy of repellent products against caged and free flying Anopheles stephensi mosquitoes. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Christopher F Curtis , Yupha Rongsriyam </td></tr> <tr> <td colspan="2">The efficacy of 9 repellents (8 commercial repellents and one product under development) was evaluated on the skin at dosages of 0.65 and 1.7 mg of product/cm2, the latter dosage being the industrial standard for deet based repellents. The repellents were applied to the arm or lower leg of a human subject and tested against Anopheles stephensi in a cage or flying freely in a mosquito-proof room. In the cage tests, a product with 20% p-menthane-3, 8-diol (PMD) active ingredient provided complete repellency for 7-8 hours, while with 10% PMD had complete repellency for only 30 minutes. The natural oils of clove (Syzygium aromaticum) (10% active ingredient) plus makaen (Zanthoxylum limonella) (10% active ingredient) gave protection for 4-5 hours. In the case of free flying mosquitoes, products with 20% and 30% PMD gave complete protection for 11-12 hours at a dosage of 1.7 mg/cm2 or 6 hours at half the dosage, while the product with 10% PMD afforded protection for less than 2 hours. At the higher dosage rate 40% citronella and hydroxyethyl isobutyl piperidine carboxylate, a new synthetic compound, provided complete repellency for 7 hours. Fifty percent deet (N,N-diethyl-3-methylbenzamide) was effective for 30 hours if left undisturbed on the skin. </td></tr></tbody></table> <table class="css_list" id="pmid_16610643" name="pmid_16610643" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 Nov ;36:1412-22 16610643 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Screening for larvicidal activity in some Thai plants against four mosquito vector species. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Narumon Komalamisra , Yuwadee Trongtokit , Yupha Rongsriyam , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">Ninety-six ethanolic extracts from various parts of 84 Thai plant species were tested for their larvicidal activity against Aedes aegypti mosquitoes. Extracts from Rhinacanthus nasutus, Derris elliptica, Trigonostemon reidioides, Homalomena aromatica, Stemona tuberosa and Acorus calamus possessed high larvicidal activity, with LC50 values between 16.0 and 48.2 mg/l. Petroleum ether (PE) and methanol (MeOH) extracts were tested for their larvicidal activity against 4 mosquito vector species. The PE extract of R. nasutus exhibited larvicidal effects against Ae. aegypti, Culex quinquefasciatus, Anopheles dirus and Mansonia uniformis with LC50 values between 3.9 and 11.5 mg/l, while the MeOH extract gave LC50 values of between 8.1 and 14.7 mg/l. D. elliptica PE extract showed LC50 values of between 11.2 and 18.84 mg/l and the MeOH extract exhibited LC50 values between 13.2 and 45.2 mg/l. </td></tr></tbody></table> <table class="css_list" id="pmid_16438204" name="pmid_16438204" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2005 ;36 Suppl 4:167-75 16438204 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Three indigenous Thai medicinal plants for control of Aedes aegypti and Culex quinquefasciatus. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] P Lapcharoen , C Apiwathnasorn , N Komalamisra , P Dekumyoy , K Palakul , Y Rongsriyam </td></tr> <tr> <td colspan="2">The potential larvicidal activity and insect growth regulator (IGR) properties of three selected indigenous medicinal Thai plants were tested against two species of mosquito with special reference to the late 3rd and early 4th instar larvae (L3 and L4, respectively). In case of larvicidal activity, Thevetia peruviana was the most potent, followed by Pueraria mirifica, and Butea superba was the least effective. In all cases, the late 3rd instar was more susceptible than the early 4th instar larvae, and the 48-hours exposure yielded more potent larvicidal activity than 24-hours exposure. However, at sublethal dosages, both P. mirifica and B. superba showed some dispersed effects interfering with ecdysis. A variety of toxic effects were observed and recorded in eight categories according to the stage of metamorphosis when death occurred. P. mirifica rendered the main deleterious effects in the pupa-adult period in both instar of Ae. aegypti and Cx. quinquefasciatus, whereas B. superba showed highest effect in black-pupa period of the late 3rd instar larval stage. The results were reversed for the early 4th instar larvae of both