tetano
Editor, Senior Moderator
Sci Rep
. 2022 Apr 14;12(1):5804.
doi: 10.1038/s41598-022-09402-7.
Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO [SUB]2[/SUB]-based photocatalyst
Ryuichi Nakano[SUP] 1 [/SUP], Akira Yamaguchi[SUP] 2 [/SUP], Kayano Sunada[SUP] 3 [/SUP], Takeshi Nagai[SUP] 3 [/SUP], Akiyo Nakano[SUP] 4 [/SUP], Yuki Suzuki[SUP] 4 [/SUP], Hisakazu Yano[SUP] 4 [/SUP], Hitoshi Ishiguro[SUP] 5 [/SUP], Masahiro Miyauchi[SUP] 6 [/SUP]
Affiliations
Abstract
Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB]) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night.
. 2022 Apr 14;12(1):5804.
doi: 10.1038/s41598-022-09402-7.
Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO [SUB]2[/SUB]-based photocatalyst
Ryuichi Nakano[SUP] 1 [/SUP], Akira Yamaguchi[SUP] 2 [/SUP], Kayano Sunada[SUP] 3 [/SUP], Takeshi Nagai[SUP] 3 [/SUP], Akiyo Nakano[SUP] 4 [/SUP], Yuki Suzuki[SUP] 4 [/SUP], Hisakazu Yano[SUP] 4 [/SUP], Hitoshi Ishiguro[SUP] 5 [/SUP], Masahiro Miyauchi[SUP] 6 [/SUP]
Affiliations
- PMID: 35422456
- DOI: 10.1038/s41598-022-09402-7
Abstract
Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB]) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The Cu[SUB]x[/SUB]O/TiO[SUB]2[/SUB] photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night.