tetano
Editor, Senior Moderator
Science. 2020 May 1. pii: eabc1669. doi: 10.1126/science.abc1669. [Epub ahead of print]
SARS-CoV-2 productively infects human gut enterocytes.
Lamers MM[SUP]#[/SUP][SUP]1[/SUP], Beumer J[SUP]#[/SUP][SUP]2[/SUP], van der Vaart J[SUP]#[/SUP][SUP]2[/SUP], Knoops K[SUP]3[/SUP], Puschhof J[SUP]2[/SUP], Breugem TI[SUP]1[/SUP], Ravelli RBG[SUP]3[/SUP], Paul van Schayck J[SUP]3[/SUP], Mykytyn AZ[SUP]1[/SUP], Duimel HQ[SUP]3[/SUP], van Donselaar E[SUP]3[/SUP], Riesebosch S[SUP]1[/SUP], Kuijpers HJH[SUP]3[/SUP], Schippers D[SUP]1[/SUP], van de Wetering WJ[SUP]3[/SUP], de Graaf M[SUP]1[/SUP], Koopmans M[SUP]1[/SUP], Cuppen E[SUP]4,[/SUP][SUP]5[/SUP], Peters PJ[SUP]3[/SUP], Haagmans BL[SUP]#[/SUP][SUP]1[/SUP], Clevers H[SUP]#[/SUP][SUP]6[/SUP].
Author information
Abstract
The virus severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission via the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2 as demonstrated by confocal- and electron-microscopy. Consequently, significant titers of infectious viral particles were detected. mRNA expression analysis revealed strong induction of a generic viral response program. Hence, intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
Copyright ? 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID:32358202DOI:10.1126/science.abc1669
SARS-CoV-2 productively infects human gut enterocytes.
Lamers MM[SUP]#[/SUP][SUP]1[/SUP], Beumer J[SUP]#[/SUP][SUP]2[/SUP], van der Vaart J[SUP]#[/SUP][SUP]2[/SUP], Knoops K[SUP]3[/SUP], Puschhof J[SUP]2[/SUP], Breugem TI[SUP]1[/SUP], Ravelli RBG[SUP]3[/SUP], Paul van Schayck J[SUP]3[/SUP], Mykytyn AZ[SUP]1[/SUP], Duimel HQ[SUP]3[/SUP], van Donselaar E[SUP]3[/SUP], Riesebosch S[SUP]1[/SUP], Kuijpers HJH[SUP]3[/SUP], Schippers D[SUP]1[/SUP], van de Wetering WJ[SUP]3[/SUP], de Graaf M[SUP]1[/SUP], Koopmans M[SUP]1[/SUP], Cuppen E[SUP]4,[/SUP][SUP]5[/SUP], Peters PJ[SUP]3[/SUP], Haagmans BL[SUP]#[/SUP][SUP]1[/SUP], Clevers H[SUP]#[/SUP][SUP]6[/SUP].
Author information
Abstract
The virus severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission via the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin converting enzyme 2 (ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2 as demonstrated by confocal- and electron-microscopy. Consequently, significant titers of infectious viral particles were detected. mRNA expression analysis revealed strong induction of a generic viral response program. Hence, intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
Copyright ? 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
PMID:32358202DOI:10.1126/science.abc1669