tetano
Editor, Senior Moderator
PLoS One. 2013 May 21;8(5):e64017. doi: 10.1371/journal.pone.0064017. Print 2013.
Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid.
Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T, Kato N.
Source
The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
Abstract
To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
PMID:
23704970
[PubMed - in process]
PMCID:
PMC3660326
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23704970
Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid.
Kaihatsu K, Sawada S, Nakamura S, Nakaya T, Yasunaga T, Kato N.
Source
The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
Abstract
To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
PMID:
23704970
[PubMed - in process]
PMCID:
PMC3660326
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/23704970