Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing

[Mary Wilson]

NOTE: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.

Accepted Date: 24 March 2021
Published: March 31, 2021
DOI:https://doi.org/10.1016/j.medj.2021.03.015

• Chongwei Bi, Gerardo Ramos-Mandujano, Yeteng Tian, Sharif Hala, Jinna Xu, Sara Mfarrej, Concepcion Rodriguez Esteban, Estrella Nu?ez Delicado, Fadwa S. Alofi, Asim Khogeer, Anwar M. Hashem, Naif A.M. Almontashiri, Arnab Pain, Juan Carlos Izpisua Belmonte, Mo Li

Highlights

• Multiplex isothermal amplification of viral genomes followed by Nanopore sequencing
• Simultaneous detection of SARS-CoV-2 and co-infecting viruses in up to 96 patients
• Simultaneous detection of SARS-CoV-2 and pepper mild mottle virus in wastewater
• Real-time and field-deployable mutational surveillance of SARS-CoV-2 variants

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https://www.cell.com/med/fulltext/S2666-6340(21)00117-3