tetano
Editor, Senior Moderator
Sci Rep
. 2020 Nov 30;10(1):20836.
doi: 10.1038/s41598-020-77945-8.
Depletion of circulating IgM memory B cells predicts unfavourable outcome in COVID-19
Marco Vincenzo Lenti[SUP] 1 [/SUP], Nicola Aronico[SUP] 1 [/SUP], Ivan Pellegrino[SUP] 1 [/SUP], Emanuela Boveri[SUP] 2 [/SUP], Paolo Giuffrida[SUP] 1 [/SUP], Federica Borrelli de Andreis[SUP] 1 [/SUP], Patrizia Morbini[SUP] 2 [/SUP], Laura Vanelli[SUP] 3 [/SUP], Alessandra Pasini[SUP] 1 [/SUP], Cristina Ubezio[SUP] 1 [/SUP], Federica Melazzini[SUP] 1 [/SUP], Alessandro Rascaroli[SUP] 1 [/SUP], Valentina Antoci[SUP] 1 [/SUP], Stefania Merli[SUP] 1 [/SUP], Francesco Di Terlizzi[SUP] 1 [/SUP], Umberto Sabatini[SUP] 1 [/SUP], Ginevra Cambi?[SUP] 1 [/SUP], Annamaria Tenore[SUP] 3 [/SUP], Cristina Picone[SUP] 3 [/SUP], Alessandro Vanoli[SUP] 2 [/SUP], Luca Arcaini[SUP] 3 [/SUP], Fausto Baldanti[SUP] 4 [/SUP], Marco Paulli[SUP] 2 [/SUP], Gino Roberto Corazza[SUP] 1 [/SUP], Antonio Di Sabatino[SUP] 5 6 [/SUP]
Affiliations
Abstract
Impaired immune responses have been hypothesised to be a possible trigger of unfavourable outcomes in coronavirus disease 2019 (COVID-19). We aimed to characterise IgM memory B cells in patients with COVID-19 admitted to an internal medicine ward in Northern Italy. Overall, 66 COVID-19 patients (mean age 74 ? 16.6 years; 29 females) were enrolled. Three patients (4.5%; 1 female) had been splenectomised and were excluded from further analyses. Fifty-five patients (87.3%) had IgM memory B cell depletion, and 18 (28.6%) died during hospitalisation (cumulative incidence rate 9.26/100 person-week; 5.8-14.7 95% CI). All patients who died had IgM memory B cell depletion. A superimposed infection was found in 6 patients (9.5%), all of them having IgM memory B cell depletion (cumulative incidence rate 3.08/100 person-week; 1.3-6.8 95% CI). At bivariable analyses, older age, sex, number of comorbidities, and peripheral blood lymphocyte count < 1500/?l were not correlated with IgM memory B cell depletion. A discrete-to-marked reduction of the B-cell compartment was also noticed in autoptic spleen specimens of two COVID-19 patients. We conclude that IgM memory B cells are commonly depleted in COVID-19 patients and this correlates with increased mortality and superimposed infections.
. 2020 Nov 30;10(1):20836.
doi: 10.1038/s41598-020-77945-8.
Depletion of circulating IgM memory B cells predicts unfavourable outcome in COVID-19
Marco Vincenzo Lenti[SUP] 1 [/SUP], Nicola Aronico[SUP] 1 [/SUP], Ivan Pellegrino[SUP] 1 [/SUP], Emanuela Boveri[SUP] 2 [/SUP], Paolo Giuffrida[SUP] 1 [/SUP], Federica Borrelli de Andreis[SUP] 1 [/SUP], Patrizia Morbini[SUP] 2 [/SUP], Laura Vanelli[SUP] 3 [/SUP], Alessandra Pasini[SUP] 1 [/SUP], Cristina Ubezio[SUP] 1 [/SUP], Federica Melazzini[SUP] 1 [/SUP], Alessandro Rascaroli[SUP] 1 [/SUP], Valentina Antoci[SUP] 1 [/SUP], Stefania Merli[SUP] 1 [/SUP], Francesco Di Terlizzi[SUP] 1 [/SUP], Umberto Sabatini[SUP] 1 [/SUP], Ginevra Cambi?[SUP] 1 [/SUP], Annamaria Tenore[SUP] 3 [/SUP], Cristina Picone[SUP] 3 [/SUP], Alessandro Vanoli[SUP] 2 [/SUP], Luca Arcaini[SUP] 3 [/SUP], Fausto Baldanti[SUP] 4 [/SUP], Marco Paulli[SUP] 2 [/SUP], Gino Roberto Corazza[SUP] 1 [/SUP], Antonio Di Sabatino[SUP] 5 6 [/SUP]
Affiliations
- PMID: 33257775
- DOI: 10.1038/s41598-020-77945-8
Abstract
Impaired immune responses have been hypothesised to be a possible trigger of unfavourable outcomes in coronavirus disease 2019 (COVID-19). We aimed to characterise IgM memory B cells in patients with COVID-19 admitted to an internal medicine ward in Northern Italy. Overall, 66 COVID-19 patients (mean age 74 ? 16.6 years; 29 females) were enrolled. Three patients (4.5%; 1 female) had been splenectomised and were excluded from further analyses. Fifty-five patients (87.3%) had IgM memory B cell depletion, and 18 (28.6%) died during hospitalisation (cumulative incidence rate 9.26/100 person-week; 5.8-14.7 95% CI). All patients who died had IgM memory B cell depletion. A superimposed infection was found in 6 patients (9.5%), all of them having IgM memory B cell depletion (cumulative incidence rate 3.08/100 person-week; 1.3-6.8 95% CI). At bivariable analyses, older age, sex, number of comorbidities, and peripheral blood lymphocyte count < 1500/?l were not correlated with IgM memory B cell depletion. A discrete-to-marked reduction of the B-cell compartment was also noticed in autoptic spleen specimens of two COVID-19 patients. We conclude that IgM memory B cells are commonly depleted in COVID-19 patients and this correlates with increased mortality and superimposed infections.