tetano
Editor, Senior Moderator
Biochem J. 2016 Sep 30. pii: BCJ20160651. [Epub ahead of print]
[h=1]Secondary structure model of the naked segment 7 influenza A virus genomic RNA.[/h] Ruszkowska A[SUP]1[/SUP], Lenartowicz E[SUP]2[/SUP], Moss WN[SUP]3[/SUP], Kierzek R[SUP]2[/SUP], Kierzek E[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus genome is comprised of eight negative-sense viral (v)RNA segments. The seventh segment of the genome encodes two essential viral proteins and is specifically packaged alongside the other seven vRNAs. To gain insight into the possible roles of RNA structure both within and without virions, a secondary structure model of a naked (protein-free) segment 7 vRNA has been determined using chemical mapping and thermodynamic energy minimization. The proposed structure model was validated using microarray mapping, RNase H cleavage and comparative sequence analysis. Additionally, the detailed structures of three segment 7 vRNA fragment constructs-comprising independently-folded sub-domains-were determined. Much of the proposed 7 vRNA structure is preserved between IAV strains, suggesting their importance in the influenza replication cycle. Possible structure rearrangements, which allow or preclude long range RNA interactions are also proposed.
?2016 The Author(s).
[h=4]KEYWORDS:[/h] Influenza A Virus; RNA segmented genome; RNA structure; chemical probing; microarray mapping
PMID: 27694388 DOI: 10.1042/BCJ20160651
[PubMed - as supplied by publisher]
[h=1]Secondary structure model of the naked segment 7 influenza A virus genomic RNA.[/h] Ruszkowska A[SUP]1[/SUP], Lenartowicz E[SUP]2[/SUP], Moss WN[SUP]3[/SUP], Kierzek R[SUP]2[/SUP], Kierzek E[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza A virus genome is comprised of eight negative-sense viral (v)RNA segments. The seventh segment of the genome encodes two essential viral proteins and is specifically packaged alongside the other seven vRNAs. To gain insight into the possible roles of RNA structure both within and without virions, a secondary structure model of a naked (protein-free) segment 7 vRNA has been determined using chemical mapping and thermodynamic energy minimization. The proposed structure model was validated using microarray mapping, RNase H cleavage and comparative sequence analysis. Additionally, the detailed structures of three segment 7 vRNA fragment constructs-comprising independently-folded sub-domains-were determined. Much of the proposed 7 vRNA structure is preserved between IAV strains, suggesting their importance in the influenza replication cycle. Possible structure rearrangements, which allow or preclude long range RNA interactions are also proposed.
?2016 The Author(s).
[h=4]KEYWORDS:[/h] Influenza A Virus; RNA segmented genome; RNA structure; chemical probing; microarray mapping
PMID: 27694388 DOI: 10.1042/BCJ20160651
[PubMed - as supplied by publisher]