species of mosquito as the main effect appeared in the pupa-adult category. The overall results indicated that T. peruviana did not show any IGR properties; whereas, P. mirifica and B. superba seemed to exhibit the juvenile hormone type activity which resulted in abnormal death at various stages of development. B. superba was more promising than P. mirifica, and Ae. aegypti was about 2 times more susceptible than Cx. quinquefasciatus. In addition, L3 was always more susceptible than L4 with both mosquito species. </td></tr></tbody></table> <table class="css_list" id="pmid_16041723" name="pmid_16041723" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Phytother Res. 2005 Apr ;19:303-9 16041723 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Comparative repellency of 38 essential oils against mosquito bites. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Yupha Rongsriyam , Narumon Komalamisra , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">The mosquito repellent activity of 38 essential oils from plants at three concentrations was screened against the mosquito Aedes aegypti under laboratory conditions using human subjects. On a volunteer's forearm, 0.1 mL of oil was applied per 30 cm2 of exposed skin. When the tested oils were applied at a 10% or 50% concentration, none of them prevented mosquito bites for as long as 2 h, but the undiluted oils of Cymbopogon nardus (citronella), Pogostemon cablin (patchuli), Syzygium aromaticum (clove) and Zanthoxylum limonella (Thai name: makaen) were the most effective and provided 2 h of complete repellency.From these initial results, three concentrations (10%, 50% and undiluted) of citronella, patchouli, clove and makaen were selected for repellency tests against Culex quinquefasciatus and Anopheles dirus. As expected, the undiluted oil showed the highest protection in each case. Clove oil gave the longest duration of 100% repellency (2-4 h) against all three species of mosquito. </td></tr> <tr class="css_mesh"> <td id="pmid_16041723" colspan="2" name="pmid_16041723">Mesh-terms: Administration, Cutaneous; Aedes; Animals; Anopheles; Comparative Study; Culex; Culicidae; Flowers; Humans; Insect Bites and Stings, prevention & control; Insect Repellents, administration & dosage; Insect Repellents, therapeutic use; Phytotherapy; Plant Leaves; Plant Oils, administration & dosage; Plant Oils, therapeutic use; Plants, Medicinal; Research Support, Non-U.S. Gov't; Seeds; Treatment Outcome; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15691131" name="pmid_15691131" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2004 Jun ;35:325-33 15691131 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Laboratory and field trial of developing medicinal local Thai plant products against four species of mosquito vectors. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Yuwadee Trongtokit , Yupha Rongsriyam , Narumon Komalamisra , Panvipa Krisadaphong , Chamnarn Apiwathnasorn </td></tr> <tr> <td colspan="2">Oils of Syzygium aromaticum (clove) and Zanthoxylum limonella (makaen), widely used essential oils for dental caries or flavoring of food in Thailand, were prepared as 10 experimental repellent products in gel or cream form against Aedes aegypti, Culex quinquefasciatus, and Anopheles dirus under laboratory conditions, using the human-arm-in-cage method. Two products that gave the longest-lasting complete protection were selected to examine their repellency against a variety of mosquito species under field conditions. In laboratory tests, 0.1 g of each product was applied to 3x10 cm of exposed area on a volunteer's forearm, while in field trials, 1.0 g was applied to each volunteer's leg (from knee to ankle). In the laboratory, the gel dosage form contained 20% clove oil (Gel B) or 10% clove plus 10% makaen oil mixture (Gel E) were promising plant-based repellents against three mosquito species and gave significantly longer complete protection times of 4-5 hours than all other developing products. Therefore, their efficacy in the field was evaluated. Under field conditions, Gel E showed complete protection for 4 hours and gave 95.7% repellency after 5 hours application, whereas Gel B and 20% deet (di-methyl benzamide) provided only 86.8 and 82.7% repellency after treatment, respectively against Ae. aegypti, daytime-biting mosquitos. For nighttime-biting, the 3 repellents under development yielded equally excellent (average 97.1%) repellency for 5 hours against the