tetano
Editor, Senior Moderator
Virology. 2017 Feb 17;505:23-32. doi: 10.1016/j.virol.2017.02.008. [Epub ahead of print]
[h=1]Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein.[/h] Chaimayo C[SUP]1[/SUP], Hayashi T[SUP]2[/SUP], Underwood A[SUP]2[/SUP], Hodges E[SUP]2[/SUP], Takimoto T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Assembly; CRM1; CRNP; Influenza; M1; Nuclear export; Trafficking; VRNP
PMID: 28219018 DOI: 10.1016/j.virol.2017.02.008
[PubMed - as supplied by publisher]
[h=1]Selective incorporation of vRNP into influenza A virions determined by its specific interaction with M1 protein.[/h] Chaimayo C[SUP]1[/SUP], Hayashi T[SUP]2[/SUP], Underwood A[SUP]2[/SUP], Hodges E[SUP]2[/SUP], Takimoto T[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza A viruses contain eight single-stranded, negative-sense RNA segments as viral genomes in the form of viral ribonucleoproteins (vRNPs). During genome replication in the nucleus, positive-sense complementary RNPs (cRNPs) are produced as replicative intermediates, which are not incorporated into progeny virions. To analyze the mechanism of selective vRNP incorporation into progeny virions, we quantified vRNPs and cRNPs in the nuclear and cytosolic fractions of infected cells, using a strand-specific qRT-PCR. Unexpectedly, we found that cRNPs were also exported to the cytoplasm. This export was chromosome region maintenance 1 (CRM1)-independent unlike that of vRNPs. Although both vRNPs and cRNPs were present in the cytosol, viral matrix (M1) protein, a key regulator for viral assembly, preferentially bound vRNPs over cRNPs. These results indicate that influenza A viruses selectively uptake cytosolic vRNPs through a specific interaction with M1 during viral assembly.
Copyright ? 2017 Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Assembly; CRM1; CRNP; Influenza; M1; Nuclear export; Trafficking; VRNP
PMID: 28219018 DOI: 10.1016/j.virol.2017.02.008
[PubMed - as supplied by publisher]