tetano
Editor, Senior Moderator
Sci Rep
. 2023 Jul 18;13(1):11610.
doi: 10.1038/s41598-023-38548-1. Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells
Shohei Minami[SUP] 1 [/SUP], Naomi Matsumoto[SUP] 1 [/SUP], Hiroko Omori[SUP] 2 [/SUP], Yutaka Nakamura[SUP] 3 [/SUP], Shigeyuki Tamiya[SUP] 3 [/SUP], Ryotaro Nouda[SUP] 1 [/SUP], Jeffery A Nurdin[SUP] 1 [/SUP], Moeko Yamasaki[SUP] 1 [/SUP], Tomohiro Kotaki[SUP] 1 [/SUP], Yuta Kanai[SUP] 1 [/SUP], Toru Okamoto[SUP] 4 5 [/SUP], Taro Tachibana[SUP] 6 7 [/SUP], Hiroshi Ushijima[SUP] 8 [/SUP], Takeshi Kobayashi[SUP] 9 10 [/SUP], Shintaro Sato[SUP] 11 12 [/SUP]
Affiliations
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.
. 2023 Jul 18;13(1):11610.
doi: 10.1038/s41598-023-38548-1. Effective SARS-CoV-2 replication of monolayers of intestinal epithelial cells differentiated from human induced pluripotent stem cells
Shohei Minami[SUP] 1 [/SUP], Naomi Matsumoto[SUP] 1 [/SUP], Hiroko Omori[SUP] 2 [/SUP], Yutaka Nakamura[SUP] 3 [/SUP], Shigeyuki Tamiya[SUP] 3 [/SUP], Ryotaro Nouda[SUP] 1 [/SUP], Jeffery A Nurdin[SUP] 1 [/SUP], Moeko Yamasaki[SUP] 1 [/SUP], Tomohiro Kotaki[SUP] 1 [/SUP], Yuta Kanai[SUP] 1 [/SUP], Toru Okamoto[SUP] 4 5 [/SUP], Taro Tachibana[SUP] 6 7 [/SUP], Hiroshi Ushijima[SUP] 8 [/SUP], Takeshi Kobayashi[SUP] 9 10 [/SUP], Shintaro Sato[SUP] 11 12 [/SUP]
Affiliations
- PMID: 37463955
- DOI: 10.1038/s41598-023-38548-1
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.