predominant Cx. quinquefasciatus and Mansonia uniformis, but they gave 89.0% repellency against Cx. tritaeniorhynchus and Cx. gelidus. This finding demonstrated the effectiveness of Gel B and Gel E products for possible use by low-income rural communities against various mosquito species. </td></tr> <tr class="css_mesh"> <td id="pmid_15691131" colspan="2" name="pmid_15691131">Mesh-terms: Animals; Clove Oil, administration & dosage; Clove Oil, chemistry; Comparative Study; Culicidae, drug effects; Drug Evaluation; Eugenia, chemistry; Humans; Insect Repellents, administration & dosage; Malaria, parasitology; Malaria, prevention & control; Mosquito Control, methods; Oils, Volatile, administration & dosage; Oils, Volatile, chemistry; Plant Oils, administration & dosage; Plant Oils, chemistry; Research Support, Non-U.S. Gov't; Thailand; Zanthoxylum, chemistry; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15115129" name="pmid_15115129" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2003 Sep ;34:564-8 15115129 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Seroepidemiological survey among schoolchildren during the 2000-2001 dengue outbreak of Ratchaburi Province, Thailand. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Walairut Tuntaprasart , Philippe Barbazan , Narong Nitatpattana , Yupha Rongsriyam , Sutee Yoksan , Jean Paul Gonzalez </td></tr> <tr> <td colspan="2">From August 2000 to 2001, a dengue outbreak occurred in Mueang district, Ratchaburi Province, Thailand. About 800 cases of dengue infection were reported, and among them, 49.5% were clinically diagnosed as dengue hemorrhagic fever according to the WHO criteria. During the outbreak, the incidence rate of dengue infection in Hin Gong subdistrict was 2.9 per 1,000 population. A seroepidemiological survey was conducted among primary schoolchildren from July 2000 to June 2001, to monitor dengue transmission. In a baseline survey, 283 children were surveyed for dengue antibody and 71% were IgG seropositive. In June 2001, the rate of dengue infection showed an increase of 8.8% with 8.0% among immune children and 10.3% among naive schoolchildren. Among 283 schoolchildren, 90 were followed up 3 times, in September and December 2000, and June 2001. An increase in the rate of seroconversion was observed in the period September to December 2000, while the peak dengue outbreaks in the dry season occurred in February 2001. Serosurveys among schoolchildren appear to be early warning system, and can be advantageous in early dengue control actions, in order to break the chain of transmission before an impending epidemic. </td></tr> <tr class="css_mesh"> <td id="pmid_15115129" colspan="2" name="pmid_15115129">Mesh-terms: Antibodies, Viral, blood; Child; Child, Preschool; Dengue, epidemiology; Dengue, immunology; Disease Outbreaks; Human; Immunoglobulin G, blood; Immunoglobulin M, blood; Longitudinal Studies; Seroepidemiologic Studies; Support, Non-U.S. Gov't; Thailand, epidemiology; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_15115088" name="pmid_15115088" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2003 Dec ;34:786-92 15115088 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Temephos resistance in two forms of Aedes aegypti and its significance for the resistance mechanism. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Pungasem Paeporn , Narumon Komalamisra , Vanida Deesin , Yupha Rongsriyam , Yuki E****a , Supatra Thongrungkiat </td></tr> <tr> <td colspan="2">Aedes aegypti, at the larval stage, has been subjected to the temephos selection in laboratory. The level of temephos resistance was detected in a microplate by biochemical assay using WHO bioassay technique. The major enzyme-based resistance mechanisms involved in temephos resistance include elevated nonspecific esterase, oxidase and insensitive acetylcholinesterase. After 19 generations of temephos selection, the selected group showed resistance ratios of 4.64 and 16.92, when compared with a non-selected group and the WHO susceptible strain, respectively. The two seperated forms, type form and the pale form of Ae. aegypti showed low levels of resistance to temephos after 19 generations of selection, with resistance ratios of 4.82 and 4.07 for the type form and the pale form, respectively; when compared with the non-selected strain, 17.58 and 14.84, when compared with the WHO susceptible strain. This showed that the type form could develop higher level resistance than the pale form. The esterase inhibitor (S,S,S-tributyl phosphorotrithioate, DEF) or synergist implicated detoxifying esterase in all the temephos selected groups and the presence of elevated esterase were confirmed by biochemical assay. There were significant differences in elevated esterase activity between the temephos selected groups and the non-selected group. However no significant difference between the type form and the pale form was found. Besides the elevated esterase, there was no change in monooxygenase activity and no evidence of insensitive acetylcholinesterease for all temephos selected groups. These results suggest that temephos resistance could be developed in Ae. aegypti under selection pressure and that the main mechanism is based only on esterase detoxification. </td></tr> <tr class="css_mesh"> <td id="pmid_15115088" colspan="2" name="pmid_15115088">Mesh-terms: Aedes, drug effects; Aedes, genetics; Animals; Dengue Hemorrhagic Fever, prevention & control; Insect Vectors, drug effects; Insect Vectors, genetics; Insecticide Resistance, genetics; Insecticide Resistance, physiology; Insecticides, Organothiophosphate, pharmacology; Larva, drug effects; Selection (Genetics); Temefos, pharmacology; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_12971471" name="pmid_12971471" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2002 ;33 Suppl 3:36-40 12971471 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Survey of indoor cockroaches in some dwellings in Bangkok. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] P Sriwichai , D Nacapunchai , S Pasuralertsakul , Y Rongsriyam , U Thavara </td></tr> <tr> <td colspan="2">This study aimed to investigate the species distribution of indoor cockroaches in dwellings in Bangkok. Commercial sticky traps were used to catch cockroaches in 128 private residences (40 single houses, 49 townhouses, 39 apartments), 34 offices, and 30 small groceries. The cockroaches were identified as Periplaneta americana, Supella longipalpa, Blattella germanica, Neostylopyga rhombifolia, P. brunnea, P. australasiae, Pycnoscelus surinamensis, and B. lituricallis; two were unidentified species. The most common species were P. americana, S. longipalpa and B. germanica--most of which were nymphal stages. P. americana and S. longipalpa infested all kinds of buildings, especially private residences (50.0% and 17.2%, respectively) and offices (61.8% and 58.8%, respectively); they were also found mostly (15.1%) in mixed infestation and with no predominant species. B. germanica (26.7%) were significantly predominant in groceries. Air conditioners have no influence on cockroach distribution. </td></tr> <tr class="css_mesh"> <td id="pmid_12971471" colspan="2" name="pmid_12971471">Mesh-terms: Animals; Cockroaches, classification; Cockroaches, growth & development; Housing; Population Dynamics; Species Specificity; Support, Non-U.S. Gov't; Thailand; </td> </tr><tr></tr></tbody></table> <table class="css_list" id="pmid_12971470" name="pmid_12971470" cellpadding="0" cellspacing="0"> <tbody> <tr class="css_cit"> <td class="css_cit">Southeast Asian J Trop Med Public Health. 2002 ;33 Suppl 3:29-35 12971470 </td> <td class="css_right">
</td></tr> <tr> <td colspan="2">Intraspecific hybridization of two karyotypic forms of Anopheles vagus (Diptera: Culicidae) and the related egg surface topography. </td></tr> <tr class="css_cit"> <td colspan="2">[My paper] Wej Choochote , Atchariya Jitpakdi , Kom Sukontason , Udom Chaithong , Sirijit Wongkamchai , Benjawan Pitasawat , Narissara Jariyapan , Teerayut Suntaravitun , Eumporn Rattanachanpichai , Somjai Leemingsawat , Yupha Rongsriyam </td></tr> <tr> <td colspan="2">Hybridization tests of the two karyotypic forms (Form A and B) of laboratory-raised, isolines of Anopheles vagus, were conducted by induced copulation. The results of reciprocal- and back-crosses indicated that they were genetically compatible, providing viable progeny. Comparative egg morphometry and morphology, aided by scanning electron microscopy (SEM), revealed that the eggs of the two karyotypic forms were morphometrically and morphologically similar. </td></tr> <tr class="css_mesh"> <td id="pmid_12971470" colspan="2" name="pmid_12971470">Mesh-terms: Animals; Anopheles, classification; Anopheles, genetics; Anopheles, physiology; Hybridization, Genetic; Karyotyping; Microscopy, Electron, Scanning; Ovum, ultrastructure; Support, Non-U.S. Gov't; </td> </tr><tr></tr></tbody></table>